Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000445.1:
Cluster 1NRP164650

BGC0000445, thiocoraline biosynthetic gene cluster from Micromonospora sp. ML1. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: thiocoraline
SMILES string: Copy to clipboard
Molecular formula: C48H56N10O12S6
Exact molecular mass: 1158.41 Da ([M+H]+)
Molecular activity: Cytotoxic
Molecular target: DNA polymerase alpha

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Type I & II
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
tioR (CAJ34374.1)
Module 1
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
AA is epimerized
C domain subtype: Unknown
Module 2
A specificity: Glycine
Evidence for specificity: Sequence-based prediction
C domain subtype: Unknown
tioS (CAJ34375.1)
Module 3
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
C domain subtype: Unknown
Scaffold-modifying domain: Methylation
Module 4
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
C domain subtype: Unknown
Scaffold-modifying domain: Methylation
tioL (CAJ34368.1)
Module 0
A specificity: None
Evidence for specificity: Unknown
C domain subtype: Unknown

Gene cluster description

thiocoraline (BGC0000445). Gene Cluster 1. Biosynthetic class = NRP. GenBank AM113472. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jose A. Salas (University of Oviedo)

Literature references

1. Lombo F et al. (2006) Deciphering the biosynthesis pathway of the antitumor thiocoraline from a marine actinomycete and its expression in two streptomyces species. Chembiochem 7(2):366-76. doi: 10.1002/cbic.200500325.