Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000447.1:
Cluster 1NRP169124

BGC0000447, tolaasin biosynthetic gene cluster from Pseudomonas costantinii. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: tolaasin I
PubChem ID: 44559556
ChemSpider ID: 23327101
SMILES string: Copy to clipboard
Molecular formula: C94H163N21O25
Average molecular mass: 1987.46 Da
Molecular activity: Antifungal
Molecular target: cell membrane

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
beta-hydroxy-octanoic acid
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
taaA (CCJ67636.1)
Module 1
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: Starter
Scaffold-modifying domain: dehydration
Module 2
A specificity: Proline
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 3
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
taaB (CCJ67637.1)
Module 4
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 5
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 6
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
taaC (CCJ67638.1)
Module 7
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 8
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 9
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
taaD (CCJ67639.1)
Module 10
A specificity: Glutamine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 11
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 12
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: Other
taaE (CCJ67640.1)
Module 13
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Scaffold-modifying domain: dehydration
Module 14
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 15
A specificity: Leucine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
Module 16
A specificity: Homoserine
Evidence for specificity: Structure-based inference
Amino acid biosynthesis subcluster:
C domain subtype: LCL
Module 17
A specificity: 2,4-Diaminobutyric acid
Evidence for specificity: Sequence-based prediction
Amino acid biosynthesis subcluster:
C domain subtype: LCL
Module 18
A specificity: Lysine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL

Gene cluster description

tolaasin (BGC0000447). Gene Cluster 1. Biosynthetic class = NRP. GenBank HE967327. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Keishi Ishida (Leipniz Institute for Natural Product Research and Infection Biology)

Literature references

1. Scherlach K et al. (2013) Biosynthesis and mass spectrometric imaging of tolaasin, the virulence factor of brown blotch mushroom disease. Chembiochem 14(18):2439-43. doi: 10.1002/cbic.201300553. Epub 2013
2. Jo G et al. (2011) In silico study of the ion channel formed by tolaasin I produced by Pseudomonas tolaasii. J Microbiol Biotechnol 21(10):1097-100.
3. Cho KH et al. (2007) Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264. J Biochem Mol Biol 40(1):113-8.
4. Bassarello C et al. (2004) Tolaasins A--E, five new lipodepsipeptides produced by Pseudomonas tolaasii. J Nat Prod 67(5):811-6. doi: 10.1021/np0303557.