Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000459.1:
Cluster 1NRP / Polyketide1104671

BGC0000459, griseoviridin / viridogrisein biosynthetic gene cluster from Streptomyces griseoviridis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: griseoviridin
SMILES string: Copy to clipboard
Molecular formula: C22H27N3O7S
Exact molecular mass: 477.157 Da (M+)
Molecular activity: Antibacterial
Molecular target: A site of 50S bacterial ribosomal subunit

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Cyclic depsipeptide (cyclic)
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
sgvD1 (AGN74880.1)
Module 0
A specificity: 3-hydroxypicolinic acid
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
sgvD2 (AGN74876.1)
Module 1
A specificity: Threonine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 2
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
sgvD3 (AGN74886.1)
Module A3
A specificity: 4-Hydroxyproline
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: DCL
sgvD4 (AGN74885.1)
Module 4
A specificity: Sarcosine
Evidence for specificity: Structure-based inference
C domain subtype: DCL
Scaffold-modifying domain: Methylation
Module 5
A specificity: Beta-methylleucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
Module 6
A specificity: Alanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module A7
A specificity: Phenylglycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
sgvE1 (AGN74892.1)
Module B3
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: Other
sgvE4 (AGN74895.1)
Module B7
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
Scaffold-modifying domain: Other
sgvE6 (AGN74898.1)
Module B9
A specificity: Cysteine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
sgvE5 (AGN74896.1)
Module B10
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Other
Module is skipped
Evidence for skipping: Unknown
Module 9
A specificity: Unknown
Evidence for specificity: Unknown
AA is epimerized
C domain subtype: Other
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Trans-AT type I
Starter unit:
Acetyl-CoA
SGVQ
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
sgvE1 (AGN74892.1)
Module 0
Scaffold-modifying domain: None
Module B1
KR stereochemistry: A-group
Scaffold-modifying domain: None
Module B2
KR stereochemistry: A-group
Scaffold-modifying domain: None

sgvE2 (AGN74893.1)
Module B4
KR stereochemistry: A-group
Scaffold-modifying domain: None

sgvE3 (AGN74894.1)
Module B5
KR stereochemistry: B-group
Scaffold-modifying domain: None
Module B6
Scaffold-modifying domain: None

sgvE4 (AGN74895.1)
Module B8
Scaffold-modifying domain: None

Gene cluster description

griseoviridin / viridogrisein (BGC0000459). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank JX508597, positions 13530-118200. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Comments:VG is synthesised by the NRPS within this cluster whereas GV is hybrid PK-NRP and as such utilises the PKS machinery also.
Contact for this cluster:Jianhua Ju (Guangdong Key Laboratory of Marine Materia Medica Deputy director, Key Laboratory of Marine Bio-resources Sustainable Utilization,CAS South China Sea Institute of Oceanology, Chinese Academy of Sciences (CAS) 164 XinGang Road West Guangzhou 510301, China)

Literature references

1. Xie Y et al. (2012) Identification of the biosynthetic gene cluster and regulatory cascade for the synergistic antibacterial antibiotics griseoviridin and viridogrisein in Streptomyces griseoviridis. Chembiochem 13(18):2745-57. doi: 10.1002/cbic.201200584. Epub 2012
2. Xie Y et al. (2014) Involvement of SgvP in carbon-sulfur bond formation during Griseoviridin biosynthesis. Chembiochem 15(8):1183-9. doi: 10.1002/cbic.201400062. Epub 2014 Apr
3. Xie Y, Ju J. (2013) [Substrate specificity of 3-hydroxypicolinic acid unit in the viridogrisein biosynthetic pathway]. Wei Sheng Wu Xue Bao 53(11):1179-88.