BGC0000893

BGC0000893: chloramphenicol biosynthetic gene cluster from Streptomyces venezuelae ATCC 10712

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Location: 1,039,846 - 1,061,851 nt. (total: 22,006 nt).
This entry is originally from NCBI GenBank FR845719.1.

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Gene details

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General

Compounds

Genes

NRP

History

NRPS/PKS domains

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000893
Short description	chloramphenicol biosynthetic gene cluster from Streptomyces venezuelae ATCC 10712
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks	"Chloramphenicol gene cluster comprises 17 genes (sven_0913 to sven_0929) including a monomodular NRPS. "
Biosynthetic class(es)	NRP
Loci	NCBI GenBank: FR845719.1
Compounds	chloramphenicol
Species	Streptomyces venezuelae ATCC 10712 [taxonomy]
References	The gene cluster for chloramphenicol biosynthesis in Streptomyces venezuelae ISP5230 includes novel shikimate pathway homologues and a monomodular non-ribosomal peptide synthetase gene. He J et al., Microbiology (Reading) (2001) PMID:11577160 New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712. Fernández-Martínez LT et al., Antimicrob Agents Chemother (2014) PMID:25267678 Revisiting the structures of several antibiotics bound to the bacterial ribosome. Bulkley D et al., Proc Natl Acad Sci U S A (2010) PMID:20876130 Para-position derivatives of fungal anthelmintic cyclodepsipeptides engineered with Streptomyces venezuelae antibiotic biosynthetic genes. Yanai K et al., Nat Biotechnol (2004) PMID:15184904 A family of diiron monooxygenases catalyzing amino acid beta-hydroxylation in antibiotic biosynthesis. Makris TM et al., Proc Natl Acad Sci U S A (2010) PMID:20713732 CmlI is an . Lu H et al., Tetrahedron (2012) PMID:24347692 Expression, purification and preliminary diffraction studies of CmlS. Latimer R et al., Acta Crystallogr Sect F Struct Biol Cryst Commun (2009) PMID:19255478 Biosynthesis of the dichloroacetyl component of chloramphenicol in Streptomyces venezuelae ISP5230: genes required for halogenation. Piraee M et al., Microbiology (Reading) (2004) PMID:14702400

Chemical products information

chloramphenicol

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C₁₁H₁₂Cl₂N₂O₅

Chemical database entries
NPAtlas
PubCHEM
ChemSpider

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
SVEN_0913 CCA54200.1	1039846 - 1040880 (+)	CmlR	Regulation	Knock-out	copy AA seq copy Nt seq
SVEN_0914 CCA54201.1	1040885 - 1041604 (-)	CmlL	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
SVEN_0915 CCA54202.1	1041601 - 1043019 (-)	CmlN	Transport	Knock-out	copy AA seq copy Nt seq
SVEN_0916 CCA54203.1	1043332 - 1044543 (+)	CmlF	Transport	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0917 CCA54204.1	1044647 - 1045909 (-)	CmlE	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0918 CCA54205.1	1045906 - 1046217 (-)	CmlD	Scaffold biosynthesis	Other in vivo study	copy AA seq copy Nt seq
SVEN_0919 CCA54206.1	1046214 - 1047200 (-)	CmlC	Scaffold biosynthesis	Other in vivo study	copy AA seq copy Nt seq
SVEN_0920 CCA54207.1	1047197 - 1049257 (-)	CmlB	Scaffold biosynthesis	Other in vivo study	copy AA seq copy Nt seq
SVEN_0921 CCA54208.1	1049401 - 1050999 (-)	CmlA	Scaffold biosynthesis	Activity assay	copy AA seq copy Nt seq
SVEN_0922 CCA54209.1	1051023 - 1053914 (-)	CmlP	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0923 CCA54210.1	1053898 - 1055076 (-)	CmlH	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0924 CCA54211.1	1055073 - 1056092 (-)	CmlI	Scaffold biosynthesis	Activity assay	copy AA seq copy Nt seq
SVEN_0925 CCA54212.1	1056366 - 1056692 (+)	CmlM	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0926 CCA54213.1	1056710 - 1057408 (+)	CmlJ	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0927 CCA54214.1	1057408 - 1058853 (+)	CmlK	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
SVEN_0928 CCA54215.1	1058957 - 1060672 (+)	CmlS	Tailoring (Halogenation)	Activity assay	copy AA seq copy Nt seq
SVEN_0929 CCA54216.1	1060871 - 1061851 (+)	CmlT	Unknown	Knock-out	copy AA seq copy Nt seq

NRP-specific information

Subclass	N/A
Cyclic?	no
Release type	Reductive release
Thioesterase genes	SVEN_0922 (Unknown)

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: RJXZFYIPTBJ27AV3IUUT4BRL, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4 Updated compound(s) information (MIBiG Annotathon) Updated compound(s) information (NPAtlas curation)
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Removed duplicate thioesterase Updated bioactivity data
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

Detailed domain annotation

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