Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000953.1:
Cluster 1Saccharide137337

BGC0000953, amicetin biosynthetic gene cluster from Streptomyces vinaceusdrappus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: amicetin
PubChem ID: 221187
SMILES string: Copy to clipboard
Molecular formula: C29H42N6O9
Average molecular mass: 618.688 Da
Molecular activity: Antibacterial
Molecular target: RNA polymerase

Class-specific details

Biosynthetic class(es):
Saccharide
Saccharide subclass:
aminoglycoside
Glycosyltransferases:
Unknown gene ID
Specificity: Other
Evidence for specificity: Activity assay

Gene cluster description

amicetin (BGC0000953). Gene Cluster 1. Biosynthetic class = Saccharide. GenBank HM748814, positions 7917-34889. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Changsheng Zhang (South China Sea Institute of Oceanology, Chinese Academy of Sciences)

Literature references

1. Zhang G et al. (2012) Characterization of the amicetin biosynthesis gene cluster from Streptomyces vinaceusdrappus NRRL 2363 implicates two alternative strategies for amide bond formation. Appl Environ Microbiol 78(7):2393-401. doi: 10.1128/AEM.07185-11. Epub
2. Hu T et al. (2012) [Cloning, expression and characterization of the nucleotidylyltransferase gene-amiE in amicetin biosynthesis]. Wei Sheng Wu Xue Bao 52(2):214-20.
3. Chen R et al. (2013) Characterizing amosamine biosynthesis in amicetin reveals AmiG as a reversible retaining glycosyltransferase. J Am Chem Soc 135(33):12152-5. doi: 10.1021/ja401016e. Epub 2013 Aug