Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000954.1:
Cluster 1NRP / Polyketide183663

BGC0000954, ajudazol biosynthetic gene cluster from Chondromyces crocatus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Ajudazol
SMILES string: Copy to clipboard
Molecular formula: C34H42N2O7
Exact molecular mass: 591.3065 Da ([M+H]+)
Molecular activity: Antifungal

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Other

Nonribosomal peptide synthetases:
ajuD (CAQ18831.1)
Module 8
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
ajuL (CAQ18839.1)
Module 2
A specificity: N-Methyl-Glycine
Evidence for specificity: Structure-based inference
Amino acid biosynthesis subcluster:
C domain subtype: DCL
Scaffold-modifying domain: Methylation
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Acetyl-CoA
Thioesterase type:
Type II
Release / cyclization type:
Other

Modular polyketide synthases:
ajuA (CAQ18828.1)
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

ajuB (CAQ18829.1)
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group

ajuC (CAQ18830.1)
Module 7
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
KR stereochemistry: B-group

ajuE (CAQ18832.1)
Module 9
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Sequence-based prediction
KR stereochemistry: B-group

ajuF (CAQ18833.1)
Module 10
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

ajuG (CAQ18834.1)
Module 11
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Module 12
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group

ajuH (CAQ18835.1)
Module 13
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference

ajuK (CAQ18838.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: Methylation

ajuL (CAQ18839.1)
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

Gene cluster description

ajudazol (BGC0000954). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AM946600, positions 1-83663. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:No
Contact for this cluster:Daniel Krug (HIPS)

Literature references

1. Buntin K et al. (2010) An unusual thioesterase promotes isochromanone ring formation in ajudazol biosynthesis. Chembiochem 11(8):1137-46. doi: 10.1002/cbic.200900712.