BGC0000954 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000954 (Chondromyces crocatus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	83,663	stigmatellin	NRP + Polyketide:Modular type I	45%

BGC0000954 - Region 1 - NRPS,T1PKS

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Location: 1 - 83,663 nt. (total: 83,663 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 83,664
Label		BGC0000954

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000954.1	1.40	NRP, Polyketide	ajudazol A	Chondromyces crocatus
BGC0001068.1	0.76	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.73	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000041.1	0.72	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000056.1	0.72	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0001023.1	0.67	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0002057.1	0.66	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0001135.1	0.65	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001844.1	0.65	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0000979.1	0.65	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000954.1	0.90	NRP, Polyketide	ajudazol A	Chondromyces crocatus
BGC0001068.1	0.63	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.63	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000041.1	0.61	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001023.1	0.60	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0000465.1	0.58	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000389.1	0.58	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0000979.1	0.57	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.1	0.57	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0002071.1	0.57	NRP	Virginiafactin	Pseudomonas sp. QS1027

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 83663:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (redmal) + (ohmal - ccmal) + (pk) + (cys) + (Me-redmal) + (ohmal) + (mal - Me-ccmal) + (mal) + (NMe-gly - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ajuA

PKS_AT (565..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.9
inactive: 147.4
Methylmalonyl-CoA: 80.5
Methoxymalonyl-CoA: 75.5
Propionyl-CoA: 56.5
Ethylmalonyl-CoA: 36.7
2-Methylbutyryl-CoA: 32.3
Isobutyryl-CoA: 29.7
trans-1,2-CPDA: 29.4
CHC-CoA: 29.1
Benzoyl-CoA: 27.1
Acetyl-CoA: 26.9
fatty_acid: 15.9
3-Methylbutyryl-CoA: 9.6

ajuB

PKS_AT (574..867): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.3
inactive: 121.3
Methoxymalonyl-CoA: 86.3
Methylmalonyl-CoA: 81.1
Propionyl-CoA: 68.6
Ethylmalonyl-CoA: 45.2
Isobutyryl-CoA: 43.7
2-Methylbutyryl-CoA: 36.6
Acetyl-CoA: 36.0
Benzoyl-CoA: 30.4
trans-1,2-CPDA: 29.1
CHC-CoA: 28.8
fatty_acid: 28.4
3-Methylbutyryl-CoA: 20.4

PKS_AT (2068..2361): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.3
inactive: 121.3
Methoxymalonyl-CoA: 86.3
Methylmalonyl-CoA: 81.1
Propionyl-CoA: 68.6
Ethylmalonyl-CoA: 45.2
Isobutyryl-CoA: 43.7
2-Methylbutyryl-CoA: 36.6
Acetyl-CoA: 36.0
Benzoyl-CoA: 30.4
trans-1,2-CPDA: 29.1
CHC-CoA: 28.8
fatty_acid: 28.4
3-Methylbutyryl-CoA: 20.4

ajuC

PKS_AT (570..869): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 194.6
Methylmalonyl-CoA: 192.4
Methoxymalonyl-CoA: 159.3
2-Methylbutyryl-CoA: 110.0
trans-1,2-CPDA: 106.7
Malonyl-CoA: 97.5
Isobutyryl-CoA: 91.7
Propionyl-CoA: 91.5
Benzoyl-CoA: 81.4
CHC-CoA: 62.8
Acetyl-CoA: 36.6
3-Methylbutyryl-CoA: 33.5
fatty_acid: 33.0
inactive: 29.7

ajuD

Search NORINE for peptide: strict or relaxed

AMP-binding (542..941): cys

NRPSPredictor2: cys

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNlaLVWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 80% (moderate)

ajuE

PKS_AT (535..834): mmal

ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 181.7
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 144.8
Malonyl-CoA: 109.9
Benzoyl-CoA: 99.6
2-Methylbutyryl-CoA: 94.6
Isobutyryl-CoA: 87.9
trans-1,2-CPDA: 82.3
Propionyl-CoA: 78.5
Acetyl-CoA: 63.2
CHC-CoA: 60.3
fatty_acid: 39.9
3-Methylbutyryl-CoA: 37.3
inactive: 33.4

ajuF

PKS_AT (567..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.5
inactive: 128.0
Methoxymalonyl-CoA: 79.7
Methylmalonyl-CoA: 78.4
Propionyl-CoA: 53.7
Isobutyryl-CoA: 52.3
Benzoyl-CoA: 42.8
Ethylmalonyl-CoA: 41.5
2-Methylbutyryl-CoA: 36.8
trans-1,2-CPDA: 33.5
CHC-CoA: 30.4
fatty_acid: 29.9
Acetyl-CoA: 27.6
3-Methylbutyryl-CoA: 15.9

ajuG

PKS_AT (565..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.7
inactive: 147.9
Methoxymalonyl-CoA: 76.3
Methylmalonyl-CoA: 75.6
Propionyl-CoA: 67.9
2-Methylbutyryl-CoA: 43.4
Isobutyryl-CoA: 41.8
Ethylmalonyl-CoA: 40.2
Acetyl-CoA: 34.7
trans-1,2-CPDA: 30.3
Benzoyl-CoA: 30.2
CHC-CoA: 29.2
fatty_acid: 17.0
3-Methylbutyryl-CoA: 10.1

PKS_AT (1602..1899): mmal

ATSignature: Propionyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Propionyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.7
Ethylmalonyl-CoA: 144.7
Methoxymalonyl-CoA: 120.9
Isobutyryl-CoA: 96.2
Malonyl-CoA: 87.3
2-Methylbutyryl-CoA: 86.8
Propionyl-CoA: 82.8
Acetyl-CoA: 71.9
CHC-CoA: 70.3
trans-1,2-CPDA: 69.2
Benzoyl-CoA: 68.1
3-Methylbutyryl-CoA: 45.4
inactive: 40.8
fatty_acid: 29.1

ajuH

PKS_AT (568..865): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.4
inactive: 110.5
Methoxymalonyl-CoA: 85.2
Methylmalonyl-CoA: 84.9
Propionyl-CoA: 61.7
Ethylmalonyl-CoA: 59.7
2-Methylbutyryl-CoA: 55.6
CHC-CoA: 42.4
Isobutyryl-CoA: 34.3
trans-1,2-CPDA: 32.5
Acetyl-CoA: 31.2
Benzoyl-CoA: 27.6
fatty_acid: 20.1
3-Methylbutyryl-CoA: 11.7

ajuK

PKS_AT (616..912): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 118.4
Methoxymalonyl-CoA: 108.8
Malonyl-CoA: 99.2
Ethylmalonyl-CoA: 95.8
Isobutyryl-CoA: 86.3
Propionyl-CoA: 66.9
2-Methylbutyryl-CoA: 57.6
inactive: 56.0
Acetyl-CoA: 53.6
trans-1,2-CPDA: 53.5
Benzoyl-CoA: 43.6
CHC-CoA: 36.2
3-Methylbutyryl-CoA: 33.4
fatty_acid: 21.0

PKS_AT (1057..1355): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Methylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 126.1
Methylmalonyl-CoA: 124.4
Methoxymalonyl-CoA: 108.6
Ethylmalonyl-CoA: 89.3
Isobutyryl-CoA: 79.8
Propionyl-CoA: 78.0
2-Methylbutyryl-CoA: 74.9
CHC-CoA: 67.5
Benzoyl-CoA: 62.7
trans-1,2-CPDA: 55.8
inactive: 47.6
3-Methylbutyryl-CoA: 44.0
Acetyl-CoA: 43.2
fatty_acid: 37.0

ajuL

Search NORINE for peptide: strict or relaxed

AMP-binding (530..928): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

PKS_AT (2069..2374): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.6
inactive: 134.2
Methoxymalonyl-CoA: 76.3
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 69.3
Ethylmalonyl-CoA: 37.7
2-Methylbutyryl-CoA: 37.1
Isobutyryl-CoA: 28.7
CHC-CoA: 27.9
Benzoyl-CoA: 27.3
fatty_acid: 26.0
Acetyl-CoA: 24.0
trans-1,2-CPDA: 22.4
3-Methylbutyryl-CoA: 7.0