Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000956.1:
Cluster 1NRP / Polyketide128485

BGC0000956, andrimid biosynthetic gene cluster from Pantoea agglomerans. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: andrimid
PubChem ID: 636857
SMILES string: Copy to clipboard
Molecular formula: C27H33N3O5
Average molecular mass: 479.577 Da
Molecular activity: Antibacterial
Molecular target: beta-subunit of acetyl-CoA carboxylase

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Beta-lactam (linear)
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Polyketide subclass:
Ansamycin (linear)
Polyketide synthase subclass:
Type II
Starter unit:
Malonyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
AAO39098.1

Gene cluster description

andrimid (BGC0000956). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AY192157, positions 1-28485. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Antonio Ruzzini (Harvard Medical School)

Literature references

1. Freiberg C et al. (2004) Identification and characterization of the first class of potent bacterial acetyl-CoA carboxylase inhibitors with antibacterial activity. J Biol Chem 279(25):26066-73. doi: 10.1074/jbc.M402989200. Epub 2004
2. Jin M et al. (2006) A biosynthetic gene cluster for the acetyl-CoA carboxylase inhibitor andrimid. J Am Chem Soc 128(33):10660-1. doi: 10.1021/ja063194c.
3. Fortin PD et al. (2007) A transglutaminase homologue as a condensation catalyst in antibiotic assembly lines. Nature 448(7155):824-7. doi: 10.1038/nature06068. Epub 2007 Jul 25.
4. Magarvey NA et al. (2008) Gatekeeping versus promiscuity in the early stages of the andrimid biosynthetic assembly line. ACS Chem Biol 3(9):542-54. doi: 10.1021/cb800085g. Epub 2008 Jul 25.
5. Liu X et al. (2008) Andrimid producers encode an acetyl-CoA carboxyltransferase subunit resistant to the action of the antibiotic. Proc Natl Acad Sci U S A 105(36):13321-6. doi:
6. Ratnayake ND et al. (2011) Stereochemistry and mechanism of a microbial phenylalanine aminomutase. J Am Chem Soc 133(22):8531-3. doi: 10.1021/ja2030728. Epub 2011 May