Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000962.1:
Cluster 1NRP / Polyketide140156

BGC0000962, barbamide biosynthetic gene cluster from Lyngbya majuscula. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: barbamide
SMILES string: Copy to clipboard
Molecular formula: C20H23Cl3N2O2S
Exact molecular mass: 460.0546 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
barA (AAN32974.1)
Module X
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
barC (AAN32977.1)
Module 0
A specificity: Leucine
Evidence for specificity: Activity assay
C domain subtype: N/A
barD (AAN32978.1)
Module 1
A specificity: (4S)-5,5,5-trichloroleucine
Evidence for specificity: Activity assay
C domain subtype: N/A
barE (AAN32979.1)
Module 2
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
Scaffold-modifying domain: Methylation
barF (AAN32980.1)
Module 3
A specificity: Phenylalanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
Module 4
A specificity: Cysteine
Evidence for specificity: Activity assay
C domain subtype: LCL
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Malonyl-CoA
Thioesterase type:
None
Release / cyclization type:
Unknown

Modular polyketide synthases:
barD (AAN32978.1)
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Scaffold-modifying domain: None

Gene cluster description

barbamide (BGC0000962). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AF516145, positions 1-40156. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Fengan Yu (University of Michigan)

Literature references

1. Chang Z et al. (2002) The barbamide biosynthetic gene cluster: a novel marine cyanobacterial system of mixed polyketide synthase (PKS)-non-ribosomal peptide synthetase (NRPS) origin involving an unusual trichloroleucyl starter unit. Gene 296(1-2):235-47.