BGC0000985: didemnin B biosynthetic gene cluster from Tistrella mobilis KA081020-065
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 654,941 - 749,400 nt. (total: 94,460 nt).
This entry is originally from NCBI GenBank CP003239.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000985
Short description didemnin B biosynthetic gene cluster from Tistrella mobilis KA081020-065
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "Analysis of genes flanking didA-J, and within the Tistrella mobilis genome, did not reveal a discrete AT suggesting the Didemnin polyketide synthase may use the fatty acid synthase FabD"
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
Loci
CP003239.1
674941 - 729697
via
Compounds
  • didemnin B
  • nordidemnin B
  • dehydrodidemnin B (aplidine)
  • didemnin X
  • didemnin Y
Species Tistrella mobilis KA081020-065 [taxonomy]
References
Chemical products information
didemnin B Evidence:
Copy SMILES
C57H89N7O15
Chemical database entries
PubCHEM
nordidemnin B Evidence:
Copy SMILES
C56H87N7O15
Chemical database entries
PubCHEM
PubCHEM
dehydrodidemnin B (aplidine) Evidence:
Copy SMILES
C56H87N7O15
didemnin X Evidence:
Copy SMILES
C82H130N12O24
Chemical database entries
PubCHEM
didemnin Y Evidence:
Copy SMILES
C87H138N14O26
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • TMO_c0602
  • AFK57212.1
  • didA
 674941 - 681312 (+) DidA
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0603
  • AFK57213.1
  • didB
 681309 - 686699 (+) DidB
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0604
  • AFK57214.1
  • didC
 686696 - 690688 (+) DidC
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0605
  • AFK57215.1
  • didD
 690664 - 702225 (+) DidD
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0606
  • AFK57216.1
  • didE
 702250 - 707367 (+) DidE
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0607
  • AFK57217.1
  • didF
 707383 - 712224 (+) DidF
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0608
  • AFK57218.1
  • didG
 712221 - 716462 (+) DidG
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0609
  • AFK57219.1
  • didH
 716459 - 720319 (+) DidH
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0610
  • AFK57220.1
  • didI
 720291 - 722912 (+) DidI
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0611
  • AFK57221.1
  • didJ
 722909 - 729400 (+) DidJ
  • Scaffold biosynthesis
copy AA seq
copy Nt seq
  • TMO_c0612
  • AFK57222.1
  • mbtH
 729464 - 729697 (+) MbtH domain-containing protein
  • Activation / processing
copy AA seq
copy Nt seq
Biosynthesis information

Biosynthetic modules

Name
Unk01
Type
nrps-type1
Genes
didA
Substrates
glutamine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk02
Type
nrps-type1
Genes
didA
Substrates
glutamine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk03
Type
nrps-type1
Genes
didC
Substrates
proline (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk04
Type
nrps-type1
Genes
didD
Substrates
leucine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk05
Type
nrps-type1
Genes
didD
Substrates
threonine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk06
Type
nrps-type1
Genes
didD
Substrates
isoleucine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk07
Type
nrps-type1
Genes
didH
Substrates
leucine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk08
Type
nrps-type1
Genes
didI
Substrates
proline (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Name
Unk09
Type
nrps-type1
Genes
didJ
Substrates
tyrosine (evidence: Structure-based inference, Sequence-based prediction)
Integrated Monomers
Domains
adenylation
Annotation changelog

Entry version: 4

Date
Changes
Submitters
Reviewers
2022-10-07
Update chemical activity to schema version 2.11
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date
Changes
Submitters
Reviewers
2022-09-15
Cleaned up synonym list
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Update compound structure (SMILES) for nordidemnin B
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Linked new PubChem id for nordidemnin B (pubchem:164049)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Add new compound: dehydrodidemnin B (aplidine)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Updated bioactivity data
  • (ID: 5UL74VURKJ25VSPPPO3H2NYB)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Added NRP substrate specificities
  • (ID: 5UL74VURKJ25VSPPPO3H2NYB)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date
Changes
Submitters
Reviewers
2019-10-16
Migrated from v1.4
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2019-10-16
Updated compound(s) information (MIBiG Annotathon)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2019-10-16
Updated compound(s) information (NPAtlas curation)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date
Changes
Submitters
Reviewers
2015-06-12
Submitted
  • (ID: FC27FLXW4TUBR6P2D2MHHJOO)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
Similar known clusters from MIBiG 4.0
Shows clusters from the MIBiG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Detailed help and explanations are available here.

Click on reference genes to show details of similarities to genes within the current region.
Double click on a reference drawing to reverse the display of the genes.

Click on an accession to open that entry in the MIBiG database.


Location:

Identifier
Identity
Coverage
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A domain glossary is available here, and an explanation of the visualisation is available here.
Selected features only
Show module domains