BGC0000989 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000989 (Sorangium cellulosum So0157-2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	56,019	epothilone	NRP + Polyketide	70%

BGC0000989 - Region 1 - NRPS,T1PKS

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Location: 1 - 56,019 nt. (total: 56,019 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 56,020
Label		BGC0000989

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000989.1	1.12	NRP, Polyketide	epothilone	Sorangium cellulosum So0157-2
BGC0000991.1	0.96	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000990.1	0.95	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000988.1	0.86	NRP, Polyketide	epothilone B	Sorangium cellulosum
BGC0001164.1	0.56	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002057.1	0.56	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0000041.1	0.49	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001235.1	0.49	NRP, Polyketide	antalid	Polyangium spumosum
BGC0001012.1	0.46	NRP, Polyketide	meridamycin	Streptomyces violaceusniger
BGC0001162.1	0.46	Polyketide	1-heptadecene	Moorea producens JHB

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000989.1	0.92	NRP, Polyketide	epothilone	Sorangium cellulosum So0157-2
BGC0000991.1	0.86	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000990.1	0.86	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000988.1	0.83	NRP, Polyketide	epothilone B	Sorangium cellulosum
BGC0001164.1	0.68	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001068.1	0.63	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000067.1	0.63	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002057.1	0.62	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0000041.1	0.60	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001012.1	0.60	NRP, Polyketide	meridamycin	Streptomyces violaceusniger

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 56019:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (cys) + (Me-ccmal) + (ohmal - ohmal - redmal - Me-redmal) + (Me-ohmal - Me-Me-ccmal) + (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

epoA

PKS_AT (543..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.4
inactive: 132.5
Methoxymalonyl-CoA: 59.2
Propionyl-CoA: 47.2
Methylmalonyl-CoA: 44.3
Isobutyryl-CoA: 30.9
2-Methylbutyryl-CoA: 28.1
Acetyl-CoA: 25.3
Benzoyl-CoA: 24.7
Ethylmalonyl-CoA: 18.9
CHC-CoA: 13.6
fatty_acid: 11.2
3-Methylbutyryl-CoA: 9.1
trans-1,2-CPDA: 8.4

epoP

Search NORINE for peptide: strict or relaxed

AMP-binding (528..930): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

epoB

PKS_AT (539..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.1
Ethylmalonyl-CoA: 149.5
Methoxymalonyl-CoA: 137.8
Isobutyryl-CoA: 112.9
Benzoyl-CoA: 104.0
Malonyl-CoA: 93.5
trans-1,2-CPDA: 82.6
2-Methylbutyryl-CoA: 82.1
Propionyl-CoA: 77.1
CHC-CoA: 68.9
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 55.5
inactive: 39.4
fatty_acid: 23.4

epoC

PKS_AT (563..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.5
inactive: 142.1
Methoxymalonyl-CoA: 75.0
Methylmalonyl-CoA: 59.4
Propionyl-CoA: 53.8
Isobutyryl-CoA: 44.7
2-Methylbutyryl-CoA: 36.5
Acetyl-CoA: 33.1
Benzoyl-CoA: 30.7
Ethylmalonyl-CoA: 30.2
CHC-CoA: 24.5
fatty_acid: 16.5
trans-1,2-CPDA: 10.8
3-Methylbutyryl-CoA: 3.9

PKS_AT (2056..2353): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.7
inactive: 137.6
Methoxymalonyl-CoA: 73.3
Methylmalonyl-CoA: 59.8
Propionyl-CoA: 46.7
Isobutyryl-CoA: 36.9
2-Methylbutyryl-CoA: 30.3
Acetyl-CoA: 29.7
Benzoyl-CoA: 28.7
Ethylmalonyl-CoA: 26.3
CHC-CoA: 20.5
fatty_acid: 11.9
trans-1,2-CPDA: 5.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (3555..3852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.2
inactive: 150.5
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 56.1
Acetyl-CoA: 35.5
Isobutyryl-CoA: 35.4
Ethylmalonyl-CoA: 31.0
Benzoyl-CoA: 27.9
2-Methylbutyryl-CoA: 23.5
CHC-CoA: 20.2
fatty_acid: 18.2
trans-1,2-CPDA: 11.9
3-Methylbutyryl-CoA: 7.8

PKS_AT (5631..5928): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 150.6
Methoxymalonyl-CoA: 141.5
Isobutyryl-CoA: 125.0
Benzoyl-CoA: 110.6
Malonyl-CoA: 101.7
2-Methylbutyryl-CoA: 93.4
trans-1,2-CPDA: 86.5
Propionyl-CoA: 83.6
Acetyl-CoA: 67.2
3-Methylbutyryl-CoA: 63.7
CHC-CoA: 62.2
inactive: 34.8
fatty_acid: 32.2

epoD

PKS_AT (561..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.6
Ethylmalonyl-CoA: 146.2
Methoxymalonyl-CoA: 134.5
Isobutyryl-CoA: 113.0
Malonyl-CoA: 97.5
Benzoyl-CoA: 97.0
2-Methylbutyryl-CoA: 97.0
Propionyl-CoA: 80.3
trans-1,2-CPDA: 70.8
3-Methylbutyryl-CoA: 67.5
CHC-CoA: 64.5
Acetyl-CoA: 59.3
inactive: 41.2
fatty_acid: 24.5

PKS_AT (2053..2350): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 177.1
Ethylmalonyl-CoA: 146.7
Methoxymalonyl-CoA: 134.3
Isobutyryl-CoA: 113.1
Malonyl-CoA: 97.7
Benzoyl-CoA: 96.2
2-Methylbutyryl-CoA: 93.7
Propionyl-CoA: 77.8
trans-1,2-CPDA: 70.6
CHC-CoA: 70.2
3-Methylbutyryl-CoA: 63.2
Acetyl-CoA: 56.0
inactive: 38.8
fatty_acid: 27.8

epoE

PKS_AT (556..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.9
inactive: 129.5
Methoxymalonyl-CoA: 65.0
Methylmalonyl-CoA: 49.5
Propionyl-CoA: 44.0
Isobutyryl-CoA: 37.8
Acetyl-CoA: 31.0
2-Methylbutyryl-CoA: 28.8
Benzoyl-CoA: 22.0
Ethylmalonyl-CoA: 17.2
fatty_acid: 15.7
CHC-CoA: 10.8
trans-1,2-CPDA: 5.2
3-Methylbutyryl-CoA: 0.0