BGC0000990 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000990 (Sorangium cellulosum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	72,852	High	epothilone	NRPS:Type I+PKS

BGC0000990 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 72,852 nt. (total: 72,852 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,853
Label		BGC0000990

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

epoA: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

epoK: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase

epoC: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

epoE: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

epoF: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

epoB: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

epoD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000990.3	1.39	NRPS, PKS	epothilone	Sorangium cellulosum
BGC0000989.3	1.17	NRPS, PKS	epothilone	Sorangium cellulosum So0157-2
BGC0000991.4	1.09	NRPS, PKS	epothilone	Sorangium cellulosum
BGC0001273.3	0.93	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.92	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.91	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.79	PKS	FR901512	fungal sp. No.14919
BGC0000988.5	0.77	NRPS, PKS	epothilone B	Sorangium cellulosum
BGC0001254.3	0.73	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000990.3	0.99	NRPS, PKS	epothilone	Sorangium cellulosum
BGC0000989.3	0.93	NRPS, PKS	epothilone	Sorangium cellulosum So0157-2
BGC0000991.4	0.89	NRPS, PKS	epothilone	Sorangium cellulosum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.76	PKS	FR901512	fungal sp. No.14919
BGC0000988.5	0.75	NRPS, PKS	epothilone B	Sorangium cellulosum
BGC0001254.3	0.74	PKS	ACT-Toxin II	Alternaria alternata
BGC0001268.4	0.70	NRPS, PKS	fusarin C	Fusarium fujikuroi

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72852:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Cys) + (Me-ccmal) + (ohmal - ohmal - redmal - Me-redmal) + (Me-ohmal - Me-Me-ccmal) + (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

epoA: mal

PKS_AT (543..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.4
inactive: 132.5
Methoxymalonyl-CoA: 59.2
Propionyl-CoA: 47.2
Methylmalonyl-CoA: 44.3
Isobutyryl-CoA: 30.9
2-Methylbutyryl-CoA: 28.1
Acetyl-CoA: 25.3
Benzoyl-CoA: 24.7
Ethylmalonyl-CoA: 18.9
CHC-CoA: 13.6
fatty_acid: 11.2
3-Methylbutyryl-CoA: 9.1
trans-1,2-CPDA: 8.4

epoB: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (528..930): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

epoC: mmal

PKS_AT (539..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.1
Ethylmalonyl-CoA: 149.5
Methoxymalonyl-CoA: 137.8
Isobutyryl-CoA: 112.9
Benzoyl-CoA: 104.0
Malonyl-CoA: 93.5
trans-1,2-CPDA: 82.6
2-Methylbutyryl-CoA: 82.1
Propionyl-CoA: 77.1
CHC-CoA: 68.9
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 55.5
inactive: 39.4
fatty_acid: 23.4

epoD: mal - mal - mal - mmal

PKS_AT (563..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.5
inactive: 145.4
Methoxymalonyl-CoA: 74.0
Methylmalonyl-CoA: 58.8
Propionyl-CoA: 53.8
Isobutyryl-CoA: 46.6
2-Methylbutyryl-CoA: 34.6
Benzoyl-CoA: 32.2
Acetyl-CoA: 31.5
Ethylmalonyl-CoA: 30.8
CHC-CoA: 25.5
fatty_acid: 17.1
trans-1,2-CPDA: 9.2
3-Methylbutyryl-CoA: 1.9

PKS_AT (2056..2353): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.0
inactive: 143.0
Methoxymalonyl-CoA: 68.9
Methylmalonyl-CoA: 53.6
Propionyl-CoA: 45.6
Isobutyryl-CoA: 35.9
Benzoyl-CoA: 29.0
2-Methylbutyryl-CoA: 27.3
Ethylmalonyl-CoA: 24.2
Acetyl-CoA: 23.4
CHC-CoA: 19.7
fatty_acid: 11.4
trans-1,2-CPDA: 5.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (3555..3852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.2
inactive: 150.5
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 56.1
Acetyl-CoA: 35.5
Isobutyryl-CoA: 35.4
Ethylmalonyl-CoA: 31.0
Benzoyl-CoA: 27.9
2-Methylbutyryl-CoA: 23.5
CHC-CoA: 20.2
fatty_acid: 18.2
trans-1,2-CPDA: 11.9
3-Methylbutyryl-CoA: 7.8

PKS_AT (5631..5928): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 150.6
Methoxymalonyl-CoA: 141.5
Isobutyryl-CoA: 125.0
Benzoyl-CoA: 110.6
Malonyl-CoA: 101.7
2-Methylbutyryl-CoA: 93.4
trans-1,2-CPDA: 86.5
Propionyl-CoA: 83.6
Acetyl-CoA: 67.2
3-Methylbutyryl-CoA: 63.7
CHC-CoA: 62.2
inactive: 34.8
fatty_acid: 32.2

epoE: mmal - mmal

PKS_AT (561..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.7
Ethylmalonyl-CoA: 150.6
Methoxymalonyl-CoA: 136.8
Isobutyryl-CoA: 115.2
Benzoyl-CoA: 99.0
Malonyl-CoA: 98.3
2-Methylbutyryl-CoA: 92.6
Propionyl-CoA: 78.8
trans-1,2-CPDA: 68.6
CHC-CoA: 66.7
3-Methylbutyryl-CoA: 64.1
Acetyl-CoA: 61.1
inactive: 42.3
fatty_acid: 26.4

PKS_AT (2053..2350): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.7
Ethylmalonyl-CoA: 150.6
Methoxymalonyl-CoA: 136.8
Isobutyryl-CoA: 115.2
Benzoyl-CoA: 99.0
Malonyl-CoA: 98.3
2-Methylbutyryl-CoA: 92.6
Propionyl-CoA: 78.8
trans-1,2-CPDA: 68.6
CHC-CoA: 66.7
3-Methylbutyryl-CoA: 64.1
Acetyl-CoA: 61.1
inactive: 42.3
fatty_acid: 26.4

epoF: mal

PKS_AT (556..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.7
inactive: 130.9
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 48.9
Propionyl-CoA: 44.0
Isobutyryl-CoA: 37.0
2-Methylbutyryl-CoA: 30.1
Acetyl-CoA: 28.6
Benzoyl-CoA: 25.0
fatty_acid: 16.9
Ethylmalonyl-CoA: 16.2
CHC-CoA: 12.4
trans-1,2-CPDA: 5.3
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor