BGC0001008 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001008 (Actinomadura madurae)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	79,708	High	maduropeptin	PKS:Iterative type I

BGC0001008 - Region 1 - NRPS,T1PKS

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Location: 1 - 79,708 nt. (total: 79,708 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 79,709
Label		BGC0001008

Tailoring functions

Oxidoreductases: Hydroxylation dehydrogenase oxidoreductase oxygenase

mdpA2: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

mdpC3: Hydroxylation

MITE: MITE0000104Hydroxylation (identity: 61.40%)

mdpC8: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

mdpD2: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

mdpE6: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

mdpE7: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

mdpH: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

mdpL: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

mdpU1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

mdpU3: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase glycosyltransferase halogenase methyltransferase transferase

mdpA1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

mdpA5: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

mdpA6: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

mdpB1: Methylation

MITE: MITE0000077Methylation (identity: 100.00%)
smcogs: SMCOG1042: O-methyltransferase

mdpC: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000028Halogenation (identity: 61.00%)
smcogs: SMCOG1119: halogenase

mdpC7: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Lyases: dehydratase

mdpA3: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

mdpB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mdpB2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

mdpC1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

mdpC5: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

mdpE: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008.5	1.65	PKS	maduropeptin	Actinomadura madurae
BGC0002341.2	1.43	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	1.35	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0001275.3	1.07	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.97	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001276.3	0.93	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002192.3	0.92	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.89	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002154.2	0.87	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.87	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008.5	0.99	PKS	maduropeptin	Actinomadura madurae
BGC0001275.3	0.78	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.78	PKS	asperlactone	Aspergillus ochraceus
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002608.2	0.75	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0000056.3	0.70	PKS	esperamicin	Actinomadura verrucosospora
BGC0001276.3	0.68	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 79708:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 45244:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 24544 - 67892:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 38373 - 79708:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mdpB: mmal

PKS_AT (548..840): mmal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 131.3
Methoxymalonyl-CoA: 115.0
Malonyl-CoA: 111.1
Ethylmalonyl-CoA: 106.3
Isobutyryl-CoA: 77.4
3-Methylbutyryl-CoA: 61.7
Propionyl-CoA: 59.9
2-Methylbutyryl-CoA: 56.2
Acetyl-CoA: 51.7
Benzoyl-CoA: 48.4
trans-1,2-CPDA: 44.2
inactive: 36.5
CHC-CoA: 35.2
fatty_acid: 19.0

mdpB2: X

AMP-binding (33..448): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPSHGVICK
Nearest Stachelhaus code(s):: meSal PLPSHGVVCK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

mdpC1: X

AMP-binding (504..910): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPCQVMVIAK
Nearest Stachelhaus code(s):: bTyr or bTyr DPAQLMLIAK (79% 8Å match)
Stachelhaus code match:: 70% (weak)

mdpE: mal

PKS_AT (618..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 70.9
Methoxymalonyl-CoA: 42.8
Methylmalonyl-CoA: 39.0
inactive: 31.6
Isobutyryl-CoA: 19.4
fatty_acid: 13.7
Ethylmalonyl-CoA: 10.6
2-Methylbutyryl-CoA: 9.8
Benzoyl-CoA: 9.0
trans-1,2-CPDA: 8.5
CHC-CoA: 3.5
Acetyl-CoA: 1.9
3-Methylbutyryl-CoA: 0.0
Propionyl-CoA: -1.0

Link to PARAS substrate predictor