BGC0001028: nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
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Location: 1 - 40,989 nt. (total: 40,989 nt).
This entry is originally from NCBI GenBank AF204805.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001028
Short description nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
Loci
AF204805.2
via Enzymatic assays
Compounds
  • nostopeptolide A2
Species Nostoc sp. GSV224 [taxonomy]
References
Chemical products information
nostopeptolide A2 Evidence:
Copy SMILES
C52H78N10O14
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF15891.2
  • nosA
 1370 - 14509 (+) nosA
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    • AAF15892.2
    • nosB
    		 14506 - 18240 (+) nosB
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      • AAF17280.1
      • nosC
      		 18312 - 28265 (+) nosC
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        • AAF17281.1
        • nosD
        		 28262 - 35614 (+) nosD
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          • AAF17282.1
          		 35820 - 36617 (+) Unknown
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            • AAF17283.1
            • nosE
            		 36665 - 37771 (+) NosE
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              • AAF17284.1
              • nosF
              		 37811 - 38632 (+) NosF
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                • AAF17285.1
                • nosG
                		 38734 - 40725 (+) NosG
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                  Biosynthesis information

                  Biosynthetic modules

                  Name
                  1
                  Type
                  nrps-type1
                  Genes
                  nosA
                  Substrates
                  isoleucine (evidence: Activity assay)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  2
                  Type
                  nrps-type1
                  Genes
                  nosA
                  Substrates
                  serine (evidence: Structure-based inference)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  3
                  Type
                  nrps-type1
                  Genes
                  nosA
                  Substrates
                  4S-methylproline (evidence: Structure-based inference)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  4
                  Type
                  nrps-type1
                  Genes
                  nosA
                  Substrates
                  leucine (evidence: Structure-based inference)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  6
                  Type
                  nrps-type1
                  Genes
                  nosC
                  Substrates
                  leucine (evidence: Activity assay)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  7
                  Type
                  nrps-type1
                  Genes
                  nosC
                  Substrates
                  glycine (evidence: Structure-based inference)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  8
                  Type
                  nrps-type1
                  Genes
                  nosC
                  Substrates
                  asparagine (evidence: Structure-based inference)
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  10
                  Type
                  nrps-type1
                  Genes
                  nosD
                  Substrates
                  proline (evidence: Structure-based inference [1], [2])
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  9
                  Type
                  nrps-type1
                  Genes
                  nosD
                  Substrates
                  tyrosine, D-tyrosine (evidence: ATP-PPi exchange assay [1], [2])
                  Integrated Monomers
                  Domains
                  condensation (LCL), adenylation
                  Name
                  5
                  Type
                  pks-modular
                  Genes
                  nosB
                  Substrates
                  Integrated Monomers
                  Domains
                  acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
                  Annotation changelog

                  Entry version: next

                  Date
                  Changes
                  Submitters
                  Reviewers
                  2024-06-14
                  MIBiG v4 annotathon
                  • (ID: KTTDKEJDYAQ7MKSNPMBDOYYX)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                  Entry version: 4

                  Date
                  Changes
                  Submitters
                  Reviewers
                  2022-10-07
                  Update chemical activity to schema version 2.11
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                  Entry version: 3

                  Date
                  Changes
                  Submitters
                  Reviewers
                  2022-09-15
                  Corrected NRP module activity
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  2022-09-15
                  Removed duplicate gene name
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  2022-09-15
                  Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  2022-09-15
                  Updated bioactivity data
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  2022-09-15
                  Updated NRP substrate specificities
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                  Entry version: 2

                  Date
                  Changes
                  Submitters
                  Reviewers
                  2019-10-16
                  Migrated from v1.4
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  2019-10-16
                  Updated compound(s) information (NPAtlas curation)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                  Entry version: 1

                  Date
                  Changes
                  Submitters
                  Reviewers
                  2015-06-12
                  Submitted
                  • (ID: N52IJH5LHPQ7BFWMKQOBW6U7)
                  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                  Detailed domain annotation
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