BGC0001047 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001047 (Pseudomonas syringae)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	22,119	syringolin A	NRPS:Type I+PKS	100%

BGC0001047 - Region 1 - NRPS,T1PKS

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Location: 1 - 22,119 nt. (total: 22,119 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 22,120
Label		BGC0001047

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001047	2.00	NRP, Polyketide	syringolin A	Pseudomonas syringae
BGC0001050	0.79	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001133	0.76	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.73	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002518	0.68	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002135	0.65	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000426	0.65	NRP	sevadicin	Paenibacillus larvae
BGC0001833	0.62	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000436	0.61	NRP	syringolin A	Rhizobium sp. AP16
BGC0002437	0.59	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001047	1.00	NRP, Polyketide	syringolin A	Pseudomonas syringae
BGC0001050	0.71	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0001133	0.70	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.69	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002135	0.66	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000426	0.66	NRP	sevadicin	Paenibacillus larvae
BGC0001833	0.65	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000436	0.64	NRP	syringolin A	Rhizobium sp. AP16
BGC0002437	0.63	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 22119:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (X - Val - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

sylC

Search NORINE for peptide: strict or relaxed

AMP-binding (750..1144): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGCVFK
Nearest Stachelhaus code(s):: Val DAFWLGCVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

sylD

Search NORINE for peptide: strict or relaxed

AMP-binding (487..899): X

nrpys: dhLys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSETGVCEK
Nearest Stachelhaus code(s):: dhLys DVSETGVCEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1566..1956): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGGQIAGVYK
Nearest Stachelhaus code(s):: Val DGGQIAGVYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (2686..2967): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 162.1
inactive: 87.7
Methoxymalonyl-CoA: 68.0
Methylmalonyl-CoA: 52.3
Propionyl-CoA: 48.3
2-Methylbutyryl-CoA: 30.9
Ethylmalonyl-CoA: 27.8
Isobutyryl-CoA: 21.2
CHC-CoA: 19.9
Benzoyl-CoA: 17.7
fatty_acid: 16.7
Acetyl-CoA: 11.6
trans-1,2-CPDA: 10.3
3-Methylbutyryl-CoA: 0.0