Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001050.1:
Cluster 1NRP / Polyketide119730

BGC0001050, thalassospiramide biosynthetic gene cluster from Tistrella bauzanensis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Thalassospiramide A
ChemSpider ID: 17214520
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Inhibitor
Molecular target: Human calpain 1 protease

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
cis-3-decenoic acid
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
ttbA (AGC65516.1)
Module 1
A specificity: None
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 2
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module is iterated
Evidence for iteration: Unknown
Module 3
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module is iterated
Evidence for iteration: Unknown
Module 5
A specificity: None
Evidence for specificity: Sequence-based prediction
C domain subtype: Other
ttbB (AGC65517.1)
Module 6
A specificity: Tyrosine
Evidence for specificity: Sequence-based prediction
C domain subtype: Unknown
Scaffold-modifying domain: Methylation
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Trans-AT type I
Starter unit:
Malonyl-CoA
Thioesterase type:
None
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
ttbA (AGC65516.1)
Module 4
Module is iterated
Evidence for iteration: Unknown

Gene cluster description

thalassospiramide (BGC0001050). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank KC181865. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?unknown
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Comments:Modules 1 and 5 are missing adenylation domains completely. Module 6 is spread over the two genes, with the condensation domain on ttbA and the remainder of the module on ttbB. The PKS portion is a trans AT system but to date no AT domain has been identified in the rest of the genome.
Contact for this cluster:Avena Ross (University of California at San Diego, Scripps Institution of Oceanography)

Literature references

1. Oh DC et al. (2007) Thalassospiramides A and B, immunosuppressive peptides from the marine bacterium Thalassospira sp. Org Lett 9(8):1525-8. doi: 10.1021/ol070294u. Epub 2007 Mar 21.
2. Ross AC et al. (2013) Biosynthetic multitasking facilitates thalassospiramide structural diversity in marine bacteria. J Am Chem Soc 135(3):1155-62. doi: 10.1021/ja3119674. Epub 2013 Jan