Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001056.1:
Cluster 1NRP / Polyketide / Other155138

BGC0001056, zeamine biosynthetic gene cluster from Serratia plymuthica. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: zeamine
SMILES string: Copy to clipboard
Molecular formula: C49H104N6O4
Exact molecular mass: 841.81913 Da ([M+H]+)
Molecular activity: Antibacterial

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Other
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Other

Nonribosomal peptide synthetases:
zmn16 (CCM44336.1)
Module B0
A specificity: Aspartate
Evidence for specificity: Structure-based inference
C domain subtype: Other
Module B1
A specificity: Histidine
Evidence for specificity: Structure-based inference
C domain subtype: Unknown
Module B2
A specificity: Asparigine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Unknown
Module B3
A specificity: Asparigine
Evidence for specificity: Structure-based inference
C domain subtype: Unknown
zmn17 (CCM44337.1)
Module B4
A specificity: Threonine
Evidence for specificity: Structure-based inference
C domain subtype: Unknown
Module B5
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Unknown
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I / PUFA synthase or related
Starter unit:
Acetyl-CoA
CCM44330.1,CCM44331.1,CCM44332.1,CCM44333.1,CCM44334.1
Thioesterase type:
None
Release / cyclization type:
Reductive release

Modular polyketide synthases:
zmn18 (CCM44338.1)
Module B6
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: A-group
Module is iterated
Evidence for iteration: Unknown

Gene cluster description

zeamine (BGC0001056). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Other. GenBank HE995400, positions 3096-53789. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Rob Lavigne (KU Leuven)

Literature references

1. Masschelein J et al. (2013 TI) A PKS/NRPS/FAS hybrid gene cluster from Serratia plymuthica RVH1 encoding the biosynthesis of three broad spectrum, zeamine-related antibiotics. PLoS One 8(1):e54143. doi: 10.1371/journal.pone.0054143. Epub 2013 Jan 17.
2. Van Houdt R et al. (2007) Characterization of a luxI/luxR-type quorum sensing system and N-acyl-homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in Serratia plymuthica RVH1. Res Microbiol 158(2):150-8. doi: 10.1016/j.resmic.2006.11.008. Epub
3. Moons P et al. (2006) Role of quorum sensing and antimicrobial component production by Serratia plymuthica in formation of biofilms, including mixed biofilms with Escherichia coli. Appl Environ Microbiol 72(11):7294-300. doi: 10.1128/AEM.01708-06. Epub
4. Masschelein J et al. (2015) The zeamine antibiotics affect the integrity of bacterial membranes. Appl Environ Microbiol 81(3):1139-46. doi: 10.1128/AEM.03146-14. Epub
5. Joleen Masschelein, Charlien Clauwers, Ufedo R. Awodi, Karen Stalmans, Wesley Vermaelen, Eveline Lescrinier, Abram Aertsen, Chris Michiels, Gregory L. Challis, Rob Lavigne. (2015) A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibiotics. Chemical Science, vol. 6, no. 2, pp. 923-929