BGC0001058: zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis
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Location: 1 - 84,773 nt. (total: 84,773 nt).
This entry is originally from NCBI GenBank EU670723.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001058
Short description zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Glycopeptide)
  • Polyketide (Other)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: EU670723.1
Compounds
  • zorbamycin
Species Streptomyces flavoviridis [taxonomy]
References
Chemical products information
zorbamycin
Copy SMILES
C55H85N19O21S2
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACG60741.1
 34 - 345 (-) hypothetical protein
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  • ACG60740.1
 395 - 799 (-) conserved hypothetical protein
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  • ACG60739.1
 851 - 2347 (-) probable DEAD-box RNA helicase
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  • ACG60738.1
 2697 - 2900 (-) putative cold shock protein
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  • ACG60737.1
 3993 - 4481 (-) small oligopeptide transporter
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  • ACG60736.1
 4849 - 5601 (+) putative thioesterase
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  • ACG60742.1
 5714 - 6343 (+) hypothetical protein
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  • ACG60743.1
 6340 - 7320 (+) putative dehydrogenase
  • Unknown
Sequence-based prediction
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  • ACG60744.1
 7317 - 8147 (+) putative hydrolase
  • Unknown
Sequence-based prediction
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  • ACG60745.1
 8223 - 10724 (+) acylase
  • Unknown
Sequence-based prediction
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  • ACG60763.1
  • zbmA
 10945 - 11343 (+) zbm binding protein
  • Resistance/immunity
Other in vivo study
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  • ACG60780.1
  • zbmVIId
 11346 - 12155 (+) acyltransferase
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60779.1
  • zbmVIIc
 12106 - 13356 (-) cytochrome P450
  • Precursor biosynthesis
Sequence-based prediction
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  • ACG60782.1
  • zbmX
 13584 - 19979 (+) NRPS(C/A/PCP/C/A/PCP)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60773.1
  • zbmIX
 19976 - 23293 (+) NRPS(C/A/PCP)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60781.1
  • zbmVIII
 23290 - 28725 (+) PKS(KS/AT/MT/KR/ACP)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60777.1
  • zbmVIIa
 28722 - 30653 (+) NRPS(C/PCP)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60776.1
  • zbmVI
 30650 - 38794 (+) NRPS(AL/ACP/C/A/PCP/C/A)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60775.1
  • zbmV
 38791 - 40584 (+) NRPS(PCP/C')
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60768.1
  • zbmG
 40581 - 41543 (+) NAD-dependent sugar epimerase
  • Precursor biosynthesis
Sequence-based prediction
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  • ACG60767.1
  • zbmF
 41536 - 43092 (+) glycosyl transferase and hydroxylase
  • Tailoring (Unknown)
Sequence-based prediction
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  • ACG60746.1
 43097 - 45010 (+) Gln-dependent amino transferase
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60772.1
  • zbmIV
 45003 - 52922 (+) NRPS(C/A/PCP/Cy/A/PCP/Cy)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60771.1
  • zbmIII
 52903 - 55446 (+) NRPS(A/PCP/Ox)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60747.1
 55443 - 56588 (+) oxygenase
  • Tailoring (Unknown)
Sequence-based prediction
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  • ACG60770.1
  • zbmII
 56585 - 57997 (+) NRPS(C)
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60748.1
 57994 - 58605 (+) Unknown
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  • ACG60766.1
  • zbmE
 58602 - 59762 (+) glycosyl transferase
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • ACG60765.1
  • zbmD
 59759 - 61387 (+) carbamoyltransferase
  • Precursor biosynthesis
Sequence-based prediction
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  • ACG60769.1
  • zbmI
 61384 - 61650 (+) type II PCP
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60764.1
  • zbmC
 61647 - 63140 (+) NTP-sugar synthase
  • Precursor biosynthesis
Sequence-based prediction
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  • ACG60749.1
 63137 - 64411 (+) SAM-dependent oxidase or methyl transferase
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACG60750.1
 64408 - 65028 (+) ankyrin-like protein
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  • ACG60783.1
  • zbmXI
 65090 - 66439 (+) NRPS(C)
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  • ACG60778.1
  • zbmVIIb
 66458 - 68233 (-) NRPS(A/PCP)
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  • ACG60751.1
 68515 - 69459 (+) alpha-ketoglutarate-dependent hydroxylase
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • ACG60752.1
 69551 - 71380 (+) NRPS(C)
  • Unknown
Sequence-based prediction
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  • ACG60753.1
 71377 - 72648 (+) type II PCP
  • Unknown
Sequence-based prediction
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  • ACG60754.1
 72645 - 72911 (+) type II PCP
  • Unknown
Sequence-based prediction
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  • ACG60755.1
 72914 - 74512 (+) acyl-CoA synthetase
  • Unknown
Sequence-based prediction
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  • ACG60756.1
 74509 - 75288 (+) NRPS(TE)
  • Unknown
Sequence-based prediction
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  • ACG60757.1
 75325 - 75933 (+) ABC transporter
  • Transport
Sequence-based prediction
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  • ACG60758.1
 75940 - 76620 (+) ABC transporter
  • Transport
Sequence-based prediction
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  • ACG60759.1
 76614 - 77213 (+) ABC transporter
  • Transport
Sequence-based prediction
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  • ACG60760.1
 77936 - 79132 (+) biotin synthase
  • Unknown
Sequence-based prediction
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  • ACG60774.1
  • zbmL
 79218 - 80231 (-) GDP-mannose-4,6-dehydratase
  • Precursor biosynthesis
Sequence-based prediction
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  • ACG60761.1
 80243 - 83659 (+) NRPS(C/A)
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  • ACG60762.1
 83596 - >84771 (-) amidohydrolase
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
zbmVI
+
zbmVIII 		Synthase subclass: Modular type I
Module 1
Genes: zbmVI
Core domains: CoA-ligase, Thiolation (ACP/PCP)
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: zbmVIII
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: L-OH
NRP-specific information
Subclass Glycopeptide
Cyclic? no
NRP-synthases
Gene Modules
zbmX
Module 4
Specificity: asparagine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 5
Specificity: histidine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: DCL
zbmIX
Module 6
Specificity: homoserine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
zbmVIIa
Module 8
Condensation domain type: LCL
zbmVI
Module 2
Specificity: serine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 3
Specificity: asparagine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: DCL
zbmV
Module 3
Condensation domain type: DCL
zbmIV
Module 9
Specificity: beta-alanine
Evidence for specificity:
  • Structure-based inference
  • Homology
Condensation domain type: LCL
Module 10
Trans-activates ZbmIII

Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
  • Homology
Condensation domain type: Heterocyclization
Modification domains: Oxidation
zbmII
Module 12
Condensation domain type: LCL
zbmIII
Module 11 [inactive]
Trans-activates cysteine

Modification domains: Oxidation
zbmXI
Module 13 [inactive]
Condensation domain type: LCL
ACG60752.1
Module 14 [inactive]
Condensation domain type: LCL
zbmVIIb
Module 8
Specificity: valine
Evidence for specificity:
  • Sequence-based prediction
Saccharide-specific information
Subclass hybrid/tailoring
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: E2T2HCM4X7QXVS4LL4JVBPPK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected NRP module numbers
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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