Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001058.1:
Cluster 1NRP / Polyketide / Saccharide184773

BGC0001058, zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: zorbamycin
PubChem ID: 70697970
SMILES string: Copy to clipboard
Molecular formula: C55H85N19O21S2
Average molecular mass: 1412.517 Da
Molecular activity: Cytotoxic
Molecular target: DNA

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Saccharide
Glycopeptide (linear)
Thioesterase type:
Type II
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
zbmX (ACG60782.1)
Module 4
A specificity: Asparigine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 5
A specificity: Histidine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
zbmIX (ACG60773.1)
Module 6
A specificity: Homoserine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
zbmVIIa (ACG60777.1)
Module 8
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
zbmVI (ACG60776.1)
Module 2
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 3
A specificity: Asparigine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
zbmV (ACG60775.1)
Module 3
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL
zbmIV (ACG60772.1)
Module 10
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 11
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
C domain subtype: Heterocyclization
Scaffold-modifying domain: Oxidation
Module 9
A specificity: Beta-alanine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
zbmII (ACG60770.1)
Module 12
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
zbmXI (ACG60783.1)
Module 13
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module is non-elongating
Evidence for non-elongation: Unknown
zbm-Orf31 (ACG60752.1)
Module 14
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module is non-elongating
Evidence for non-elongation: Unknown
zbmVIIb (ACG60778.1)
Module 8
A specificity: Valine
Evidence for specificity: Sequence-based prediction
C domain subtype: N/A
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
None
Release / cyclization type:
None

Modular polyketide synthases:
zbmVIII (ACG60781.1)
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
KR stereochemistry: B-group
Scaffold-modifying domain: Methylation

zbmVI (ACG60776.1)
Module 1
AT specificity: None
Evidence for specificity: None
Scaffold-modifying domain: None

Gene cluster description

zorbamycin (BGC0001058). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Saccharide. GenBank EU670723, positions 5714-80231. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Xiaohui Yan (The Scripps Research Institute)

Literature references

1. Wang L et al. (2007) Glycopeptide antitumor antibiotic zorbamycin from Streptomyces flavoviridis ATCC 21892: strain improvement and structure elucidation. J Nat Prod 70(3):402-6. doi: 10.1021/np060592k. Epub 2007 Feb 21.
2. Galm U et al. (2009) The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC 21892. Mol Biosyst 5(1):77-90. doi: 10.1039/b814075h. Epub 2008 Nov 12.
3. Galm U et al. (2011) Comparative analysis of the biosynthetic gene clusters and pathways for three structurally related antitumor antibiotics: bleomycin, tallysomycin, and zorbamycin. J Nat Prod 74(3):526-36. doi: 10.1021/np1008152. Epub 2011 Jan 6.
4. Huang SX et al. (2012) A designer bleomycin with significantly improved DNA cleavage activity. J Am Chem Soc 134(32):13501-9. doi: 10.1021/ja3056535. Epub 2012 Aug