Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001067.1:
Cluster 1Polyketide / Terpene135154

BGC0001067, fumagillin biosynthetic gene cluster from Aspergillus fumigatus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: fumagillin
PubChem ID: 6917655
SMILES string: Copy to clipboard
Molecular formula: C26H34O7
Average molecular mass: 458.551 Da
Molecular activity: Inhibitor
Molecular target: methionine aminopeptidase 2

Class-specific details

Biosynthetic class(es):
Polyketide / Terpene
Polyketide subclass:
Polyene (linear)
Polyketide synthase subclass:
Iterative type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
EAL85129.1
Iterative PKS subtype:
Highly reducing
5
Thioesterase type:
Other
Release / cyclization type:
Hydrolysis

Modular polyketide synthases:
fmaB, fma-PKS (EAL85129.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Activity assay
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module is iterated
Evidence for iteration: Unknown

Terpene subclass:
Other
Terpene subclass by size:
Sesquiterpene
Final isoprenoid precursor:
FPP
Terpene synthase/cyclase-encoding genes:
EAL85115.1

Gene cluster description

fumagillin (BGC0001067). Gene Cluster 1. Biosynthetic class = Polyketide/Terpene. GenBank CM000176, positions 77900-115000. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Lin HC et al. (2013) The fumagillin biosynthetic gene cluster in Aspergillus fumigatus encodes a cryptic terpene cyclase involved in the formation of beta-trans-bergamotene. J Am Chem Soc 135(12):4616-9. doi: 10.1021/ja312503y. Epub 2013 Mar
2. Lin HC et al. (2014) Generation of complexity in fungal terpene biosynthesis: discovery of a multifunctional cytochrome P450 in the fumagillin pathway. J Am Chem Soc 136(11):4426-36. doi: 10.1021/ja500881e. Epub 2014 Mar
3. Sin N et al. (1997) The anti-angiogenic agent fumagillin covalently binds and inhibits the methionine aminopeptidase, MetAP-2. Proc Natl Acad Sci U S A 94(12):6099-103.