BGC0001110: psymberin biosynthetic gene cluster from uncultured bacterium psy1
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Location: 1 - 72,054 nt. (total: 72,054 nt).
This entry is originally from NCBI GenBank FJ823461.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001110
Short description psymberin biosynthetic gene cluster from uncultured bacterium psy1
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "Psymberin harbors an isocoumarin group, hence it is cyclized and contains a lactone. However, it is not a macrolactone."
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
FJ823461.1
via Enzymatic assays
Compounds
  • psymberin
  • irciniastatin B
Species uncultured bacterium psy1 [taxonomy]
References
Chemical products information
psymberin Evidence:
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C31H47N1O11
Chemical database entries
PubCHEM
PubCHEM
irciniastatin B Evidence:
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C31H45N1O11
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ADA82578.1
 551 - 1459 (+) zinc-dependent hydrolase
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    • ADA82579.1
    		 1641 - 2921 (+) adenylosuccinate synthetase
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      • ADA82580.1
      		 3136 - 4239 (+) transposase
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        • ADA82581.1
        		 4482 - 14375 (+) trans-AT polyketide synthase
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          • ADA82582.1
          		 14379 - 15374 (-) methyltransferase
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            • ADA82583.1
            		 15375 - 16406 (-) PedK-like protein
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              • ADA82594.1
              		 17039 - 18142 (+) transposase
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                • ADA82584.1
                		 18708 - 20183 (+) transposase
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                  • ADA82585.1
                  		 20598 - 58535 (+) hybrid trans-AT polyketide synthase - nonribosomal peptide synthetase
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                    • ADA82586.1
                    		 58605 - 60320 (+) phosphoenolpyruvate synthase, beta and gamma subunit
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                      • ADA82587.1
                      		 60325 - 61818 (+) phosphoesterase
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                        • ADA82588.1
                        		 61830 - 62759 (+) phosphoenolpyruvate synthase alpha subunit
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                          • ADA82589.1
                          		 62803 - 63912 (+) acyltransferase
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                            • ADA82596.1
                            		 64017 - 65279 (+) 3-hydroxy-3-methylglutaryl-CoA synthase family
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                              • ADA82590.1
                              		 65279 - 66043 (+) crotonase superfamily
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                                • ADA82591.1
                                		 66047 - 67150 (+) flavin-dependent oxidoreductase
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                                  • ADA82592.1
                                  		 67179 - 67424 (+) acylcarrierprotein
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                                    • ADA82593.1
                                    		 67411 - 68700 (+) 3-oxoacyl ACP synthase
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                                      • ADA82595.1
                                      		 68781 - 70910 (-) transport ATPase
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                                        Biosynthesis information

                                        Biosynthetic modules

                                        Name
                                        4
                                        Type
                                        nrps-type1
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        glycine (evidence: Sequence-based prediction)
                                        Integrated Monomers
                                        Domains
                                        condensation (LCL), adenylation
                                        Name
                                        0
                                        Type
                                        pks-modular-starter
                                        Genes
                                        ADA82581.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        acyltransferase, carrier (ACP)
                                        Name
                                        1
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82581.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, carrier (ACP)
                                        Name
                                        2
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82581.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, carrier (ACP)
                                        Name
                                        3
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, methyltransferase, carrier (ACP)
                                        Name
                                        5
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, carrier (ACP)
                                        Name
                                        6
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
                                        Name
                                        7
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, dehydratase, other (Pyran synthase), carrier (ACP)
                                        Name
                                        8
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
                                        Name
                                        9
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, ketoreductase, carrier (ACP)
                                        Name
                                        10
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, methyltransferase, carrier (ACP)
                                        Name
                                        11
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, carrier (ACP)
                                        Name
                                        12
                                        Type
                                        pks-trans-at
                                        Genes
                                        ADA82585.1
                                        Substrates
                                        Integrated Monomers
                                        Domains
                                        ketosynthase, dehydratase, carrier (ACP)
                                        Annotation changelog

                                        Entry version: 4

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2022-10-07
                                        Update chemical activity to schema version 2.11
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 3

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2022-09-15
                                        Update compound structure (SMILES) for psymberin
                                        • (ID: 5UL74VURKJ25VSPPPO3H2NYB)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Linked new PubChem id for psymberin (pubchem:11692946)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Corrected NRP module activity
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Sorted modules by module number
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Removed invalid subcluster annotation
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Updated bioactivity data
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2022-09-15
                                        Updated NRP substrate specificities
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 2

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2019-10-16
                                        Migrated from v1.4
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2019-10-16
                                        Updated compound(s) information (MIBiG Annotathon)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        2019-10-16
                                        Updated compound(s) information (NPAtlas curation)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                        Entry version: 1

                                        Date
                                        Changes
                                        Submitters
                                        Reviewers
                                        2015-06-12
                                        Submitted
                                        • (ID: HQRJ2JJOIHRZHPXZ4JGWECPL)
                                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                        Detailed domain annotation
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