Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001141.1:
Cluster 1Polyketide137663

BGC0001141, brefeldin biosynthetic gene cluster from Penicillium brefeldianum. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: brefeldin A
PubChem ID: 5287620
SMILES string: Copy to clipboard
Molecular formula: C16H24O4
Average molecular mass: 280.364 Da
Molecular activity: Inhibitor
Molecular target: protein transport

Class-specific details

Biosynthetic class(es):
Polyketide
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Iterative type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
AIA58899
Iterative PKS subtype:
Highly reducing
7
Thioesterase type:
Other
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
Bref-PKS (AIA58899)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Activity assay
KR stereochemistry: Unknown
Scaffold-modifying domain: None

Gene cluster description

brefeldin (BGC0001141). Gene Cluster 1. Biosynthetic class = Polyketide. GenBank KJ728786, positions 3000-24000. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Zabala AO et al. (2014) Fungal polyketide synthase product chain-length control by partnering thiohydrolase. ACS Chem Biol 9(7):1576-86. doi: 10.1021/cb500284t. Epub 2014 May
2. Misumi Y et al. (1986) Novel blockade by brefeldin A of intracellular transport of secretory proteins in cultured rat hepatocytes. J Biol Chem 261(24):11398-403.
3. Fujiwara T et al. (1988) Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem 263(34):18545-52.