BGC0001194 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001194 (Embleya scabrispora)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	72,530	High	hitachimycin	PKS

BGC0001194 - Region 1 - T1PKS

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Location: 1 - 72,530 nt. (total: 72,530 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,531
Label		BGC0001194

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

hitM1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

hitM3: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

hitM4: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

hitC: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase

hitF: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Ligases: ligase synthase

hitB: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

hitE: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

hitP1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hitP2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hitP3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hitP4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hitP5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194.5	0.99	PKS	hitachimycin	Streptomyces scabrisporus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001194.5	0.99	PKS	hitachimycin	Streptomyces scabrisporus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.66	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72530:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - redmal) + (ohmal - ccmal) + (ohmal) + (ccmal - pk - ccmal) + (ccmal)
Non-colinear ordering:: hitP1 + hitP2 + hitP5 + hitP4 + hitP3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

hitP1: mal - mal

PKS_AT (670..960): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 120.5
Methoxymalonyl-CoA: 65.2
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 46.1
2-Methylbutyryl-CoA: 22.9
trans-1,2-CPDA: 16.9
CHC-CoA: 15.6
Ethylmalonyl-CoA: 15.2
Isobutyryl-CoA: 12.7
Acetyl-CoA: 11.4
Benzoyl-CoA: 10.1
fatty_acid: 7.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2392..2683): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 122.4
Methoxymalonyl-CoA: 57.5
Methylmalonyl-CoA: 47.0
Propionyl-CoA: 31.9
Ethylmalonyl-CoA: 27.0
Acetyl-CoA: 21.1
Benzoyl-CoA: 19.9
Isobutyryl-CoA: 19.8
2-Methylbutyryl-CoA: 17.7
CHC-CoA: 14.8
fatty_acid: 11.7
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

hitB: X

AMP-binding (26..435): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFFHLTGACK
Nearest Stachelhaus code(s):: dclohBfa or Hpg DIFHLGLLCK (41% 8Å match); Hpg DIFHLGLLCK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

hitC: mal

PKS_AT (80..283): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 10.2
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

hitE: X

AMP-binding (19..416): X

nrpys: bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (82% 8Å match); Ala DCFFFALITK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

hitP2: mal - mal

PKS_AT (557..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.0
inactive: 107.5
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 62.8
Propionyl-CoA: 47.0
Ethylmalonyl-CoA: 35.6
Isobutyryl-CoA: 30.1
2-Methylbutyryl-CoA: 28.7
Benzoyl-CoA: 26.2
Acetyl-CoA: 26.1
CHC-CoA: 23.7
fatty_acid: 16.7
trans-1,2-CPDA: 16.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2088..2375): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.3
inactive: 97.6
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 45.2
Propionyl-CoA: 35.4
Benzoyl-CoA: 24.1
Ethylmalonyl-CoA: 23.2
Isobutyryl-CoA: 22.4
2-Methylbutyryl-CoA: 20.3
Acetyl-CoA: 19.2
fatty_acid: 16.2
CHC-CoA: 10.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

hitP3: mal

PKS_AT (549..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.7
inactive: 103.7
Methoxymalonyl-CoA: 57.1
Methylmalonyl-CoA: 54.7
Propionyl-CoA: 41.4
Benzoyl-CoA: 26.3
Ethylmalonyl-CoA: 24.8
2-Methylbutyryl-CoA: 24.3
Isobutyryl-CoA: 22.3
Acetyl-CoA: 18.7
fatty_acid: 14.0
CHC-CoA: 12.5
trans-1,2-CPDA: 8.6
3-Methylbutyryl-CoA: 0.0

hitP4: mal - pk - mal

PKS_AT (574..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 104.6
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 46.2
Propionyl-CoA: 34.1
Ethylmalonyl-CoA: 25.4
Isobutyryl-CoA: 24.1
2-Methylbutyryl-CoA: 22.9
Benzoyl-CoA: 22.4
fatty_acid: 22.3
Acetyl-CoA: 20.3
CHC-CoA: 15.2
trans-1,2-CPDA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2254..2551): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.3
Methylmalonyl-CoA: 212.2
Methoxymalonyl-CoA: 170.9
2-Methylbutyryl-CoA: 142.9
trans-1,2-CPDA: 138.3
Propionyl-CoA: 109.5
CHC-CoA: 95.1
Malonyl-CoA: 94.7
Benzoyl-CoA: 80.7
Isobutyryl-CoA: 74.0
Acetyl-CoA: 52.4
3-Methylbutyryl-CoA: 41.7
fatty_acid: 39.4
inactive: 23.4

PKS_AT (3946..4233): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.6
inactive: 104.5
Methoxymalonyl-CoA: 50.9
Methylmalonyl-CoA: 46.4
Propionyl-CoA: 35.2
Isobutyryl-CoA: 26.6
Ethylmalonyl-CoA: 25.1
2-Methylbutyryl-CoA: 24.4
Benzoyl-CoA: 23.6
fatty_acid: 23.2
Acetyl-CoA: 19.9
CHC-CoA: 13.9
trans-1,2-CPDA: 9.3
3-Methylbutyryl-CoA: 0.0

hitP5: mal

PKS_AT (556..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.3
inactive: 89.0
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 52.3
Propionyl-CoA: 34.4
Isobutyryl-CoA: 31.1
Ethylmalonyl-CoA: 28.1
Benzoyl-CoA: 23.6
Acetyl-CoA: 20.2
trans-1,2-CPDA: 14.1
fatty_acid: 13.8
CHC-CoA: 9.6
2-Methylbutyryl-CoA: 0.3
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor