BGC0001199 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001199 (Streptomyces sp. NBRC 109706)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	70,749	High	akaeolide	PKS

BGC0001199 - Region 1 - T1PKS

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Location: 1 - 70,749 nt. (total: 70,749 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,750
Label		BGC0001199

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ctg1_orf525: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ctg1_orf528: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ctg1_orf533: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ctg1_orf535: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ctg1_orf536: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: transferase

ctg1_orf518: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: esterase

ctg1_orf515: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

ctg1_orf521: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf522: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf523: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ctg1_orf524: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf534: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001199.5	1.00	PKS	akaeolide	Streptomyces sp. NBRC 109706
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001199.5	1.00	PKS	akaeolide	Streptomyces sp. NBRC 109706
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 70749:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - Me-redmal) + (ccmal - Me-ccmal) + (mal - pk) + (mal)
Non-colinear ordering:: ctg1_orf521 + ctg1_orf522 + ctg1_orf523 + ctg1_orf524

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_orf521: mal - mmal - mmal

PKS_AT (548..841): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 168.5
inactive: 89.6
Methylmalonyl-CoA: 56.5
Methoxymalonyl-CoA: 49.1
Isobutyryl-CoA: 30.2
Ethylmalonyl-CoA: 26.6
Propionyl-CoA: 22.9
Benzoyl-CoA: 18.8
fatty_acid: 16.0
Acetyl-CoA: 15.0
trans-1,2-CPDA: 7.5
CHC-CoA: 5.1
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1556..1855): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.7
Ethylmalonyl-CoA: 190.6
Methoxymalonyl-CoA: 162.9
trans-1,2-CPDA: 118.4
2-Methylbutyryl-CoA: 118.2
Propionyl-CoA: 93.4
Malonyl-CoA: 91.6
CHC-CoA: 90.2
Isobutyryl-CoA: 73.6
Benzoyl-CoA: 66.8
Acetyl-CoA: 54.9
fatty_acid: 41.0
3-Methylbutyryl-CoA: 36.4
inactive: 27.1

PKS_AT (3304..3603): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.6
Ethylmalonyl-CoA: 189.2
Methoxymalonyl-CoA: 166.6
trans-1,2-CPDA: 118.6
2-Methylbutyryl-CoA: 116.8
Malonyl-CoA: 98.3
Propionyl-CoA: 93.4
CHC-CoA: 87.7
Isobutyryl-CoA: 76.1
Benzoyl-CoA: 72.4
Acetyl-CoA: 50.6
fatty_acid: 41.0
3-Methylbutyryl-CoA: 37.9
inactive: 28.2

ctg1_orf522: mal - mmal

PKS_AT (561..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 165.7
inactive: 87.3
Methylmalonyl-CoA: 53.9
Methoxymalonyl-CoA: 45.3
Benzoyl-CoA: 27.7
Isobutyryl-CoA: 26.0
Propionyl-CoA: 20.1
Ethylmalonyl-CoA: 19.4
Acetyl-CoA: 13.3
fatty_acid: 11.1
CHC-CoA: 7.9
trans-1,2-CPDA: 6.2
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (2304..2603): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.6
Ethylmalonyl-CoA: 189.2
Methoxymalonyl-CoA: 166.6
trans-1,2-CPDA: 118.6
2-Methylbutyryl-CoA: 116.8
Malonyl-CoA: 98.3
Propionyl-CoA: 93.4
CHC-CoA: 87.7
Isobutyryl-CoA: 76.1
Benzoyl-CoA: 72.4
Acetyl-CoA: 50.6
fatty_acid: 41.0
3-Methylbutyryl-CoA: 37.9
inactive: 28.2

ctg1_orf523: mal - pk

PKS_AT (569..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.8
inactive: 79.2
Methylmalonyl-CoA: 48.7
Methoxymalonyl-CoA: 44.5
Isobutyryl-CoA: 26.3
Ethylmalonyl-CoA: 22.7
Benzoyl-CoA: 17.4
Propionyl-CoA: 17.3
Acetyl-CoA: 15.8
fatty_acid: 15.7
CHC-CoA: 11.9
trans-1,2-CPDA: 7.7
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1597..1896): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 227.2
Methylmalonyl-CoA: 208.1
Methoxymalonyl-CoA: 174.7
2-Methylbutyryl-CoA: 167.9
trans-1,2-CPDA: 152.2
CHC-CoA: 122.0
Propionyl-CoA: 116.5
Malonyl-CoA: 102.4
Isobutyryl-CoA: 92.0
Benzoyl-CoA: 72.7
Acetyl-CoA: 59.3
3-Methylbutyryl-CoA: 42.0
inactive: 36.9
fatty_acid: 27.2

ctg1_orf524: mal

PKS_AT (561..855): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.7
inactive: 80.8
Methylmalonyl-CoA: 49.4
Methoxymalonyl-CoA: 42.0
Isobutyryl-CoA: 30.0
Propionyl-CoA: 21.6
Ethylmalonyl-CoA: 20.0
Acetyl-CoA: 18.6
Benzoyl-CoA: 16.4
fatty_acid: 15.6
CHC-CoA: 13.5
trans-1,2-CPDA: 13.2
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor