BGC0001287 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001287 (Streptomyces leeuwenhoekii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like	1	94,879	High	chaxamycin A/chaxamycin B/chaxamycin C/chaxamycin D	PKS

BGC0001287 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 94,879 nt. (total: 94,879 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 94,880
Label		BGC0001287

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

cxm18: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

cxm19: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

cxm22: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

cxm23: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

cxm4: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

cxmL: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

cxmS: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

cxmT: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: acylase aminotransferase methyltransferase

cxm24: Methylation

MITE: MITE0000085Methylation (identity: 65.40%)
smcogs: SMCOG1042: O-methyltransferase

cxmB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

cxmD: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

cxmK: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase hydrolase

cxmM: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

cxmR: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

cxmA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cxmE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cxmG: 3-dehydroquinate synthase

smcogs: SMCOG1183: 3-dehydroquinate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001287.5	1.44	PKS	chaxamycin A, chaxamycin B, chaxamycin C, chaxamycin D	Streptomyces leeuwenhoekii
BGC0001273.3	0.93	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.92	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.89	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.58	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.55	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.48	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0002099.3	0.47	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0000018.5	0.42	PKS	angolamycin	Streptomyces eurythermus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001287.5	1.00	PKS	chaxamycin A, chaxamycin B, chaxamycin C, chaxamycin D	Streptomyces leeuwenhoekii
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000067.5	0.67	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 515 - 94879:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (AHBA - Me-ccmal - mal - Me-ohmal) + (Me-ccmal - Me-ccmal - Me-ccmal) + (Me-ccmal) + (Me-ccmal) + (ccmal - ccmal)
Non-colinear ordering:: cxmA + cxmB + cxmC + cxmD + cxmE

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cxmE: mal - mal

PKS_AT (564..859): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.3
inactive: 117.8
Methoxymalonyl-CoA: 74.4
Methylmalonyl-CoA: 64.7
Propionyl-CoA: 40.1
Ethylmalonyl-CoA: 29.9
2-Methylbutyryl-CoA: 29.6
Isobutyryl-CoA: 28.7
fatty_acid: 25.7
trans-1,2-CPDA: 24.8
CHC-CoA: 23.6
Benzoyl-CoA: 21.9
Acetyl-CoA: 14.1
3-Methylbutyryl-CoA: 11.8

PKS_AT (2283..2578): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.3
inactive: 117.8
Methoxymalonyl-CoA: 74.4
Methylmalonyl-CoA: 64.7
Propionyl-CoA: 40.1
Ethylmalonyl-CoA: 29.9
2-Methylbutyryl-CoA: 29.6
Isobutyryl-CoA: 28.7
fatty_acid: 25.7
trans-1,2-CPDA: 24.8
CHC-CoA: 23.6
Benzoyl-CoA: 21.9
Acetyl-CoA: 14.1
3-Methylbutyryl-CoA: 11.8

cxmD: mmal

PKS_AT (565..863): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.5
Ethylmalonyl-CoA: 199.3
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 125.2
trans-1,2-CPDA: 115.5
Propionyl-CoA: 96.1
Malonyl-CoA: 87.8
CHC-CoA: 79.3
Isobutyryl-CoA: 74.3
Benzoyl-CoA: 62.3
Acetyl-CoA: 45.0
fatty_acid: 32.1
inactive: 13.3
3-Methylbutyryl-CoA: 11.0

cxmC: mmal

PKS_AT (564..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.6
Ethylmalonyl-CoA: 199.8
Methoxymalonyl-CoA: 171.3
2-Methylbutyryl-CoA: 131.5
trans-1,2-CPDA: 116.1
Propionyl-CoA: 107.8
Malonyl-CoA: 102.2
CHC-CoA: 88.0
Isobutyryl-CoA: 76.9
Benzoyl-CoA: 66.9
Acetyl-CoA: 51.8
fatty_acid: 35.7
inactive: 21.3
3-Methylbutyryl-CoA: 17.1

cxmB: mmal - mmal - mmal

PKS_AT (566..869): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.3
Ethylmalonyl-CoA: 212.0
Methoxymalonyl-CoA: 176.1
2-Methylbutyryl-CoA: 134.6
trans-1,2-CPDA: 120.1
Propionyl-CoA: 105.0
Malonyl-CoA: 104.5
CHC-CoA: 93.2
Isobutyryl-CoA: 82.2
Benzoyl-CoA: 64.4
Acetyl-CoA: 54.7
fatty_acid: 41.5
inactive: 19.1
3-Methylbutyryl-CoA: 16.6

PKS_AT (2300..2595): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.5
Ethylmalonyl-CoA: 202.6
Methoxymalonyl-CoA: 170.8
2-Methylbutyryl-CoA: 129.1
trans-1,2-CPDA: 116.8
Propionyl-CoA: 101.2
Malonyl-CoA: 97.2
CHC-CoA: 89.9
Isobutyryl-CoA: 75.7
Benzoyl-CoA: 55.9
Acetyl-CoA: 46.4
fatty_acid: 33.0
3-Methylbutyryl-CoA: 21.5
inactive: 18.0

PKS_AT (3981..4278): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.5
Ethylmalonyl-CoA: 200.4
Methoxymalonyl-CoA: 171.4
2-Methylbutyryl-CoA: 125.8
trans-1,2-CPDA: 124.3
Propionyl-CoA: 103.6
Malonyl-CoA: 97.7
CHC-CoA: 87.9
Isobutyryl-CoA: 80.4
Benzoyl-CoA: 66.1
Acetyl-CoA: 47.2
fatty_acid: 38.7
inactive: 27.0
3-Methylbutyryl-CoA: 15.7

cxmA: AHBA - mmal - mal - mmal

CAL_domain (33..437): AHBA

minowa: AHBA

Prediction, score:

AHBA: 461.1
shikimic_acid: 275.5
Acetyl-CoA: 15.3
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1614..1906): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.9
Ethylmalonyl-CoA: 206.3
Methoxymalonyl-CoA: 167.6
2-Methylbutyryl-CoA: 126.3
trans-1,2-CPDA: 118.3
Propionyl-CoA: 105.8
Malonyl-CoA: 101.3
CHC-CoA: 83.8
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 69.1
Acetyl-CoA: 52.0
fatty_acid: 27.1
inactive: 27.0
3-Methylbutyryl-CoA: 20.9

PKS_AT (3292..3590): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.1
inactive: 98.8
Methoxymalonyl-CoA: 77.2
Methylmalonyl-CoA: 64.8
Isobutyryl-CoA: 45.3
Propionyl-CoA: 38.3
Ethylmalonyl-CoA: 29.8
Benzoyl-CoA: 26.6
CHC-CoA: 24.4
Acetyl-CoA: 22.1
fatty_acid: 11.1
2-Methylbutyryl-CoA: 11.1
trans-1,2-CPDA: 10.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (4557..4849): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.4
Ethylmalonyl-CoA: 199.5
Methoxymalonyl-CoA: 166.6
2-Methylbutyryl-CoA: 120.5
trans-1,2-CPDA: 112.7
Propionyl-CoA: 95.5
Malonyl-CoA: 86.3
CHC-CoA: 82.1
Isobutyryl-CoA: 70.1
Benzoyl-CoA: 59.8
Acetyl-CoA: 43.6
fatty_acid: 35.0
inactive: 12.4
3-Methylbutyryl-CoA: 9.9

Link to PARAS substrate predictor