BGC0001288 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001288 (Micromonospora sp. GMKU326)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,PKS-like	1	149,333	maklamicin	PKS	100%

BGC0001288 - Region 1 - PKS-like,T1PKS

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Location: 1 - 149,333 nt. (total: 149,333 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 149,334
Label		BGC0001288

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001288	1.74	Polyketide	maklamicin	Micromonospora sp. GMKU326
BGC0000844	1.42	Other	clavulanic acid	Streptomyces clavuligerus
BGC0000016	1.34	Other	amphotericin B	Streptomyces nodosus
BGC0002615	1.12	Terpene	4-oxomacrophorin A, macrophorin A, 5'-epimacrophorin B	Penicillium terrestre
BGC0000170	1.12	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0002514	1.08	Polyketide	julichrome Q6.6	Streptomyces sampsonii
BGC0001276	1.05	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001529	1.04	Alkaloid	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0000041	1.04	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000160	1.02	Polyketide	terreic acid	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001288	1.00	Polyketide	maklamicin	Micromonospora sp. GMKU326
BGC0001276	0.68	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0000844	0.68	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001273	0.68	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275	0.68	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244	0.67	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0000120	0.67	Polyketide	patulin	Penicillium expansum
BGC0000016	0.67	Other	amphotericin B	Streptomyces nodosus
BGC0000160	0.67	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0001137	0.66	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 149333:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (Me-ccmal - ccmal - ccmal - Me-ccmal) + (Me-redmal - Me-ccmal) + (mal) + (Me-ccmal - ccmal - ccmal - Me-redmal) + (redmal - Me-ccmal) + (pk)
Non-colinear ordering:: makA1 + BAQ25481.1 + BAQ25482.1 + makA4 + makA2 + makA3 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 64146:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal - ccmal - Me-ccmal) + (Me-redmal - Me-ccmal) + (mal)
Non-colinear ordering:: BAQ25481.1 + BAQ25482.1 + makA4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 31468 - 72506:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - Me-ccmal) + (mal) + (pk)
Non-colinear ordering:: BAQ25482.1 + makA4 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 48259 - 89300:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (pk)
Non-colinear ordering:: makA1 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 49341 - 149333:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - Me-ccmal - Me-ccmal - ccmal) + (Me-ccmal - ccmal - ccmal - Me-redmal) + (redmal - Me-ccmal) + (pk)
Non-colinear ordering:: makA1 + makA2 + makA3 + BAQ25507.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BAQ25481.1

PKS_AT (658..953): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 166.8
Methoxymalonyl-CoA: 136.4
Ethylmalonyl-CoA: 127.5
2-Methylbutyryl-CoA: 108.4
trans-1,2-CPDA: 105.0
CHC-CoA: 99.2
Propionyl-CoA: 96.3
Malonyl-CoA: 88.6
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 55.2
fatty_acid: 53.4
3-Methylbutyryl-CoA: 38.7
Acetyl-CoA: 32.8
inactive: 16.6

PKS_AT (2439..2729): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.5
inactive: 91.3
Methoxymalonyl-CoA: 55.1
Methylmalonyl-CoA: 45.0
Propionyl-CoA: 28.4
Ethylmalonyl-CoA: 22.7
fatty_acid: 20.3
Isobutyryl-CoA: 19.3
Acetyl-CoA: 18.0
Benzoyl-CoA: 11.9
CHC-CoA: 9.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4159..4446): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.6
inactive: 83.9
Methoxymalonyl-CoA: 53.6
Methylmalonyl-CoA: 40.9
Propionyl-CoA: 28.9
Ethylmalonyl-CoA: 22.2
fatty_acid: 18.7
Isobutyryl-CoA: 17.9
Acetyl-CoA: 17.6
Benzoyl-CoA: 9.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (5883..6226): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 195.5
Ethylmalonyl-CoA: 187.7
Methoxymalonyl-CoA: 169.0
2-Methylbutyryl-CoA: 141.2
Propionyl-CoA: 125.2
trans-1,2-CPDA: 113.5
CHC-CoA: 105.5
Malonyl-CoA: 98.3
Isobutyryl-CoA: 64.9
Benzoyl-CoA: 49.7
inactive: 30.9
fatty_acid: 30.8
3-Methylbutyryl-CoA: 29.4
Acetyl-CoA: 23.2

BAQ25482.1

PKS_AT (582..920): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.7
Ethylmalonyl-CoA: 174.6
Methoxymalonyl-CoA: 165.0
2-Methylbutyryl-CoA: 137.5
Propionyl-CoA: 121.2
trans-1,2-CPDA: 118.4
CHC-CoA: 109.7
Malonyl-CoA: 97.0
Isobutyryl-CoA: 58.2
Benzoyl-CoA: 50.1
inactive: 32.6
fatty_acid: 31.6
3-Methylbutyryl-CoA: 23.6
Acetyl-CoA: 21.3

PKS_AT (2673..3018): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.3
Ethylmalonyl-CoA: 183.5
Methoxymalonyl-CoA: 166.8
2-Methylbutyryl-CoA: 135.4
Propionyl-CoA: 120.9
trans-1,2-CPDA: 117.7
CHC-CoA: 113.8
Malonyl-CoA: 99.9
Isobutyryl-CoA: 61.7
Benzoyl-CoA: 51.5
inactive: 33.4
fatty_acid: 32.1
Acetyl-CoA: 25.4
3-Methylbutyryl-CoA: 24.7

makA4

PKS_AT (572..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.2
inactive: 83.2
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 48.0
Propionyl-CoA: 34.1
Acetyl-CoA: 21.0
Isobutyryl-CoA: 17.9
fatty_acid: 17.7
Ethylmalonyl-CoA: 15.0
Benzoyl-CoA: 14.3
2-Methylbutyryl-CoA: 11.7
CHC-CoA: 10.2
trans-1,2-CPDA: 8.3
3-Methylbutyryl-CoA: 0.0

BAQ25507.1

PKS_AT (543..833): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 126.1
Methoxymalonyl-CoA: 100.3
Ethylmalonyl-CoA: 98.3
Malonyl-CoA: 73.7
trans-1,2-CPDA: 64.1
Isobutyryl-CoA: 63.8
Propionyl-CoA: 62.7
2-Methylbutyryl-CoA: 51.2
Acetyl-CoA: 42.7
Benzoyl-CoA: 38.3
fatty_acid: 37.7
3-Methylbutyryl-CoA: 34.8
CHC-CoA: 26.4
inactive: 11.7

makA1

PKS_AT (575..871): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.4
inactive: 112.5
Methoxymalonyl-CoA: 64.0
Methylmalonyl-CoA: 55.0
Propionyl-CoA: 33.0
Isobutyryl-CoA: 27.4
Acetyl-CoA: 25.4
Benzoyl-CoA: 19.5
trans-1,2-CPDA: 12.3
CHC-CoA: 11.8
fatty_acid: 8.0
2-Methylbutyryl-CoA: 7.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.5

PKS_AT (1613..1960): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.1
Ethylmalonyl-CoA: 177.8
Methoxymalonyl-CoA: 165.2
2-Methylbutyryl-CoA: 133.4
Propionyl-CoA: 117.4
CHC-CoA: 110.4
trans-1,2-CPDA: 108.8
Malonyl-CoA: 96.0
Isobutyryl-CoA: 60.4
Benzoyl-CoA: 51.8
inactive: 35.1
fatty_acid: 33.6
3-Methylbutyryl-CoA: 24.8
Acetyl-CoA: 23.0

PKS_AT (3387..3677): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.4
inactive: 85.4
Methoxymalonyl-CoA: 59.3
Methylmalonyl-CoA: 48.8
Propionyl-CoA: 26.6
Isobutyryl-CoA: 21.4
Acetyl-CoA: 14.8
fatty_acid: 10.9
Ethylmalonyl-CoA: 8.6
Benzoyl-CoA: 8.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (5170..5513): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.2
Ethylmalonyl-CoA: 174.3
Methoxymalonyl-CoA: 160.0
2-Methylbutyryl-CoA: 133.2
Propionyl-CoA: 119.9
CHC-CoA: 111.6
trans-1,2-CPDA: 109.7
Malonyl-CoA: 96.8
Isobutyryl-CoA: 56.4
Benzoyl-CoA: 47.6
inactive: 35.6
fatty_acid: 34.9
Acetyl-CoA: 21.4
3-Methylbutyryl-CoA: 18.8

PKS_AT (6930..7273): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.2
Ethylmalonyl-CoA: 181.3
Methoxymalonyl-CoA: 160.7
2-Methylbutyryl-CoA: 134.8
Propionyl-CoA: 121.5
trans-1,2-CPDA: 110.6
CHC-CoA: 110.2
Malonyl-CoA: 100.2
Isobutyryl-CoA: 59.5
Benzoyl-CoA: 47.9
inactive: 31.4
fatty_acid: 28.7
Acetyl-CoA: 24.1
3-Methylbutyryl-CoA: 22.9

PKS_AT (8702..8992): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.8
inactive: 90.2
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 42.6
Propionyl-CoA: 27.2
Isobutyryl-CoA: 21.5
Ethylmalonyl-CoA: 18.1
fatty_acid: 17.6
Acetyl-CoA: 14.8
Benzoyl-CoA: 9.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

makA2

PKS_AT (635..930): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.5
Methoxymalonyl-CoA: 135.6
Ethylmalonyl-CoA: 128.1
2-Methylbutyryl-CoA: 109.5
trans-1,2-CPDA: 106.0
CHC-CoA: 100.5
Propionyl-CoA: 97.0
Malonyl-CoA: 84.8
Isobutyryl-CoA: 78.6
Benzoyl-CoA: 55.4
fatty_acid: 53.4
3-Methylbutyryl-CoA: 38.9
Acetyl-CoA: 32.8
inactive: 16.5

PKS_AT (2355..2638): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.3
inactive: 86.3
Methoxymalonyl-CoA: 56.9
Methylmalonyl-CoA: 41.3
Propionyl-CoA: 25.1
Ethylmalonyl-CoA: 18.6
Acetyl-CoA: 18.6
Isobutyryl-CoA: 14.1
Benzoyl-CoA: 9.4
trans-1,2-CPDA: 0.0
fatty_acid: -0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4087..4377): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.4
inactive: 88.6
Methoxymalonyl-CoA: 54.9
Methylmalonyl-CoA: 44.8
Propionyl-CoA: 20.9
fatty_acid: 18.9
Acetyl-CoA: 18.7
Isobutyryl-CoA: 17.0
Ethylmalonyl-CoA: 15.8
Benzoyl-CoA: 10.8
2-Methylbutyryl-CoA: 1.0
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5841..6133): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 190.9
Ethylmalonyl-CoA: 178.2
Methoxymalonyl-CoA: 161.1
2-Methylbutyryl-CoA: 133.1
Propionyl-CoA: 121.3
CHC-CoA: 109.8
trans-1,2-CPDA: 108.2
Malonyl-CoA: 97.1
Isobutyryl-CoA: 57.8
Benzoyl-CoA: 51.4
inactive: 32.2
fatty_acid: 28.9
3-Methylbutyryl-CoA: 27.3
Acetyl-CoA: 25.6

makA3

PKS_AT (586..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.8
inactive: 83.1
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 44.3
Ethylmalonyl-CoA: 28.1
Propionyl-CoA: 26.8
Acetyl-CoA: 18.6
Isobutyryl-CoA: 15.4
Benzoyl-CoA: 13.7
2-Methylbutyryl-CoA: 9.5
trans-1,2-CPDA: 8.9
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
fatty_acid: -2.1

PKS_AT (2646..2940): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.4
Ethylmalonyl-CoA: 180.7
Methoxymalonyl-CoA: 163.8
2-Methylbutyryl-CoA: 136.6
Propionyl-CoA: 121.7
CHC-CoA: 107.7
trans-1,2-CPDA: 107.5
Malonyl-CoA: 98.8
Isobutyryl-CoA: 58.3
Benzoyl-CoA: 51.9
inactive: 32.3
3-Methylbutyryl-CoA: 28.1
fatty_acid: 27.5
Acetyl-CoA: 24.5

makD1

AMP-binding (10..370): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --LFVGRITK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)