BGC0001297 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001297 (Streptomyces calvus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS-like,HR-T2PKS,NRPS	1	55,539	WS9326A/WS9326C/WS9326D	NRPS:Type I	100%

BGC0001297 - Region 1 - HR-T2PKS,NRPS,NRPS-like

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Location: 1 - 55,539 nt. (total: 55,539 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,540
Label		BGC0001297

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001297	2.84	NRP	WS9326	Streptomyces calvus
BGC0002377	1.93	NRP	WS9326B, WS9326A, WS9326G, WS9326F	Streptomyces sp. SNM55
BGC0001133	1.58	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000426	1.41	NRP	sevadicin	Paenibacillus larvae
BGC0001185	1.41	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0001925	1.34	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0002075	1.33	NRP, Alkaloid	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001890	1.33	NRP	amphi-enterobactin 1, amphi-enterobactin 2, amphi-enterobactin 3, amphi-enterobactin 4	Vibrio campbellii ATCC BAA-1116
BGC0000375	1.33	NRP	indigoidine	Streptomyces chromofuscus
BGC0002688	1.33	NRP	benarthin, dibenarthin	Planobispora rosea

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001297	0.99	NRP	WS9326	Streptomyces calvus
BGC0002377	0.84	NRP	WS9326B, WS9326A, WS9326G, WS9326F	Streptomyces sp. SNM55
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000416	0.70	NRP	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0001185	0.70	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0001434	0.70	NRP	nematophin	Xenorhabdus budapestensis
BGC0002017	0.69	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000417	0.69	NRP	rhodochelin	Rhodococcus jostii RHA1
BGC0002688	0.68	NRP	benarthin, dibenarthin	Planobispora rosea
BGC0001132	0.68	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 55539:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - NMe-X) + (Val - D-Phe) + (Ser) + (aThr) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 40478:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - NMe-X) + (Val - D-Phe) + (Ser) + (aThr) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 0 - 37498:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - NMe-X) + (Val - D-Phe) + (Ser) + (aThr) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 0 - 29145:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - D-Phe) + (Ser) + (aThr) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 2136 - 55539:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - NMe-X) + (Val - D-Phe) + (Ser) + (aThr) + (Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cal23

Search NORINE for peptide: strict or relaxed

AMP-binding (21..419): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cal22

Search NORINE for peptide: strict or relaxed

AMP-binding (390..788): aThr

nrpys: aThr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code(s):: aThr DFWSVGMVHK (100% 8Å match); Thr DFWSVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

cal19

Search NORINE for peptide: strict or relaxed

AMP-binding (1043..1451): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLVDK
Nearest Stachelhaus code(s):: Ser DVWHLSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cal18

Search NORINE for peptide: strict or relaxed

AMP-binding (470..844): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAYLWGAVMK
Nearest Stachelhaus code(s):: Val DAYLWGAVMK (100% 8Å match); Leu DAYLWGAVMK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1488..1886): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTVAAVCK
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cal17

Search NORINE for peptide: strict or relaxed

AMP-binding (470..892): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1558..1958): X

nrpys: Phe, Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTTAAVCK
Nearest Stachelhaus code(s):: Phe or Tyr DASTTAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)