BGC0001303 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001303 (Micromonospora sp. RL09-050-HVF-A)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	108,672	salinilactam	Polyketide	84%

BGC0001303 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 108,672 nt. (total: 108,672 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 108,673
Label		BGC0001303

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001303.1	0.87	Polyketide	lobosamide A, lobosamide B, lobosamide C	Micromonospora sp. RL09-050-HVF-A
BGC0000142.1	0.39	Polyketide	salinilactam	Salinispora tropica CNB-440
BGC0001164.1	0.33	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067.1	0.32	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002031.1	0.32	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001276.1	0.31	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.1	0.31	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001343.1	0.31	Polyketide	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15
BGC0001244.1	0.31	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.31	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001303.1	0.96	Polyketide	lobosamide A, lobosamide B, lobosamide C	Micromonospora sp. RL09-050-HVF-A
BGC0000142.1	0.71	Polyketide	salinilactam	Salinispora tropica CNB-440
BGC0001164.1	0.66	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067.1	0.66	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0002031.1	0.66	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001276.1	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.1	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001343.1	0.64	Polyketide	aurachin A, aurachin D	Stigmatella aurantiaca Sg a15
BGC0001244.1	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 108672:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ohmal) + (ccmal - Me-ccmal - Me-ccmal - ccmal) + (Me-ohmal) + (ccmal - ccmal) + (ccmal)
Non-colinear ordering:: lobB + lobR + lobC + lobD + lobE + lobF

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

ORF7

Search NORINE for peptide: strict or relaxed

AMP-binding (47..440): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: tAiFFtmhgK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 40% (weak)

lobA

PKS_AT (569..846): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.1
inactive: 75.1
Methoxymalonyl-CoA: 52.7
Methylmalonyl-CoA: 48.6
Propionyl-CoA: 24.8
Isobutyryl-CoA: 17.9
Acetyl-CoA: 15.4
CHC-CoA: 15.0
Benzoyl-CoA: 14.4
Ethylmalonyl-CoA: 8.0
2-Methylbutyryl-CoA: 5.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

lobB

PKS_AT (541..818): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.7
inactive: 74.7
Methoxymalonyl-CoA: 52.4
Methylmalonyl-CoA: 46.8
Propionyl-CoA: 20.8
Isobutyryl-CoA: 18.9
Benzoyl-CoA: 12.2
Acetyl-CoA: 12.2
CHC-CoA: 10.4
Ethylmalonyl-CoA: 3.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (1983..2266): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.2
inactive: 75.6
Methoxymalonyl-CoA: 56.8
Methylmalonyl-CoA: 51.1
Propionyl-CoA: 25.4
Isobutyryl-CoA: 24.7
Benzoyl-CoA: 18.7
Acetyl-CoA: 18.5
CHC-CoA: 18.2
Ethylmalonyl-CoA: 12.0
2-Methylbutyryl-CoA: 11.0
fatty_acid: 9.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

lobC

PKS_AT (547..845): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.3
Ethylmalonyl-CoA: 196.6
Methoxymalonyl-CoA: 167.8
2-Methylbutyryl-CoA: 136.4
trans-1,2-CPDA: 126.6
Propionyl-CoA: 107.1
Malonyl-CoA: 91.8
CHC-CoA: 91.7
Isobutyryl-CoA: 89.5
Acetyl-CoA: 71.7
Benzoyl-CoA: 66.8
3-Methylbutyryl-CoA: 44.0
fatty_acid: 41.3
inactive: 35.8

lobD

PKS_AT (553..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 182.9
inactive: 74.2
Methoxymalonyl-CoA: 56.7
Methylmalonyl-CoA: 51.0
Isobutyryl-CoA: 25.3
Propionyl-CoA: 24.2
CHC-CoA: 19.1
Benzoyl-CoA: 18.7
Acetyl-CoA: 18.4
Ethylmalonyl-CoA: 11.0
2-Methylbutyryl-CoA: 10.4
fatty_acid: 9.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2253..2536): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.1
inactive: 75.1
Methoxymalonyl-CoA: 52.7
Methylmalonyl-CoA: 48.6
Propionyl-CoA: 24.8
Isobutyryl-CoA: 17.9
Acetyl-CoA: 15.4
CHC-CoA: 15.0
Benzoyl-CoA: 14.4
Ethylmalonyl-CoA: 8.0
2-Methylbutyryl-CoA: 5.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

lobE

PKS_AT (956..1232): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.0
inactive: 73.0
Methoxymalonyl-CoA: 51.2
Methylmalonyl-CoA: 43.7
Propionyl-CoA: 20.3
Isobutyryl-CoA: 13.7
Acetyl-CoA: 12.8
CHC-CoA: 11.8
Benzoyl-CoA: 11.3
Ethylmalonyl-CoA: 3.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

lobL

Search NORINE for peptide: strict or relaxed

AMP-binding (11..419): X

NRPSPredictor2: hydrophobic-aliphatic

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DflNLScIaK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 60% (weak)

lobM

Search NORINE for peptide: strict or relaxed

AMP-binding (17..421): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DcFFFaiVtK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 60% (weak)

lobN

PKS_AT (32..279): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 8.4
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

lobR

PKS_AT (668..966): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.5
inactive: 132.1
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 69.0
Isobutyryl-CoA: 46.3
Propionyl-CoA: 39.0
Ethylmalonyl-CoA: 34.1
2-Methylbutyryl-CoA: 24.3
Acetyl-CoA: 18.3
Benzoyl-CoA: 17.5
trans-1,2-CPDA: 9.0
fatty_acid: 7.4
CHC-CoA: 5.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2402..2700): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.1
Ethylmalonyl-CoA: 201.3
Methoxymalonyl-CoA: 171.3
2-Methylbutyryl-CoA: 155.5
trans-1,2-CPDA: 122.6
Propionyl-CoA: 112.0
Malonyl-CoA: 95.2
CHC-CoA: 88.3
Isobutyryl-CoA: 77.0
Benzoyl-CoA: 40.9
Acetyl-CoA: 40.8
3-Methylbutyryl-CoA: 38.2
fatty_acid: 27.5
inactive: 26.2

PKS_AT (4114..4412): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.4
Ethylmalonyl-CoA: 201.4
Methoxymalonyl-CoA: 169.2
2-Methylbutyryl-CoA: 157.2
trans-1,2-CPDA: 121.4
Propionyl-CoA: 109.3
Malonyl-CoA: 91.5
CHC-CoA: 86.0
Isobutyryl-CoA: 84.7
Benzoyl-CoA: 49.8
Acetyl-CoA: 40.3
3-Methylbutyryl-CoA: 37.9
fatty_acid: 27.4
inactive: 18.7

PKS_AT (5816..6114): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.5
inactive: 117.4
Methoxymalonyl-CoA: 63.8
Methylmalonyl-CoA: 53.5
Isobutyryl-CoA: 31.5
Propionyl-CoA: 23.1
Acetyl-CoA: 22.3
Ethylmalonyl-CoA: 21.2
Benzoyl-CoA: 18.8
trans-1,2-CPDA: 6.7
CHC-CoA: 5.9
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -0.5