BGC0001358 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001358 (Purpureocillium lilacinum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	119,575	High	leucinostatin A/leucinostatin B	NRPS:Type I+PKS

BGC0001358 - Region 1 - NRPS,T1PKS

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Location: 1 - 119,575 nt. (total: 119,575 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 119,576
Label		BGC0001358

Tailoring functions

Oxidoreductases: oxygenase

VFPBJ_02523: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

VFPBJ_02525: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

VFPBJ_02528: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: methyltransferase

VFPBJ_02521: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Ligases: ligase synthase

VFPBJ_02527: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

VFPBJ_02532: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

VFPBJ_02533: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

VFPBJ_02539: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001358.4	1.06	NRPS, PKS	leucinostatin A, leucinostatin B	Purpureocillium lilacinum
BGC0001825.4	0.57	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002191.2	0.45	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.40	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.39	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.39	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.38	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.34	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.34	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002238.3	0.34	PKS	2,4'-dihydroxy-3'-methoxypropiophenone	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001358.4	0.99	NRPS, PKS	leucinostatin A, leucinostatin B	Purpureocillium lilacinum
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 958 - 119575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 958 - 68967:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 43669 - 119575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - X - X - X - Leu - Leu - X - X - bAla - Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

VFPBJ_02527: pk

PKS_AT (581..887): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 54.2%; Propionyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 66.3
Methoxymalonyl-CoA: 45.3
Propionyl-CoA: 45.1
Isobutyryl-CoA: 36.5
Malonyl-CoA: 33.6
2-Methylbutyryl-CoA: 24.4
trans-1,2-CPDA: 22.2
fatty_acid: 20.6
Benzoyl-CoA: 10.4
inactive: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.2

VFPBJ_02532: pk

PKS_AT (446..708): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 62.5%; Methoxymalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 77.9
Methoxymalonyl-CoA: 65.7
Malonyl-CoA: 59.9
Ethylmalonyl-CoA: 50.7
fatty_acid: 32.6
Isobutyryl-CoA: 28.0
Propionyl-CoA: 26.4
2-Methylbutyryl-CoA: 25.9
CHC-CoA: 18.8
trans-1,2-CPDA: 18.7
Benzoyl-CoA: 18.3
inactive: 16.2
3-Methylbutyryl-CoA: 10.9
Acetyl-CoA: 9.1

VFPBJ_02533: X

AMP-binding (0..243): X

nrpys: meHex

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SLVWAGGGVK
Nearest Stachelhaus code(s):: meHex SLVWAGGGVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

VFPBJ_02539: X - X - X - X - Leu - Leu - X - X - bAla - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (708..1115): X

nrpys: 4Me-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYVMAAAK
Nearest Stachelhaus code(s):: 4Me-Pro DVFYVMAAAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1809..2212): X

nrpys: nohme-oxoDec

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAIGIGGVIK
Nearest Stachelhaus code(s):: nohme-oxoDec DAIGIGGVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2913..3316): X

nrpys: 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAILVGAIVK
Nearest Stachelhaus code(s):: 3OH-Leu DAILVGAIVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4043..4447): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: iva (Ival)

Stachelhaus prediction details:

Stachelhaus sequence:: DLGFLAGLFK
Nearest Stachelhaus code(s):: Aib DLGFLAGLFK (100% 8Å match); Abu or Ival DLGFLAGLFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5157..5571): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASLVGAVLK
Nearest Stachelhaus code(s):: Leu DASLVGAVLK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6253..6657): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALLVGAVAK
Nearest Stachelhaus code(s):: Leu DALLVGAVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7417..7828): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: iva (Ival)

Stachelhaus prediction details:

Stachelhaus sequence:: DLGFLAGVFK
Nearest Stachelhaus code(s):: Aib DLGFLAGVFK (100% 8Å match); Abu or Ival DLGFLAGVFK (97% 8Å match); Leu DLGFLAGVFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8558..8938): X

nrpys: Aib

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EISILIAFAK
Nearest Stachelhaus code(s):: Aib EISILIAFAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (9662..10068): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVAMVVAMFK
Nearest Stachelhaus code(s):: bAla DVAMVVAMFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (10799..11198): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVGMVGGIYK
Nearest Stachelhaus code(s):: Ala DVGMVGGIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor