BGC0001365 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001365 (Claviceps purpurea 20.1)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,indole	1	46,262	High	dithioclapurine/trithioclapurine/tetrathioclapurine	NRPS:Type I

BGC0001365 - Region 1 - NRPS,indole

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Location: 1 - 46,262 nt. (total: 46,262 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
indole: (indsynth or dmat or indole_PTase)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 46,263
Label		BGC0001365

Tailoring functions

Oxidoreductases: oxygenase reductase

CPUR_02677: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

CPUR_02681: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Transferases: aminotransferase sulfotransferase

CPUR_02676: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

CPUR_02679: aminotransferase

smcogs: SMCOG1019: aminotransferase

Ligases: ligase

CPUR_02680: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001365.3	1.93	NRPS	dithioclapurine, trithioclapurine, tetrathioclapurine	Claviceps purpurea 20.1
BGC0001135.5	0.57	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.57	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.54	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.54	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.54	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.53	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.53	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002321.3	0.33	other (other)	pulcherriminic acid, pulcherrimin, cyclodileucine, compound 3	Metschnikowia aff. pulcherrima
BGC0002518.3	0.32	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001365.3	0.99	NRPS	dithioclapurine, trithioclapurine, tetrathioclapurine	Claviceps purpurea 20.1
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.61	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002321.3	0.47	other (other)	pulcherriminic acid, pulcherrimin, cyclodileucine, compound 3	Metschnikowia aff. pulcherrima
BGC0002518.3	0.47	NRPS	syringafactin A, syringafactin C	Pseudomonas sp. SZ57

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 46262:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

CPUR_02680: Lys - X

Search NORINE for peptide: strict or relaxed

AMP-binding (12..395): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: lys (Lys)

Stachelhaus prediction details:

Stachelhaus sequence:: DAGPFGAVSK
Nearest Stachelhaus code(s):: Trp DVGPFGACTK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1070..1452): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVAMYIFIAK
Nearest Stachelhaus code(s):: Kyn DAAMIMGIAK (50% 8Å match); Asp DVWMYGAIVK (47% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor