Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001448.1:
Cluster 1NRP172298

BGC0001448, malacidin biosynthetic gene cluster from uncultured bacterium. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Malacidin A
ChEBI ID: 140173
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Ca+-dependent lipopeptide (cyclic)
methyl nonadienoic acid, methyl decadienoic acid
Thioesterase type:
Unknown
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
mlcA (ARU08063.1)
Module 2
A specificity: 2,3-Diamino-3-methylpropanoic acid
Evidence for specificity: Structure-based inference
C domain subtype: N/A
mlcK (ARU08073.1)
Module 1
A specificity: 3-methylaspartic acid
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 3
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Module 4
A specificity: Valine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
mlcL (ARU08074.1)
Module 5
A specificity: Lysine
Evidence for specificity: Structure-based inference
C domain subtype: Other
Module 6
A specificity: Hydroxyaspartic acid
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 7
A specificity: Aspartate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 8
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 9
A specificity: 3-methylaspartic acid
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
mlcM (ARU08075.1)
Module 10
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: DCL
Module 11
A specificity: 3-Methylproline
Evidence for specificity: Structure-based inference
C domain subtype: LCL

Gene cluster description

malacidin (BGC0001448). Gene Cluster 1. Biosynthetic class = NRP. GenBank KY654519. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Brad Hover (The Rockefeller University)

Literature references

1. Hover BM et al. (2018) Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens. Nat Microbiol 3(4):415-422. doi: 10.1038/s41564-018-0110-1. Epub 2018