Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001449.1:
Cluster 1NRP / Polyketide / Alkaloid142501

BGC0001449, UCS1025A biosynthetic gene cluster from Thermothelomyces thermophila. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: ucs1025a
PubChem ID: 11710200
SMILES string: Copy to clipboard
Molecular formula: C20H25NO5
Average molecular mass: 359.422 Da
Molecular activity: Antibacterial, Inhibitor
Molecular target: telomerase

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Alkaloid
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Reductive release

Nonribosomal peptide synthetases:
ucsA (AEO57481.1)
Module 2
A specificity: 3-Methylproline
Evidence for specificity: Feeding study
C domain subtype: Starter
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Iterative type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
MYCTH_68164
Iterative PKS subtype:
Highly reducing
6
Thioesterase type:
None
Release / cyclization type:
Claisen condensation

Modular polyketide synthases:
ucsA (AEO57481.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Inactive
Scaffold-modifying domain: Methylation
Module is iterated
Evidence for iteration: Unknown

Alkaloid subclass:
Pyrrolizidine

Gene cluster description

UCS1025A (BGC0001449). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Alkaloid. GenBank CP003004, positions 1181486-1217022. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Comments:Genetic and biochemical analysis revealed a a new strategy used by nature to construct heterocyclic alkaloid-like ring systems using assembly line logic.
Contact for this cluster:Li Li (College of Life Sciences, Fujian Normal University)

Literature references

1. Li L et al. (2018) Genome Mining and Assembly-Line Biosynthesis of the UCS1025A Pyrrolizidinone Family of Fungal Alkaloids. J Am Chem Soc 140(6):2067-2071. doi: 10.1021/jacs.8b00056. Epub 2018
2. Nakai R et al. (2006) Telomerase inhibitors identified by a forward chemical genetics approach using a yeast strain with shortened telomere length. Chem Biol 13(2):183-90. doi: 10.1016/j.chembiol.2005.11.010.
3. Agatsuma T et al. (2002) UCS1025A and B, new antitumor antibiotics from the fungus Acremonium species. Org Lett 4(25):4387-90.