BGC0001477 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001477 (Streptomyces sp. ID38640)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	116,700	High	lydicamycin	NRPS:Type I+PKS:Type I

BGC0001477 - Region 1 - NRPS,T1PKS

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Location: 1 - 116,700 nt. (total: 116,700 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 116,701
Label		BGC0001477

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AVV61981.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AVV61990.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase

AVV61977.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AVV61979.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AVV61982.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: amidase esterase

AVV61993.1: thioesterase

smcogs: SMCOG1004: thioesterase

AVV61996.1: amidase

smcogs: SMCOG1105: amidase

Ligases: ligase synthase

AVV61980.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AVV61983.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AVV61984.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AVV61985.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AVV61987.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AVV61989.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001477.5	1.35	NRPS, PKS	lydicamycin	Streptomyces sp. ID38640
BGC0001273.3	0.90	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.89	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001477.5	1.00	NRPS, PKS	lydicamycin	Streptomyces sp. ID38640
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 116700:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - Me-ccmal) + (ccmal - ccmal - Me-ccmal - ccmal) + (ccmal - Me-ccmal) + (Me-ccmal) + (redmal - Me-ccmal - ccmal) + (ohmal - redmal) + (Me-ccmal - ohmal) + (Gly) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AVV61977.1: pk

PKS_AT (61..299): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

AVV61979.1: mal - mmal

PKS_AT (571..868): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.4
inactive: 117.5
Methoxymalonyl-CoA: 51.7
Methylmalonyl-CoA: 46.3
Propionyl-CoA: 42.2
Isobutyryl-CoA: 21.4
Acetyl-CoA: 17.3
Ethylmalonyl-CoA: 15.1
CHC-CoA: 11.0
Benzoyl-CoA: 9.1
2-Methylbutyryl-CoA: 5.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2321..2616): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 176.5
trans-1,2-CPDA: 129.6
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 116.3
Propionyl-CoA: 112.0
Malonyl-CoA: 105.6
Isobutyryl-CoA: 76.0
Benzoyl-CoA: 63.2
Acetyl-CoA: 57.1
3-Methylbutyryl-CoA: 43.8
fatty_acid: 42.7
inactive: 40.5

AVV61980.1: mal - mal - mmal - mal

PKS_AT (561..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.6
inactive: 114.8
Methoxymalonyl-CoA: 54.4
Methylmalonyl-CoA: 50.3
Propionyl-CoA: 41.5
Acetyl-CoA: 18.3
Isobutyryl-CoA: 17.0
2-Methylbutyryl-CoA: 13.9
Ethylmalonyl-CoA: 11.3
fatty_acid: 9.4
Benzoyl-CoA: 7.8
CHC-CoA: 5.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2295..2588): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.7
inactive: 111.1
Methoxymalonyl-CoA: 54.8
Methylmalonyl-CoA: 49.2
Propionyl-CoA: 41.6
Acetyl-CoA: 19.9
Isobutyryl-CoA: 19.6
Ethylmalonyl-CoA: 12.2
2-Methylbutyryl-CoA: 9.8
CHC-CoA: 8.9
fatty_acid: 8.6
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4033..4330): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.9
Ethylmalonyl-CoA: 209.8
Methoxymalonyl-CoA: 175.9
trans-1,2-CPDA: 130.4
2-Methylbutyryl-CoA: 125.5
Propionyl-CoA: 107.5
CHC-CoA: 106.9
Malonyl-CoA: 103.0
Isobutyryl-CoA: 77.4
Benzoyl-CoA: 63.6
Acetyl-CoA: 58.3
inactive: 43.0
3-Methylbutyryl-CoA: 41.3
fatty_acid: 40.4

PKS_AT (5785..6086): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.8
inactive: 114.2
Methoxymalonyl-CoA: 56.5
Methylmalonyl-CoA: 55.3
Propionyl-CoA: 40.3
Isobutyryl-CoA: 22.7
Acetyl-CoA: 17.8
Ethylmalonyl-CoA: 16.9
fatty_acid: 12.4
2-Methylbutyryl-CoA: 10.3
CHC-CoA: 9.3
Benzoyl-CoA: 7.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61981.1: mal - mmal

PKS_AT (589..880): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.5
inactive: 115.5
Methoxymalonyl-CoA: 61.2
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 42.8
Isobutyryl-CoA: 25.8
Acetyl-CoA: 19.6
2-Methylbutyryl-CoA: 12.7
Ethylmalonyl-CoA: 10.9
fatty_acid: 9.4
Benzoyl-CoA: 8.4
CHC-CoA: 7.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2295..2590): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.9
Ethylmalonyl-CoA: 206.7
Methoxymalonyl-CoA: 176.5
trans-1,2-CPDA: 129.6
2-Methylbutyryl-CoA: 129.5
CHC-CoA: 116.3
Propionyl-CoA: 112.0
Malonyl-CoA: 105.6
Isobutyryl-CoA: 76.0
Benzoyl-CoA: 63.2
Acetyl-CoA: 57.1
3-Methylbutyryl-CoA: 43.8
fatty_acid: 42.7
inactive: 40.5

AVV61982.1: mmal

PKS_AT (576..871): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 179.7
2-Methylbutyryl-CoA: 137.4
trans-1,2-CPDA: 128.5
CHC-CoA: 117.6
Propionyl-CoA: 113.4
Malonyl-CoA: 106.1
Isobutyryl-CoA: 75.3
Benzoyl-CoA: 61.4
Acetyl-CoA: 52.3
inactive: 44.2
fatty_acid: 42.9
3-Methylbutyryl-CoA: 42.5

AVV61983.1: mal - mmal - mal

PKS_AT (579..869): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.2
inactive: 113.5
Methoxymalonyl-CoA: 55.2
Methylmalonyl-CoA: 48.3
Propionyl-CoA: 34.6
Acetyl-CoA: 24.2
Isobutyryl-CoA: 13.8
2-Methylbutyryl-CoA: 12.9
Benzoyl-CoA: 12.4
CHC-CoA: 12.0
fatty_acid: 11.6
Ethylmalonyl-CoA: 9.9
trans-1,2-CPDA: 5.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2674..2969): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.2
Ethylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 179.7
2-Methylbutyryl-CoA: 137.4
trans-1,2-CPDA: 128.5
CHC-CoA: 117.6
Propionyl-CoA: 113.4
Malonyl-CoA: 106.1
Isobutyryl-CoA: 75.3
Benzoyl-CoA: 61.4
Acetyl-CoA: 52.3
inactive: 44.2
fatty_acid: 42.9
3-Methylbutyryl-CoA: 42.5

PKS_AT (4427..4725): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.2
inactive: 113.6
Methoxymalonyl-CoA: 57.2
Methylmalonyl-CoA: 54.6
Propionyl-CoA: 40.7
Isobutyryl-CoA: 22.6
Acetyl-CoA: 18.0
Ethylmalonyl-CoA: 16.6
fatty_acid: 12.6
2-Methylbutyryl-CoA: 11.2
CHC-CoA: 9.1
Benzoyl-CoA: 7.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61984.1: mal - mal

PKS_AT (552..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.6
inactive: 112.0
Methoxymalonyl-CoA: 53.8
Methylmalonyl-CoA: 42.5
Propionyl-CoA: 40.6
Acetyl-CoA: 23.1
Isobutyryl-CoA: 20.3
2-Methylbutyryl-CoA: 13.3
Benzoyl-CoA: 8.3
Ethylmalonyl-CoA: 6.7
CHC-CoA: 6.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2053..2352): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.8
inactive: 104.2
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 47.7
Isobutyryl-CoA: 30.8
Propionyl-CoA: 30.7
Acetyl-CoA: 22.5
Ethylmalonyl-CoA: 12.0
Benzoyl-CoA: 10.6
CHC-CoA: 4.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.7

AVV61985.1: mmal - mal

PKS_AT (573..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.1
Ethylmalonyl-CoA: 202.5
Methoxymalonyl-CoA: 174.5
2-Methylbutyryl-CoA: 144.6
Propionyl-CoA: 127.1
trans-1,2-CPDA: 118.7
CHC-CoA: 105.8
Malonyl-CoA: 92.2
Isobutyryl-CoA: 78.8
Benzoyl-CoA: 72.2
fatty_acid: 42.8
Acetyl-CoA: 38.4
3-Methylbutyryl-CoA: 37.3
inactive: 24.3

PKS_AT (2328..2621): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.0
inactive: 102.0
Methoxymalonyl-CoA: 49.7
Methylmalonyl-CoA: 44.5
Propionyl-CoA: 35.0
Acetyl-CoA: 21.7
2-Methylbutyryl-CoA: 19.3
Isobutyryl-CoA: 15.9
Ethylmalonyl-CoA: 9.5
Benzoyl-CoA: 7.7
fatty_acid: 6.8
CHC-CoA: 2.9
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

AVV61987.1: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (458..843): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AVV61989.1: mal

PKS_AT (655..955): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.4
inactive: 117.1
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 49.8
Isobutyryl-CoA: 28.0
Ethylmalonyl-CoA: 27.5
Propionyl-CoA: 25.7
Acetyl-CoA: 23.7
Benzoyl-CoA: 14.0
2-Methylbutyryl-CoA: 10.4
CHC-CoA: 7.5
trans-1,2-CPDA: 7.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor