BGC0001492 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001492 (Streptomyces sp. LC-6-2)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	70,308	High	abyssomicin M/abyssomicin N/abyssomicin O/abyssomicin P/abyssomicin Q/abyssomicin R/abyssomicin S/abyssomicin T/abyssomicin U/abyssomicin V/abyssomicin W/abyssomicin X	PKS

BGC0001492 - Region 1 - T1PKS

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Location: 1 - 70,308 nt. (total: 70,308 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,309
Label		BGC0001492

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

ARE67839.1: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

ARE67841.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ARE67844.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

ARE67853.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ARE67858.1: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

ARE67864.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase acyltransferase

ARE67842.1: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

ARE67851.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ARE67852.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase phosphatase

ARE67850.1: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

ARE67865.1: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: cyclase

ARE67838.1: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: synthase

ARE67857.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001492.5	0.99	PKS	abyssomicin M, abyssomicin N, abyssomicin O, abyssomicin P, abyssomicin Q, abyssomicin R, abyssomicin S, abyssomicin T, abyssomicin U, abyssomicin V, abyssomicin W, abyssomicin X	Streptomyces sp. LC-6-2
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000037.4	0.66	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001492.5	1.00	PKS	abyssomicin M, abyssomicin N, abyssomicin O, abyssomicin P, abyssomicin Q, abyssomicin R, abyssomicin S, abyssomicin T, abyssomicin U, abyssomicin V, abyssomicin W, abyssomicin X	Streptomyces sp. LC-6-2
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000037.4	0.66	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69464:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - ccmal) + (Me-mal - Me-redmal) + (mal)
Non-colinear ordering:: ARE67853.1 + ARE67852.1 + ARE67851.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ARE67853.1: mal - mmal - mal - mal

PKS_AT (547..840): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.5
inactive: 94.0
Methoxymalonyl-CoA: 76.9
Methylmalonyl-CoA: 70.0
Isobutyryl-CoA: 42.5
Propionyl-CoA: 39.4
Ethylmalonyl-CoA: 38.8
2-Methylbutyryl-CoA: 25.2
Acetyl-CoA: 21.9
CHC-CoA: 19.7
fatty_acid: 16.1
Benzoyl-CoA: 15.9
trans-1,2-CPDA: 13.2
3-Methylbutyryl-CoA: 3.4

PKS_AT (1568..1859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.0
Ethylmalonyl-CoA: 208.9
Methoxymalonyl-CoA: 175.7
2-Methylbutyryl-CoA: 142.1
trans-1,2-CPDA: 139.3
Propionyl-CoA: 109.6
CHC-CoA: 97.0
Malonyl-CoA: 85.4
Isobutyryl-CoA: 84.1
Benzoyl-CoA: 68.8
Acetyl-CoA: 45.6
3-Methylbutyryl-CoA: 44.7
inactive: 24.4
fatty_acid: 23.2

PKS_AT (3377..3660): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.0
inactive: 75.6
Methoxymalonyl-CoA: 62.7
Methylmalonyl-CoA: 61.9
Isobutyryl-CoA: 32.1
Propionyl-CoA: 31.7
Ethylmalonyl-CoA: 31.5
trans-1,2-CPDA: 17.4
Acetyl-CoA: 17.2
Benzoyl-CoA: 16.0
2-Methylbutyryl-CoA: 9.8
fatty_acid: 8.5
3-Methylbutyryl-CoA: 6.1
CHC-CoA: 0.8

PKS_AT (5104..5396): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 164.2
Methoxymalonyl-CoA: 63.3
inactive: 62.5
Methylmalonyl-CoA: 58.9
Ethylmalonyl-CoA: 33.2
Propionyl-CoA: 22.7
Isobutyryl-CoA: 18.7
CHC-CoA: 15.0
Acetyl-CoA: 14.5
fatty_acid: 13.8
trans-1,2-CPDA: 11.8
3-Methylbutyryl-CoA: 11.8
Benzoyl-CoA: 11.7
2-Methylbutyryl-CoA: 10.7

ARE67852.1: mmal - mmal

PKS_AT (636..936): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.5
Ethylmalonyl-CoA: 189.7
Methoxymalonyl-CoA: 159.8
2-Methylbutyryl-CoA: 136.3
trans-1,2-CPDA: 126.9
Propionyl-CoA: 108.4
CHC-CoA: 99.9
Malonyl-CoA: 85.0
Isobutyryl-CoA: 75.1
Benzoyl-CoA: 57.2
3-Methylbutyryl-CoA: 47.3
Acetyl-CoA: 44.2
fatty_acid: 34.3
inactive: 13.7

PKS_AT (1965..2275): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 191.7
Ethylmalonyl-CoA: 171.8
Methoxymalonyl-CoA: 169.8
trans-1,2-CPDA: 117.1
2-Methylbutyryl-CoA: 113.5
Malonyl-CoA: 102.9
Propionyl-CoA: 95.8
CHC-CoA: 74.9
Isobutyryl-CoA: 67.5
Benzoyl-CoA: 47.4
3-Methylbutyryl-CoA: 41.5
Acetyl-CoA: 39.7
inactive: 25.9
fatty_acid: 18.2

ARE67851.1: mal

PKS_AT (570..874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 158.6
inactive: 69.8
Methoxymalonyl-CoA: 67.3
Methylmalonyl-CoA: 57.6
Propionyl-CoA: 56.8
Ethylmalonyl-CoA: 34.5
2-Methylbutyryl-CoA: 32.4
CHC-CoA: 29.7
trans-1,2-CPDA: 15.0
Acetyl-CoA: 13.5
fatty_acid: 11.0
Isobutyryl-CoA: 9.9
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor