BGC0001750 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001750 (Nannocystis pusilla)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	38,255	epothilone	NRP + Polyketide	50%

BGC0001750 - Region 1 - NRPS,T1PKS

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Location: 1 - 38,255 nt. (total: 38,255 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 38,256
Label		BGC0001750

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001750.1	1.59	NRP, Polyketide	pyrronazol B	Nannocystis pusilla
BGC0001273.1	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000041.1	0.62	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001275.1	0.62	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000343.1	0.60	NRP	enterobactin	Pseudomonas sp. J465
BGC0001276.1	0.59	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0000170.1	0.59	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0001068.1	0.59	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.58	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001244.1	0.57	Polyketide	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001750.1	0.93	NRP, Polyketide	pyrronazol B	Nannocystis pusilla
BGC0001273.1	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000041.1	0.64	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001275.1	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000343.1	0.64	NRP	enterobactin	Pseudomonas sp. J465
BGC0001276.1	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0000170.1	0.63	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0001068.1	0.63	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001858.1	0.63	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0001244.1	0.62	Polyketide	(-)-Mellein	Parastagonospora nodorum

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 38255:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X) + (Me-ccmal) + (Me-mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

pynC

Search NORINE for peptide: strict or relaxed

AMP-binding (9..400): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLlYLAlVcK
Nearest Stachelhaus code:: iva
Stachelhaus code match:: 70% (weak)

pynG

PKS_AT (587..889): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.7
Ethylmalonyl-CoA: 148.5
Methoxymalonyl-CoA: 134.6
Isobutyryl-CoA: 94.5
2-Methylbutyryl-CoA: 91.7
Malonyl-CoA: 91.3
trans-1,2-CPDA: 88.1
CHC-CoA: 82.6
Benzoyl-CoA: 80.3
Propionyl-CoA: 71.3
Acetyl-CoA: 59.4
fatty_acid: 37.4
3-Methylbutyryl-CoA: 34.7
inactive: 31.2

pynH

Search NORINE for peptide: strict or relaxed

AMP-binding (537..938): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLwNiasIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 60% (weak)

pynI

PKS_AT (600..888): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 164.9
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 134.1
Malonyl-CoA: 100.2
Benzoyl-CoA: 92.0
Isobutyryl-CoA: 84.4
trans-1,2-CPDA: 77.2
2-Methylbutyryl-CoA: 76.0
Propionyl-CoA: 74.4
Acetyl-CoA: 66.0
CHC-CoA: 52.3
3-Methylbutyryl-CoA: 38.9
fatty_acid: 30.9
inactive: 27.6

pynJ

PKS_AT (673..965): mmal

ATSignature: Methylmalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 70.8%; Malonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 127.1
Malonyl-CoA: 112.0
Methoxymalonyl-CoA: 109.2
Ethylmalonyl-CoA: 105.1
Isobutyryl-CoA: 78.6
trans-1,2-CPDA: 76.6
2-Methylbutyryl-CoA: 73.6
Propionyl-CoA: 68.1
fatty_acid: 52.5
CHC-CoA: 48.5
Benzoyl-CoA: 46.6
inactive: 40.1
Acetyl-CoA: 29.7
3-Methylbutyryl-CoA: 19.9