BGC0001856 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001856 (Streptomyces caniferus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like,PKS-like	1	186,923	High	caniferolide A/caniferolide B/caniferolide C/caniferolide D	PKS:Type I

BGC0001856 - Region 1 - NRPS-like,PKS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 186,923 nt. (total: 186,923 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 186,924
Label		BGC0001856

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

scaCP1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

scaCP2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

scaR3: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: PPTase acetyltransferase acylase aminotransferase glycosyltransferase sulfotransferase transferase

scaD: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

scaDH3: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

scaGS: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

scaGT1: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

scaGT2: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

scaK: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

scaP1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

scaPT: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

scaST: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Hydrolases: dehalogenase esterase

scaN: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

scaT: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

scaDH2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

scaR1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

scaR2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

scaG: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

scaL: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

scaP2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

scaP3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

scaP4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

scaP5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

scaP6: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

scaP7: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

scaS: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001856.4	1.53	PKS	caniferolide A, caniferolide B, caniferolide C, caniferolide D	Streptomyces caniferus
BGC0001276.3	1.34	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001249.5	1.13	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001273.3	1.10	PKS	asperlactone	Aspergillus ochraceus
BGC0001160.5	1.06	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	1.06	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.98	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.93	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.91	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.91	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001856.4	1.00	PKS	caniferolide A, caniferolide B, caniferolide C, caniferolide D	Streptomyces caniferus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0001164.3	0.70	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001249.5	0.70	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001160.5	0.70	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.65	NRPS, PKS	fusarin C	Fusarium fujikuroi

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 161352:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal) + (Me-ohmal - Me-ohmal - ccmal) + (ccmal - redmal) + (ohmal - redmal - ohmal) + (ohmal - ohmal - mal - Me-ohmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - ccmal) + (AHBA - pk) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 160286:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ccmal) + (Me-ohmal - Me-ohmal - ccmal) + (ccmal - redmal) + (ohmal - redmal - ohmal) + (ohmal - ohmal - mal - Me-ohmal - redmal) + (ohmal - redmal - ohmal) + (Me-ohmal - ccmal) + (AHBA - pk) + (Asn)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 120320 - 161352:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn) + (AHBA - pk) + (Me-ohmal - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

scaP1: mmal - mmal - mmal

PKS_AT (559..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 221.6
Ethylmalonyl-CoA: 219.6
Methoxymalonyl-CoA: 173.1
2-Methylbutyryl-CoA: 166.7
trans-1,2-CPDA: 136.4
CHC-CoA: 112.9
Propionyl-CoA: 112.0
Malonyl-CoA: 108.9
Isobutyryl-CoA: 63.0
Benzoyl-CoA: 55.4
Acetyl-CoA: 55.2
fatty_acid: 25.9
inactive: 23.3
3-Methylbutyryl-CoA: 19.6

PKS_AT (1570..1867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 225.9
Ethylmalonyl-CoA: 224.8
Methoxymalonyl-CoA: 183.1
2-Methylbutyryl-CoA: 153.8
trans-1,2-CPDA: 148.1
CHC-CoA: 110.9
Propionyl-CoA: 106.3
Malonyl-CoA: 101.2
Isobutyryl-CoA: 71.8
Benzoyl-CoA: 63.7
Acetyl-CoA: 52.1
3-Methylbutyryl-CoA: 29.8
fatty_acid: 27.7
inactive: 19.1

PKS_AT (3134..3431): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 224.5
Ethylmalonyl-CoA: 221.8
Methoxymalonyl-CoA: 182.2
2-Methylbutyryl-CoA: 166.5
trans-1,2-CPDA: 138.9
Propionyl-CoA: 112.3
CHC-CoA: 109.8
Malonyl-CoA: 108.1
Isobutyryl-CoA: 75.7
Benzoyl-CoA: 60.6
Acetyl-CoA: 57.4
fatty_acid: 34.0
3-Methylbutyryl-CoA: 30.9
inactive: 28.5

scaP2: mmal - mmal - mal

PKS_AT (575..872): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 227.3
Ethylmalonyl-CoA: 222.5
Methoxymalonyl-CoA: 186.3
2-Methylbutyryl-CoA: 159.9
trans-1,2-CPDA: 144.1
CHC-CoA: 117.7
Propionyl-CoA: 109.6
Malonyl-CoA: 109.3
Isobutyryl-CoA: 83.7
Benzoyl-CoA: 70.5
Acetyl-CoA: 52.7
3-Methylbutyryl-CoA: 42.3
fatty_acid: 30.1
inactive: 21.0

PKS_AT (2123..2420): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 227.9
Ethylmalonyl-CoA: 223.5
Methoxymalonyl-CoA: 186.6
2-Methylbutyryl-CoA: 163.4
trans-1,2-CPDA: 143.4
CHC-CoA: 125.6
Propionyl-CoA: 115.0
Malonyl-CoA: 111.5
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 65.8
Acetyl-CoA: 53.4
3-Methylbutyryl-CoA: 49.1
fatty_acid: 33.0
inactive: 26.9

PKS_AT (3665..3959): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.2
inactive: 122.5
Methoxymalonyl-CoA: 67.0
Methylmalonyl-CoA: 56.2
Propionyl-CoA: 36.8
2-Methylbutyryl-CoA: 25.7
Ethylmalonyl-CoA: 23.2
Isobutyryl-CoA: 21.5
Acetyl-CoA: 20.2
Benzoyl-CoA: 18.1
fatty_acid: 14.5
CHC-CoA: 8.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

scaP3: mal - mal

PKS_AT (576..872): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.1
inactive: 108.3
Methoxymalonyl-CoA: 59.8
Methylmalonyl-CoA: 56.2
Propionyl-CoA: 39.8
2-Methylbutyryl-CoA: 35.9
Isobutyryl-CoA: 29.3
Benzoyl-CoA: 25.5
Ethylmalonyl-CoA: 21.6
Acetyl-CoA: 19.2
CHC-CoA: 15.9
fatty_acid: 15.7
trans-1,2-CPDA: 14.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2333..2625): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.7
inactive: 111.7
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 37.5
Propionyl-CoA: 32.4
Acetyl-CoA: 19.5
Isobutyryl-CoA: 17.6
2-Methylbutyryl-CoA: 11.0
Ethylmalonyl-CoA: 10.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

scaP4: mal - mal - mal

PKS_AT (595..888): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.2
inactive: 118.5
Methoxymalonyl-CoA: 71.3
Methylmalonyl-CoA: 61.4
Propionyl-CoA: 32.1
Isobutyryl-CoA: 30.8
Acetyl-CoA: 24.0
Benzoyl-CoA: 20.6
fatty_acid: 19.1
Ethylmalonyl-CoA: 15.5
CHC-CoA: 15.4
2-Methylbutyryl-CoA: 10.6
trans-1,2-CPDA: 7.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2147..2444): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.8
inactive: 131.0
Methoxymalonyl-CoA: 62.9
Methylmalonyl-CoA: 54.3
Isobutyryl-CoA: 34.7
Benzoyl-CoA: 30.0
Propionyl-CoA: 27.7
Ethylmalonyl-CoA: 22.5
Acetyl-CoA: 22.0
fatty_acid: 14.8
trans-1,2-CPDA: 9.9
2-Methylbutyryl-CoA: 7.7
CHC-CoA: 6.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (4229..4522): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.9
inactive: 114.5
Methoxymalonyl-CoA: 63.9
Methylmalonyl-CoA: 58.1
Propionyl-CoA: 36.3
Isobutyryl-CoA: 24.8
Ethylmalonyl-CoA: 24.0
Benzoyl-CoA: 23.1
Acetyl-CoA: 22.2
2-Methylbutyryl-CoA: 20.3
fatty_acid: 15.9
CHC-CoA: 14.7
trans-1,2-CPDA: 12.6
3-Methylbutyryl-CoA: 0.0

scaP5: mal - mal - mal - mmal - mal

PKS_AT (572..866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.5
inactive: 137.5
Methoxymalonyl-CoA: 65.7
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 32.4
Isobutyryl-CoA: 32.4
Acetyl-CoA: 26.3
Benzoyl-CoA: 25.9
Ethylmalonyl-CoA: 19.0
CHC-CoA: 15.5
fatty_acid: 13.5
2-Methylbutyryl-CoA: 11.9
trans-1,2-CPDA: 9.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2099..2392): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.2
inactive: 116.0
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 53.3
Propionyl-CoA: 32.5
Isobutyryl-CoA: 26.9
Ethylmalonyl-CoA: 23.5
Benzoyl-CoA: 21.0
Acetyl-CoA: 18.4
CHC-CoA: 18.1
fatty_acid: 14.5
2-Methylbutyryl-CoA: 11.4
trans-1,2-CPDA: 8.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (3556..3852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.8
inactive: 128.1
Methoxymalonyl-CoA: 61.7
Methylmalonyl-CoA: 57.1
Propionyl-CoA: 32.6
Isobutyryl-CoA: 29.4
Acetyl-CoA: 22.3
Benzoyl-CoA: 21.0
Ethylmalonyl-CoA: 18.2
CHC-CoA: 15.8
fatty_acid: 13.1
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (5259..5556): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.1
Ethylmalonyl-CoA: 207.2
Methoxymalonyl-CoA: 168.5
2-Methylbutyryl-CoA: 161.5
trans-1,2-CPDA: 135.8
Propionyl-CoA: 111.8
CHC-CoA: 105.1
Malonyl-CoA: 83.9
Isobutyryl-CoA: 79.4
Benzoyl-CoA: 54.5
Acetyl-CoA: 43.9
3-Methylbutyryl-CoA: 41.2
fatty_acid: 23.4
inactive: 22.5

PKS_AT (6820..7121): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.7
inactive: 124.5
Methoxymalonyl-CoA: 67.0
Methylmalonyl-CoA: 62.0
Propionyl-CoA: 39.2
Isobutyryl-CoA: 34.1
Ethylmalonyl-CoA: 29.2
2-Methylbutyryl-CoA: 26.6
Benzoyl-CoA: 25.2
Acetyl-CoA: 21.6
CHC-CoA: 16.0
fatty_acid: 15.3
trans-1,2-CPDA: 12.7
3-Methylbutyryl-CoA: 0.0

scaP6: mal - mal - mal

PKS_AT (573..874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.0
inactive: 117.9
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 50.6
Propionyl-CoA: 30.5
Isobutyryl-CoA: 29.5
Benzoyl-CoA: 21.6
Acetyl-CoA: 21.2
Ethylmalonyl-CoA: 17.6
fatty_acid: 11.9
2-Methylbutyryl-CoA: 11.0
trans-1,2-CPDA: 9.5
CHC-CoA: 5.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (2045..2341): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.8
inactive: 128.1
Methoxymalonyl-CoA: 61.7
Methylmalonyl-CoA: 57.1
Propionyl-CoA: 32.6
Isobutyryl-CoA: 29.4
Acetyl-CoA: 22.3
Benzoyl-CoA: 21.0
Ethylmalonyl-CoA: 18.2
CHC-CoA: 15.8
fatty_acid: 13.1
2-Methylbutyryl-CoA: 11.2
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (4163..4463): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.5
inactive: 102.0
Methoxymalonyl-CoA: 66.3
Methylmalonyl-CoA: 40.6
Propionyl-CoA: 33.9
Isobutyryl-CoA: 26.9
Ethylmalonyl-CoA: 24.2
Benzoyl-CoA: 17.8
Acetyl-CoA: 17.7
2-Methylbutyryl-CoA: 16.4
fatty_acid: 12.5
CHC-CoA: 2.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

scaP7: mmal - mal

PKS_AT (570..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 219.8
Ethylmalonyl-CoA: 213.9
Methoxymalonyl-CoA: 178.6
2-Methylbutyryl-CoA: 163.9
trans-1,2-CPDA: 136.8
Propionyl-CoA: 122.2
Malonyl-CoA: 107.4
CHC-CoA: 104.4
Isobutyryl-CoA: 87.1
Benzoyl-CoA: 72.1
Acetyl-CoA: 54.3
3-Methylbutyryl-CoA: 44.1
fatty_acid: 33.2
inactive: 30.6

PKS_AT (2116..2409): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.2
inactive: 127.3
Methoxymalonyl-CoA: 66.3
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 35.9
Isobutyryl-CoA: 27.3
Acetyl-CoA: 22.8
Ethylmalonyl-CoA: 19.1
Benzoyl-CoA: 18.6
CHC-CoA: 15.9
2-Methylbutyryl-CoA: 13.6
fatty_acid: 13.4
trans-1,2-CPDA: 10.8
3-Methylbutyryl-CoA: 0.0

scaP8: AHBA - pk

CAL_domain (33..441): AHBA

minowa: AHBA

Prediction, score:

AHBA: 324.7
shikimic_acid: 245.8
Acetyl-CoA: 12.0
fatty_acid: 0.0
NH2: 0.0

PKS_AT (1184..1480): pk

ATSignature: Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 205.9
Methylmalonyl-CoA: 198.4
Methoxymalonyl-CoA: 167.2
2-Methylbutyryl-CoA: 139.5
trans-1,2-CPDA: 125.1
CHC-CoA: 109.0
Propionyl-CoA: 106.9
Malonyl-CoA: 90.5
Benzoyl-CoA: 52.0
Isobutyryl-CoA: 48.4
3-Methylbutyryl-CoA: 42.4
Acetyl-CoA: 37.2
fatty_acid: 30.8
inactive: 16.5

scaG: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (415..815): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVTNIIEIGK
Nearest Stachelhaus code(s):: Asn DGTKIGEIGK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

scaH5: pk

PKS_AT (31..280): pk

ATSignature: (unknown)

No matches above 50%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 14.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

scaJ: X

AMP-binding (97..309): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---EGGAFGK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

scaL: X

AMP-binding (240..427): X

nrpys: (unknown)

SVM prediction details:

NOTE: uncertain match

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---FVIELPK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor