BGC0001963 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001963 (Catenulispora sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS-like,NRPS	1	154,666	High	catenulisporolide A/catenulisporolide B/catenulisporolide C/catenulisporolide D	PKS:Type I

BGC0001963 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 154,666 nt. (total: 154,666 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 154,667
Label		BGC0001963

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

AZF85917.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

AZF85924.1: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

AZF85937.1: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

Transferases: PPTase acylase aminotransferase glycosyltransferase

AZF85904.1: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

AZF85915.1: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

AZF85919.1: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

AZF85926.1: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

AZF85940.1: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

AZF85947.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

AZF85923.1: thioesterase

smcogs: SMCOG1004: thioesterase

AZF85939.1: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

AZF85899.1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

AZF85912.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85929.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85930.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85933.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85934.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AZF85941.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AZF85942.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85944.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AZF85945.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AZF85946.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001963.2	2.27	PKS	catenulisporolide A, catenulisporolide B, catenulisporolide C, catenulisporolide D	Catenulispora sp.
BGC0001164.3	1.97	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	1.96	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001132.4	1.92	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001275.3	1.90	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002286.3	1.86	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	1.85	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	1.83	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	1.79	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	1.79	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001963.2	0.99	PKS	catenulisporolide A, catenulisporolide B, catenulisporolide C, catenulisporolide D	Catenulispora sp.
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0002075.3	0.76	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 154666:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal - pk - ccmal) + (ccmal - ccmal - ohmal - ohmal) + (ohmal - pk - mal - ohmal - redmal) + (redmal) + (Asp) + (X - Asn) + (X) + (pk - Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 31935:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 13731 - 56824:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 29287 - 154666:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal - pk - ccmal) + (ccmal - ccmal - ohmal - ohmal) + (ohmal - pk - mal - ohmal - redmal) + (redmal) + (Asp) + (X - Asn) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 124814 - 154666:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ohmal - pk - ccmal) + (ccmal - ccmal - ohmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AZF85917.1: pk - mmal

PKS_AT (677..973): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 206.5
Methylmalonyl-CoA: 202.9
Methoxymalonyl-CoA: 160.6
2-Methylbutyryl-CoA: 137.1
trans-1,2-CPDA: 121.7
Propionyl-CoA: 104.0
Malonyl-CoA: 90.6
CHC-CoA: 80.4
Benzoyl-CoA: 80.4
Isobutyryl-CoA: 72.9
fatty_acid: 35.2
Acetyl-CoA: 33.7
3-Methylbutyryl-CoA: 30.3
inactive: 26.5

PKS_AT (2259..2556): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 208.3
Ethylmalonyl-CoA: 195.0
Methoxymalonyl-CoA: 163.5
2-Methylbutyryl-CoA: 133.6
Propionyl-CoA: 102.3
Malonyl-CoA: 96.9
trans-1,2-CPDA: 94.8
CHC-CoA: 85.1
Isobutyryl-CoA: 79.1
Benzoyl-CoA: 56.1
Acetyl-CoA: 45.6
inactive: 34.4
fatty_acid: 27.2
3-Methylbutyryl-CoA: 25.6

AZF85929.1: X

AMP-binding (415..807): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLTSVVMVGK
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

AZF85930.1: X

AMP-binding (18..483): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMTSLGCCYK
Nearest Stachelhaus code(s):: Pca DMGVLGCLMK (65% 8Å match); omeTyr DMGSHGCFCK (44% 8Å match); Ile DAMFLGCTYK (26% 8Å match)
Stachelhaus code match:: 60% (weak)

AZF85932.1: mal

PKS_AT (636..933): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 150.3
inactive: 107.6
Methoxymalonyl-CoA: 65.5
Methylmalonyl-CoA: 61.6
Isobutyryl-CoA: 44.5
Propionyl-CoA: 40.2
Benzoyl-CoA: 22.8
Ethylmalonyl-CoA: 22.5
CHC-CoA: 20.0
2-Methylbutyryl-CoA: 15.4
3-Methylbutyryl-CoA: 13.4
fatty_acid: 12.8
trans-1,2-CPDA: 9.9
Acetyl-CoA: 8.9

AZF85933.1: X

AMP-binding (16..415): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GFGCAGLNTK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AZF85940.1: X - X - Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (689..1098): X

nrpys: Glu, Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPHQLGVIEK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1748..2131): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DADEAGTVTK
Nearest Stachelhaus code(s):: Lys DADDAGTVDK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2798..3201): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DFPKLGEVAK
Nearest Stachelhaus code(s):: Asn DGTKLGEVGK (68% 8Å match)
Stachelhaus code match:: 70% (weak)

AZF85912.1: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (428..819): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGHVGK
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AZF85941.1: mal - mal

PKS_AT (220..510): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.0
inactive: 108.6
Methoxymalonyl-CoA: 51.9
Methylmalonyl-CoA: 43.1
Propionyl-CoA: 26.2
Benzoyl-CoA: 18.4
Acetyl-CoA: 15.7
fatty_acid: 14.9
Isobutyryl-CoA: 14.9
Ethylmalonyl-CoA: 13.4
trans-1,2-CPDA: 9.1
CHC-CoA: 5.5
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (2314..2612): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.7
inactive: 112.9
Methoxymalonyl-CoA: 58.3
Methylmalonyl-CoA: 49.4
Propionyl-CoA: 37.4
Isobutyryl-CoA: 20.8
Benzoyl-CoA: 19.2
Acetyl-CoA: 18.7
fatty_acid: 17.0
Ethylmalonyl-CoA: 16.8
2-Methylbutyryl-CoA: 12.2
CHC-CoA: 11.6
trans-1,2-CPDA: 10.1
3-Methylbutyryl-CoA: 0.0

AZF85947.1: mal - pk - mal - mal

PKS_AT (583..873): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.1
inactive: 107.7
Methylmalonyl-CoA: 51.4
Methoxymalonyl-CoA: 51.1
Propionyl-CoA: 37.2
Benzoyl-CoA: 19.6
Isobutyryl-CoA: 18.5
Acetyl-CoA: 17.6
fatty_acid: 15.2
CHC-CoA: 11.4
Ethylmalonyl-CoA: 10.0
2-Methylbutyryl-CoA: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2040..2340): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 203.9
Methylmalonyl-CoA: 202.1
Methoxymalonyl-CoA: 171.1
2-Methylbutyryl-CoA: 136.4
trans-1,2-CPDA: 123.3
Propionyl-CoA: 109.0
CHC-CoA: 96.9
Malonyl-CoA: 93.1
Isobutyryl-CoA: 73.6
Benzoyl-CoA: 61.5
3-Methylbutyryl-CoA: 42.9
inactive: 36.2
fatty_acid: 30.5
Acetyl-CoA: 22.9

PKS_AT (3661..3943): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.8
inactive: 120.8
Methoxymalonyl-CoA: 59.0
Methylmalonyl-CoA: 54.0
Propionyl-CoA: 43.0
Benzoyl-CoA: 26.3
Isobutyryl-CoA: 23.9
Ethylmalonyl-CoA: 22.9
Acetyl-CoA: 22.7
2-Methylbutyryl-CoA: 15.9
CHC-CoA: 15.8
fatty_acid: 11.4
trans-1,2-CPDA: 8.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (4992..5280): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 230.1
inactive: 121.7
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 34.4
Isobutyryl-CoA: 25.3
Ethylmalonyl-CoA: 22.4
CHC-CoA: 22.4
Benzoyl-CoA: 22.3
Acetyl-CoA: 20.7
2-Methylbutyryl-CoA: 14.8
fatty_acid: 14.2
trans-1,2-CPDA: 11.5
3-Methylbutyryl-CoA: 0.0

AZF85946.1: mal - mal - mal - mal

PKS_AT (579..871): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.8
inactive: 111.0
Methoxymalonyl-CoA: 57.5
Methylmalonyl-CoA: 56.1
Propionyl-CoA: 44.8
Ethylmalonyl-CoA: 25.7
Isobutyryl-CoA: 24.4
Acetyl-CoA: 23.5
Benzoyl-CoA: 22.3
CHC-CoA: 18.6
fatty_acid: 17.5
2-Methylbutyryl-CoA: 12.9
trans-1,2-CPDA: 10.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2274..2563): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.0
inactive: 113.1
Methoxymalonyl-CoA: 57.3
Methylmalonyl-CoA: 56.3
Propionyl-CoA: 38.3
Isobutyryl-CoA: 29.5
Benzoyl-CoA: 24.5
Acetyl-CoA: 20.3
Ethylmalonyl-CoA: 19.9
fatty_acid: 19.6
2-Methylbutyryl-CoA: 15.5
CHC-CoA: 15.0
trans-1,2-CPDA: 12.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (3998..4287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 109.3
Methoxymalonyl-CoA: 51.8
Methylmalonyl-CoA: 47.9
Propionyl-CoA: 45.1
Isobutyryl-CoA: 27.5
Benzoyl-CoA: 23.5
fatty_acid: 19.1
2-Methylbutyryl-CoA: 17.5
Acetyl-CoA: 17.0
CHC-CoA: 13.0
Ethylmalonyl-CoA: 10.8
trans-1,2-CPDA: 9.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (5476..5766): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.9
inactive: 112.2
Methoxymalonyl-CoA: 64.0
Methylmalonyl-CoA: 58.6
Propionyl-CoA: 35.2
Isobutyryl-CoA: 22.9
Acetyl-CoA: 22.9
Ethylmalonyl-CoA: 21.5
Benzoyl-CoA: 19.9
2-Methylbutyryl-CoA: 16.4
CHC-CoA: 12.0
fatty_acid: 11.8
trans-1,2-CPDA: 9.8
3-Methylbutyryl-CoA: 0.0

AZF85945.1: mal - pk - mal

PKS_AT (628..921): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.7
inactive: 103.9
Methoxymalonyl-CoA: 68.4
Methylmalonyl-CoA: 65.9
Propionyl-CoA: 38.8
CHC-CoA: 25.3
fatty_acid: 25.2
Isobutyryl-CoA: 23.4
Acetyl-CoA: 23.1
2-Methylbutyryl-CoA: 22.1
Ethylmalonyl-CoA: 18.8
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 14.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2131..2428): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 205.6
Methylmalonyl-CoA: 205.2
Methoxymalonyl-CoA: 160.3
2-Methylbutyryl-CoA: 127.0
trans-1,2-CPDA: 124.5
Propionyl-CoA: 95.3
Malonyl-CoA: 89.5
Isobutyryl-CoA: 73.9
CHC-CoA: 71.6
Benzoyl-CoA: 66.9
Acetyl-CoA: 49.4
3-Methylbutyryl-CoA: 26.8
fatty_acid: 26.6
inactive: 25.7

PKS_AT (3678..3968): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.8
inactive: 106.6
Methoxymalonyl-CoA: 53.0
Methylmalonyl-CoA: 51.2
Propionyl-CoA: 39.0
Isobutyryl-CoA: 20.8
Acetyl-CoA: 18.8
Benzoyl-CoA: 16.4
fatty_acid: 12.7
CHC-CoA: 11.8
Ethylmalonyl-CoA: 10.7
2-Methylbutyryl-CoA: 9.5
trans-1,2-CPDA: 9.1
3-Methylbutyryl-CoA: 0.0

AZF85944.1: X

AMP-binding (448..840): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMTSLASIEK
Nearest Stachelhaus code(s):: Pro DMTFSAGIIK (59% 8Å match)
Stachelhaus code match:: 60% (weak)

AZF85942.1: NH2

CAL_domain (45..484): NH2

minowa: NH2

Prediction, score:

NH2: 114.9
fatty_acid: 19.5
AHBA: 1.5
shikimic_acid: 0.0
Acetyl-CoA: -3.1

AZF85914.1: pk

PKS_AT (23..264): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor