BGC0002097 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002097 (Micromonospora ferruginea)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,ectoine	1	72,474	High	kosinostatin	NRPS:Type I+PKS:Type II+saccharide:hybrid/tailoring

BGC0002097 - Region 1 - T2PKS,ectoine

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Location: 1 - 72,474 nt. (total: 72,474 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
ectoine: ectoine_synt

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,475
Label		BGC0002097

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

H1D33_25560: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

H1D33_25590: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

H1D33_25595: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

H1D33_25710: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

H1D33_25720: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

H1D33_25750: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: glycosyltransferase methyltransferase transferase

H1D33_25555: methyltransferase

smcogs: SMCOG1089: methyltransferase

H1D33_25625: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

H1D33_25730: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: hydrolase

H1D33_25565: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase dehydratase

H1D33_25715: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

H1D33_25725: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

H1D33_25610: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

H1D33_25695: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

H1D33_25700: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H1D33_25775: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002097.3	1.23	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001073.5	0.71	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0001250.4	0.52	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.52	NRPS	epichloenin A	Epichloe festucae
BGC0001166.5	0.51	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.51	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001268.4	0.49	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000231.5	0.45	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000852.5	0.36	other (other)	ectoine	Sporosarcina pasteurii
BGC0001778.5	0.36	other (other)	showdomycin	Streptomyces showdoensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002097.3	0.99	NRPS, PKS, saccharide	kosinostatin	Micromonospora sp. 28ISP2-46
BGC0001073.5	0.81	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0000231.5	0.76	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.67	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0002276.2	0.65	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001723.5	0.64	PKS	oviedomycin	Streptomyces antibioticus
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus
BGC0001268.4	0.61	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0000218.5	0.60	PKS	doxorubicin	Streptomyces peucetius
BGC0001250.4	0.59	NRPS	epichloenin A	Epichloe festucae

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

H1D33_25775: X

AMP-binding (12..390): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLQITLDSK
Nearest Stachelhaus code(s):: Ala DILQITLVYK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 72474
Starter unit(s):: acetyl-CoA (Score: 0.0; E-value: 0)
Malonyl elongations:: 8|9 (Score: 640.7; E-value: 1.9e-195)
Product class(es):: anthracycline
Product molecular weight(s):: acetyl-CoA_8: 498 Da
acetyl-CoA_9: 540 Da

H1D33_25555: MET carboxy_O (Score: 368.8; E-value: 2.8e-113)
H1D33_25625: GT (Score: 453.0; E-value: 2.8e-138)
H1D33_25640: CYC C1-C18|C2-C19 (Score: 189.4; E-value: 6.1e-59)
H1D33_25675: DIMER (Score: 272.5; E-value: 9.2e-84)
H1D33_25685: CYC C2-C19 (Score: 288.8; E-value: 1.4e-89)
H1D33_25690: ACP (Score: 115.6; E-value: 1.7e-36)
H1D33_25695: CLF 8|9 (Score: 640.7; E-value: 1.9e-195)
H1D33_25700: KS (Score: 764.6; E-value: 5.6e-233)
H1D33_25710: KR C9 (Score: 455.3; E-value: 1.3e-139)
H1D33_25715: CYC C7-C12 (Score: 472.5; E-value: 1.1e-144)
H1D33_25720: KR C17 (Score: 391.3; E-value: 3.9e-120)
H1D33_25780: CYC C5-C14/C3-C16 (Score: 433.5; E-value: 6.2e-133)