BGC0002137 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002137 (Streptomyces sp. NRRL F-3213)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,PKS-like	1	58,449	High	mutaxanthene A/mutaxanthene B/mutaxanthene D	PKS

BGC0002137 - Region 1 - PKS-like,T2PKS

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Location: 1 - 58,449 nt. (total: 58,449 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 58,450
Label		BGC0002137

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

IF70_RS33705: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IF70_RS33720: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IF70_RS33725: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

IF70_RS33740: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IF70_RS33775: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

IF70_RS33780: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

IF70_RS33790: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase methyltransferase

IF70_RS33690: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

IF70_RS33745: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

IF70_RS33785: Methylation

MITE: MITE0000023Methylation (identity: 60.80%)
smcogs: SMCOG1042: O-methyltransferase

Lyases: cyclase

IF70_RS33715: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: ligase synthase

IF70_RS33695: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

IF70_RS33710: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

IF70_RS33760: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

IF70_RS33765: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002137.3	1.78	PKS	mutaxanthene A, mutaxanthene B, mutaxanthene D	Streptomyces sp. NRRL F-3213
BGC0000231.5	0.94	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.68	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.58	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.57	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000272.3	0.55	PKS	spore pigment	Streptomyces collinus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002137.3	0.98	PKS	mutaxanthene A, mutaxanthene B, mutaxanthene D	Streptomyces sp. NRRL F-3213
BGC0000231.5	0.77	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.68	PKS	jadomycin	Streptomyces venezuelae ATCC 10712
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.62	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000272.3	0.61	PKS	spore pigment	Streptomyces collinus

Similar known clusters from MIBiG 4.0

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

Type II PKS

NRPS/PKS substrate predictions

IF70_RS33690: mal

PKS_AT (6..296): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 58.4
Methoxymalonyl-CoA: 43.2
Methylmalonyl-CoA: 40.6
Propionyl-CoA: 22.5
3-Methylbutyryl-CoA: 16.0
inactive: 12.8
2-Methylbutyryl-CoA: 10.5
Isobutyryl-CoA: 9.2
Ethylmalonyl-CoA: 8.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0

IF70_RS33710: X

AMP-binding (24..408): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PMNYLGGPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 58449
Starter unit(s):: propionyl-CoA (Score: 702.0; E-value: 2.21e-139)
Malonyl elongations:: 8|9 (Score: 631.9; E-value: 8.4e-193)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: propionyl-CoA_8: 415 Da
propionyl-CoA_9: 457 Da

IF70_RS33690: AT (Score: 406.2; E-value: 2.4e-124); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 312.0; E-value: 1.24e-108)
IF70_RS33695: KSIII (Score: 427.3; E-value: 9.3e-131); KSIII ABC00733.1_Cosmomycin_propionyl-CoA (Score: 390.0; E-value: 2.21e-139)
IF70_RS33700: CYC C5-C14/C3-C16 (Score: 400.9; E-value: 5.2e-123)
IF70_RS33705: KR (Score: 148.7; E-value: 5.7e-46)
IF70_RS33715: CYC C7-C12 (Score: 438.5; E-value: 2.5e-134)
IF70_RS33720: KR C9 (Score: 469.3; E-value: 6.9e-144)
IF70_RS33725: OXY (Score: 172.9; E-value: 2.9e-53)
IF70_RS33735: CYC C1-C18|C2-C19 (Score: 191.5; E-value: 1.3e-59)
IF70_RS33740: KR (Score: 143.4; E-value: 2.3e-44)
IF70_RS33745: MET (Score: 259.8; E-value: 8.4e-80)
IF70_RS33755: ACP (Score: 110.0; E-value: 1e-34)
IF70_RS33760: CLF 8|9 (Score: 631.9; E-value: 8.4e-193)
IF70_RS33765: KS (Score: 745.8; E-value: 2.7e-227)
IF70_RS33775: KR (Score: 181.8; E-value: 4.6e-56)
IF70_RS33780: OXY (Score: 402.1; E-value: 8.7e-123)
IF70_RS33785: MET C2O|C2N (Score: 403.2; E-value: 1.9e-123)
IF70_RS33790: OXY (Score: 511.9; E-value: 4.3e-156)