BGC0002441 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002441 (Streptomyces sp. SANK 60404)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	arylpolyene,NRPS-like	1	28,832	High	(2E,4E,6E,8E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-9-(o-tolyl)nona-2,4,6,8-tetraenamide/(E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-5-(o-tolyl)pent-4-enamide	PKS+NRPS:Type I

BGC0002441 - Region 1 - NRPS-like,arylpolyene

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Location: 1 - 28,832 nt. (total: 28,832 nt) Show pHMM detection rules used

Region on contig edge.

arylpolyene: (APE_KS1 or APE_KS2)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 28,833
Label		BGC0002441

Tailoring functions

Oxidoreductases: dehydrogenase

mbg25: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: PPTase acylase

mbg0: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

mbg1: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Lyases: dehydratase

mbg11: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

orf2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

mbg16: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mbg21: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mbg3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mbg5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mbg6: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mbg7: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

orf3: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002441.2	1.90	NRPS, PKS	(2E,4E,6E,8E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-9-(o-tolyl)nona-2,4,6,8-tetraenamide, (E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-5-(o-tolyl)pent-4-enamide	Streptomyces sp. SANK 60404
BGC0002274.2	0.63	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002277.2	0.63	NRPS	atromentin	Suillus grevillei
BGC0002193.2	0.61	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0001276.3	0.56	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001833.4	0.54	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002174.2	0.51	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000340.5	0.38	NRPS	echoside A, echoside B, echoside C, echoside D, echoside E	Streptomyces sp. LZ35
BGC0001075.5	0.34	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0001987.2	0.34	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002441.2	1.00	NRPS, PKS	(2E,4E,6E,8E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-9-(o-tolyl)nona-2,4,6,8-tetraenamide, (E)-N-(2-hydroxy-5-oxocyclopent-1-en-1-yl)-5-(o-tolyl)pent-4-enamide	Streptomyces sp. SANK 60404
BGC0002274.2	0.65	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002277.2	0.65	NRPS	atromentin	Suillus grevillei
BGC0002193.2	0.64	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002174.2	0.59	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0000867.3	0.51	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000231.5	0.49	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002008.3	0.49	other (other)	aryl polyenes	Xenorhabdus doucetiae

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 28832:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 26722:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mbg0: mal

PKS_AT (8..297): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 54.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 66.1
Methoxymalonyl-CoA: 39.2
Methylmalonyl-CoA: 38.7
Propionyl-CoA: 32.6
inactive: 21.6
2-Methylbutyryl-CoA: 21.3
Ethylmalonyl-CoA: 19.6
trans-1,2-CPDA: 18.0
CHC-CoA: 14.4
fatty_acid: 8.4
Acetyl-CoA: 7.0
Isobutyryl-CoA: 4.7
Benzoyl-CoA: 1.9
3-Methylbutyryl-CoA: 0.0

orf3: X

AMP-binding (21..422): X

nrpys: Ppa

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VALFIGAAGK
Nearest Stachelhaus code(s):: Ppa VALFIGAAGK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor