BGC0002497 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002497 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,hglE-KS	1	131,383	High	hexacosalactone A	other:other

BGC0002497 - Region 1 - T1PKS,hglE-KS

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Location: 1 - 131,383 nt. (total: 131,383 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 131,384
Label		BGC0002497

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

hcl15: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

hcl27: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

hcl30: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

hcl8: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: acylase glycosyltransferase methyltransferase

hcl12: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

hcl14: methyltransferase

smcogs: SMCOG1089: methyltransferase

hcl23: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase

hcl22: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

hcl19: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hcl20: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

hcl3_rename1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hclA: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

hclB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

hclD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

hclX: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

hclY: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

hclZ: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002497.3	1.29	other (other)	hexacosalactone A	Streptomyces sp.
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.73	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.66	PKS	oronofacic acid	Pseudomonas syringae
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.59	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.58	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.56	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002497.3	0.99	other (other)	hexacosalactone A	Streptomyces sp.
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002192.3	0.64	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 213 - 124554:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - pk - pk) + (Me-ohmal - ohmal - pk - ohmal) + (pk - ccmal) + (mal) + (Me-mal) + (Me-ccmal - ccmal - pk)
Non-colinear ordering:: hclA + hclB + hcl3_rename1 + hcl19 + hcl20 + hcl21 + hclD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

hclX: X

AMP-binding (9..412): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TFTMFGGPVK
Nearest Stachelhaus code(s):: Leu DFLYFGGVVK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

hclY: X

AMP-binding (15..416): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SGATSGFGIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

hcl12: pk

PKS_AT (12..278): pk

ATSignature: (unknown)

No matches above 50%

minowa: 2-Methylbutyryl-CoA

Prediction, score:

2-Methylbutyryl-CoA: 7.5
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

hclA: pk - mal - pk - pk

PKS_AT (560..858): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 210.7
Methylmalonyl-CoA: 205.8
2-Methylbutyryl-CoA: 185.4
Methoxymalonyl-CoA: 168.1
trans-1,2-CPDA: 134.1
Propionyl-CoA: 128.3
CHC-CoA: 114.6
Malonyl-CoA: 94.9
Isobutyryl-CoA: 91.4
Benzoyl-CoA: 67.6
Acetyl-CoA: 51.7
3-Methylbutyryl-CoA: 43.7
fatty_acid: 34.6
inactive: 24.5

PKS_AT (1585..1870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.1
inactive: 121.2
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 46.5
Propionyl-CoA: 34.7
2-Methylbutyryl-CoA: 16.3
Isobutyryl-CoA: 15.7
Acetyl-CoA: 11.1
Benzoyl-CoA: 9.3
Ethylmalonyl-CoA: 7.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3070..3368): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 231.8
Methylmalonyl-CoA: 225.7
Methoxymalonyl-CoA: 182.8
2-Methylbutyryl-CoA: 167.8
trans-1,2-CPDA: 134.5
Propionyl-CoA: 129.3
CHC-CoA: 108.9
Malonyl-CoA: 108.5
Isobutyryl-CoA: 73.9
Benzoyl-CoA: 62.8
Acetyl-CoA: 38.0
fatty_acid: 36.5
inactive: 24.1
3-Methylbutyryl-CoA: 21.8

PKS_AT (4404..4698): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 229.1
Methylmalonyl-CoA: 224.9
Methoxymalonyl-CoA: 180.2
2-Methylbutyryl-CoA: 160.8
trans-1,2-CPDA: 128.6
Propionyl-CoA: 118.4
CHC-CoA: 113.9
Malonyl-CoA: 104.0
Isobutyryl-CoA: 80.1
Benzoyl-CoA: 67.0
Acetyl-CoA: 40.0
fatty_acid: 38.3
3-Methylbutyryl-CoA: 32.4
inactive: 27.4

hclB: mmal - mal - pk - mal

PKS_AT (576..874): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 225.9
Ethylmalonyl-CoA: 224.0
Methoxymalonyl-CoA: 181.5
2-Methylbutyryl-CoA: 181.3
Propionyl-CoA: 133.7
trans-1,2-CPDA: 129.8
CHC-CoA: 115.2
Malonyl-CoA: 103.6
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 65.0
fatty_acid: 41.2
Acetyl-CoA: 39.9
3-Methylbutyryl-CoA: 36.3
inactive: 27.0

PKS_AT (2150..2441): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.0
inactive: 118.6
Methoxymalonyl-CoA: 56.3
Methylmalonyl-CoA: 44.6
Propionyl-CoA: 31.4
Isobutyryl-CoA: 20.2
2-Methylbutyryl-CoA: 14.9
Benzoyl-CoA: 12.7
Ethylmalonyl-CoA: 11.0
Acetyl-CoA: 10.1
fatty_acid: 7.2
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3708..4006): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 233.5
Methylmalonyl-CoA: 232.1
Methoxymalonyl-CoA: 187.6
2-Methylbutyryl-CoA: 169.3
trans-1,2-CPDA: 149.9
Propionyl-CoA: 130.3
Malonyl-CoA: 112.5
CHC-CoA: 112.2
Isobutyryl-CoA: 78.8
Benzoyl-CoA: 71.1
Acetyl-CoA: 45.0
fatty_acid: 37.4
3-Methylbutyryl-CoA: 27.5
inactive: 25.5

PKS_AT (5498..5787): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.1
inactive: 121.6
Methoxymalonyl-CoA: 59.6
Methylmalonyl-CoA: 47.9
Propionyl-CoA: 33.8
2-Methylbutyryl-CoA: 15.5
Isobutyryl-CoA: 15.0
Acetyl-CoA: 12.1
Benzoyl-CoA: 9.3
Ethylmalonyl-CoA: 3.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

hcl3_rename1: pk - mal

PKS_AT (583..877): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 230.0
Methylmalonyl-CoA: 228.0
Methoxymalonyl-CoA: 181.5
2-Methylbutyryl-CoA: 159.2
trans-1,2-CPDA: 134.3
Propionyl-CoA: 118.4
CHC-CoA: 117.7
Malonyl-CoA: 99.5
Isobutyryl-CoA: 86.4
Benzoyl-CoA: 71.4
Acetyl-CoA: 38.1
3-Methylbutyryl-CoA: 35.6
fatty_acid: 31.6
inactive: 24.3

PKS_AT (2346..2634): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.7
inactive: 115.4
Methoxymalonyl-CoA: 52.1
Methylmalonyl-CoA: 44.1
Propionyl-CoA: 32.4
Isobutyryl-CoA: 21.9
Acetyl-CoA: 13.1
Benzoyl-CoA: 12.3
2-Methylbutyryl-CoA: 7.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

hclD: mmal - mal - pk

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 219.3
Ethylmalonyl-CoA: 217.3
Methoxymalonyl-CoA: 175.2
2-Methylbutyryl-CoA: 148.7
trans-1,2-CPDA: 131.6
CHC-CoA: 105.7
Propionyl-CoA: 104.0
Malonyl-CoA: 85.4
Isobutyryl-CoA: 77.2
Benzoyl-CoA: 60.9
Acetyl-CoA: 35.1
3-Methylbutyryl-CoA: 34.0
fatty_acid: 28.7
inactive: 22.3

PKS_AT (2351..2638): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 232.0
inactive: 114.8
Methoxymalonyl-CoA: 63.4
Methylmalonyl-CoA: 54.9
Propionyl-CoA: 39.0
2-Methylbutyryl-CoA: 21.0
Isobutyryl-CoA: 16.6
Acetyl-CoA: 13.5
Benzoyl-CoA: 12.5
Ethylmalonyl-CoA: 11.4
fatty_acid: 8.4
3-Methylbutyryl-CoA: 6.9
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0

PKS_AT (4205..4503): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 235.0
Methylmalonyl-CoA: 229.2
Methoxymalonyl-CoA: 186.5
2-Methylbutyryl-CoA: 161.6
trans-1,2-CPDA: 138.5
Propionyl-CoA: 124.2
CHC-CoA: 114.3
Malonyl-CoA: 107.7
Isobutyryl-CoA: 77.6
Benzoyl-CoA: 72.0
Acetyl-CoA: 47.9
fatty_acid: 33.5
3-Methylbutyryl-CoA: 31.4
inactive: 30.7

hcl19: mal

PKS_AT (1274..1566): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 58.3%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 73.6
Methoxymalonyl-CoA: 35.1
Methylmalonyl-CoA: 32.7
inactive: 24.4
Propionyl-CoA: 18.8
CHC-CoA: 10.2
Isobutyryl-CoA: 7.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

hcl20: mmal

PKS_AT (631..843): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 8.5
Malonyl-CoA: 4.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor