BGC0002754 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0002754 (Moorena producens JHB)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	63,187	High	hectoramide B	NRPS:Type I

BGC0002754 - Region 1 - NRPS

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Location: 1 - 63,187 nt. (total: 63,187 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 63,188
Label		BGC0002754

Tailoring functions

Transferases: glycosyltransferase

BJP36_15390: sugar transferase

smcogs: SMCOG1146: sugar transferase

Ligases: ligase

BJP36_15450: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BJP36_15455: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BJP36_39125: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BJP36_39130: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Translocases: transporter

BJP36_17805: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002754.2	0.96	NRPS	hectoramide B	Moorena producens JHB
BGC0000465.4	0.70	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0000416.5	0.67	NRPS	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002754.2	0.96	NRPS	hectoramide B	Moorena producens JHB
BGC0000465.4	0.70	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0000416.5	0.67	NRPS	rhabdopeptide 1, rhabdopeptide 2, rhabdopeptide 3, rhabdopeptide 4	Xenorhabdus nematophila ATCC 19061
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 63187:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-NMe-Tyr) + (NMe-Val) + (NMe-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BJP36_15450: Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (534..926): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BJP36_15455: Ser - fatty_acid

Search NORINE for peptide: strict or relaxed

AMP-binding (535..914): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVLHLSFGEK
Nearest Stachelhaus code(s):: Ala DVWHLSLIEK (50% 8Å match); Dpr DVRHLSMVEK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

CAL_domain (1127..1543): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 38.8
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

BJP36_39125: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (544..945): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code(s):: Val DALWLGGTFK (88% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

BJP36_39130: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (543..937): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DALWLGGTFK
Nearest Stachelhaus code(s):: Val DALWLGGTFK (88% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor