BGC0000042 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000042 (Streptomyces sp. MJ635-86F5)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	105,855	hitachimycin	Polyketide	77%

BGC0000042 - Region 1 - T1PKS

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Location: 1 - 105,855 nt. (total: 105,855 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 105,856
Label		BGC0000042

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000042.1	0.80	Polyketide	cremimycin	Streptomyces sp. MJ635-86F5
BGC0000041.1	0.40	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001647.1	0.34	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001283.1	0.31	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0002031.1	0.31	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001544.1	0.31	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561
BGC0001273.1	0.31	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001913.1	0.30	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001276.1	0.30	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.30	Polyketide	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000042.1	0.93	Polyketide	cremimycin	Streptomyces sp. MJ635-86F5
BGC0000041.1	0.72	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001647.1	0.67	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001283.1	0.65	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0002031.1	0.64	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001544.1	0.64	NRP, Polyketide	chlorflavonin	Aspergillus campestris IBT 28561
BGC0001273.1	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001913.1	0.64	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 105855:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - redmal - redmal) + (mal - redmal) + (ohmal - mal) + (ohmal) + (ccmal - Me-ccmal - ccmal) + (ccmal)
Non-colinear ordering:: cmiP4 + cmiP1 + cmiP5 + cmiP8 + cmiP7 + cmiP6

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

cmiP1

PKS_AT (689..976): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.1
inactive: 112.0
Methoxymalonyl-CoA: 62.7
Methylmalonyl-CoA: 56.8
Propionyl-CoA: 52.3
2-Methylbutyryl-CoA: 34.8
Isobutyryl-CoA: 27.2
Ethylmalonyl-CoA: 23.9
Acetyl-CoA: 15.6
Benzoyl-CoA: 15.0
trans-1,2-CPDA: 10.9
CHC-CoA: 3.1
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2020..2307): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.5
inactive: 116.4
Methoxymalonyl-CoA: 59.1
Methylmalonyl-CoA: 52.0
Propionyl-CoA: 32.5
Acetyl-CoA: 24.2
Isobutyryl-CoA: 23.7
Benzoyl-CoA: 22.5
Ethylmalonyl-CoA: 20.0
CHC-CoA: 17.1
2-Methylbutyryl-CoA: 14.1
fatty_acid: 13.3
trans-1,2-CPDA: 10.7
3-Methylbutyryl-CoA: 0.0

cmiP3

PKS_AT (563..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.5
inactive: 88.1
Methoxymalonyl-CoA: 67.2
Methylmalonyl-CoA: 61.6
Propionyl-CoA: 33.7
Acetyl-CoA: 23.8
Isobutyryl-CoA: 22.6
fatty_acid: 17.5
2-Methylbutyryl-CoA: 16.8
CHC-CoA: 16.6
trans-1,2-CPDA: 11.8
Benzoyl-CoA: 11.1
Ethylmalonyl-CoA: 9.5
3-Methylbutyryl-CoA: 0.0

cmiP4

PKS_AT (642..940): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.9
Ethylmalonyl-CoA: 190.5
Methoxymalonyl-CoA: 169.4
2-Methylbutyryl-CoA: 140.8
trans-1,2-CPDA: 108.5
Propionyl-CoA: 101.0
Malonyl-CoA: 93.6
CHC-CoA: 92.4
Isobutyryl-CoA: 74.0
Benzoyl-CoA: 73.6
3-Methylbutyryl-CoA: 52.8
fatty_acid: 38.5
Acetyl-CoA: 37.2
inactive: 28.1

PKS_AT (1650..1924): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.5
inactive: 88.1
Methoxymalonyl-CoA: 67.2
Methylmalonyl-CoA: 61.6
Propionyl-CoA: 33.7
Acetyl-CoA: 23.8
Isobutyryl-CoA: 22.6
fatty_acid: 17.5
2-Methylbutyryl-CoA: 16.8
CHC-CoA: 16.6
trans-1,2-CPDA: 11.8
Benzoyl-CoA: 11.1
Ethylmalonyl-CoA: 9.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (3727..4002): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.5
inactive: 70.7
Methoxymalonyl-CoA: 69.9
Methylmalonyl-CoA: 55.8
Propionyl-CoA: 39.4
Isobutyryl-CoA: 27.0
Ethylmalonyl-CoA: 25.4
Acetyl-CoA: 18.5
Benzoyl-CoA: 9.1
fatty_acid: 8.1
2-Methylbutyryl-CoA: 7.9
CHC-CoA: 7.4
trans-1,2-CPDA: 7.0
3-Methylbutyryl-CoA: 6.7

cmiS3

Search NORINE for peptide: strict or relaxed

AMP-binding (12..409): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DcLFFaLltK
Nearest Stachelhaus code:: leu
Stachelhaus code match:: 60% (weak)

cmiS5

PKS_AT (36..272): pk

ATSignature: (unknown)

No matches above 50%

minowa: Propionyl-CoA

Prediction, score:

Propionyl-CoA: 9.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

cmiS6

Search NORINE for peptide: strict or relaxed

AMP-binding (22..430): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DfgHLSLaaK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 60% (weak)

cmiP5

PKS_AT (565..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.8
inactive: 109.8
Methoxymalonyl-CoA: 60.0
Methylmalonyl-CoA: 54.1
Propionyl-CoA: 33.2
Isobutyryl-CoA: 22.7
Benzoyl-CoA: 21.0
Ethylmalonyl-CoA: 20.6
Acetyl-CoA: 20.0
2-Methylbutyryl-CoA: 18.4
fatty_acid: 14.9
CHC-CoA: 11.5
trans-1,2-CPDA: 11.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2122..2410): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.9
inactive: 109.3
Methoxymalonyl-CoA: 65.9
Methylmalonyl-CoA: 58.7
Propionyl-CoA: 38.1
Ethylmalonyl-CoA: 27.9
CHC-CoA: 24.9
Acetyl-CoA: 24.4
Benzoyl-CoA: 20.8
2-Methylbutyryl-CoA: 20.8
fatty_acid: 19.0
Isobutyryl-CoA: 15.2
trans-1,2-CPDA: 14.5
3-Methylbutyryl-CoA: 0.0

cmiP6

PKS_AT (551..840): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.8
inactive: 119.7
Methoxymalonyl-CoA: 59.8
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 28.9
Isobutyryl-CoA: 25.5
Benzoyl-CoA: 23.5
Ethylmalonyl-CoA: 20.5
Acetyl-CoA: 17.7
fatty_acid: 15.8
2-Methylbutyryl-CoA: 13.1
CHC-CoA: 12.8
trans-1,2-CPDA: 9.5
3-Methylbutyryl-CoA: 0.0

cmiP7

PKS_AT (572..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.8
inactive: 116.9
Methoxymalonyl-CoA: 60.9
Methylmalonyl-CoA: 54.3
Propionyl-CoA: 27.1
Isobutyryl-CoA: 26.0
Ethylmalonyl-CoA: 24.7
Benzoyl-CoA: 24.1
Acetyl-CoA: 24.1
CHC-CoA: 14.8
2-Methylbutyryl-CoA: 14.6
fatty_acid: 11.3
trans-1,2-CPDA: 10.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2265..2563): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.5
Ethylmalonyl-CoA: 192.6
Methoxymalonyl-CoA: 166.4
2-Methylbutyryl-CoA: 129.5
trans-1,2-CPDA: 103.9
Propionyl-CoA: 100.7
CHC-CoA: 97.7
Malonyl-CoA: 93.9
Benzoyl-CoA: 66.8
Isobutyryl-CoA: 66.4
3-Methylbutyryl-CoA: 52.3
Acetyl-CoA: 40.2
fatty_acid: 37.5
inactive: 31.4

PKS_AT (3965..4253): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.1
inactive: 109.0
Methoxymalonyl-CoA: 54.6
Methylmalonyl-CoA: 49.2
Propionyl-CoA: 29.5
Isobutyryl-CoA: 24.0
Acetyl-CoA: 23.9
Benzoyl-CoA: 22.5
Ethylmalonyl-CoA: 18.4
CHC-CoA: 14.5
2-Methylbutyryl-CoA: 13.8
fatty_acid: 13.7
trans-1,2-CPDA: 10.6
3-Methylbutyryl-CoA: 0.0

cmiP8

PKS_AT (561..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.6
inactive: 79.3
Methoxymalonyl-CoA: 57.1
Propionyl-CoA: 43.5
Methylmalonyl-CoA: 39.0
Isobutyryl-CoA: 23.5
Acetyl-CoA: 15.3
fatty_acid: 13.6
2-Methylbutyryl-CoA: 11.2
Ethylmalonyl-CoA: 8.6
Benzoyl-CoA: 6.5
CHC-CoA: 4.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

BAO66547.1

Search NORINE for peptide: strict or relaxed

AMP-binding (27..426): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophilic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: iAngsGGqiK
Nearest Stachelhaus code:: val
Stachelhaus code match:: 40% (weak)