BGC0000097 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000097 (Streptomyces sp. MP39-85)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	89,357	BE-14106	Polyketide:Modular type I	82%

BGC0000097 - Region 1 - T1PKS

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Location: 1 - 89,357 nt. (total: 89,357 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 89,358
Label		BGC0000097

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000097.1	0.77	Polyketide	ML-449	Streptomyces sp. MP39-85
BGC0000029.1	0.46	Polyketide	BE-14106	Streptomyces sp. DSM 21069
BGC0001349.1	0.38	NRP, Polyketide	heronamide A, heronamide B, heronamide C, heronamide D, heronamide E, heronamide F	Streptomyces sp. SCSIO 03032
BGC0000041.1	0.35	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122.1	0.32	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001273.1	0.30	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275.1	0.30	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001304.1	0.30	Polyketide	aflavarin	Aspergillus flavus NRRL3357
BGC0001276.1	0.30	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.30	Polyketide	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000097.1	0.92	Polyketide	ML-449	Streptomyces sp. MP39-85
BGC0000029.1	0.76	Polyketide	BE-14106	Streptomyces sp. DSM 21069
BGC0001349.1	0.70	NRP, Polyketide	heronamide A, heronamide B, heronamide C, heronamide D, heronamide E, heronamide F	Streptomyces sp. SCSIO 03032
BGC0000041.1	0.68	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000122.1	0.65	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001273.1	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275.1	0.63	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001304.1	0.63	Polyketide	aflavarin	Aspergillus flavus NRRL3357
BGC0001276.1	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.63	Polyketide	(-)-Mellein	Parastagonospora nodorum

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 89357:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - ccmal - ccmal - ccmal) + (ccmal - Me-ccmal) + (ccmal - ccmal) + (pk - ccmal) + (ccmal) + (ccmal)
Non-colinear ordering:: mlaA1 + mlaB + mlaD + mlaF + mlaE + mlaG

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

mlaA1

PKS_AT (540..834): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 210.1
Methylmalonyl-CoA: 205.4
Methoxymalonyl-CoA: 163.1
2-Methylbutyryl-CoA: 142.6
Propionyl-CoA: 109.3
trans-1,2-CPDA: 105.5
Malonyl-CoA: 98.6
CHC-CoA: 97.4
Isobutyryl-CoA: 73.5
Benzoyl-CoA: 51.8
Acetyl-CoA: 50.1
3-Methylbutyryl-CoA: 43.4
inactive: 38.9
fatty_acid: 21.5

PKS_AT (1558..1850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.8
inactive: 106.6
Methoxymalonyl-CoA: 64.3
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 33.6
Isobutyryl-CoA: 25.6
Acetyl-CoA: 20.8
2-Methylbutyryl-CoA: 11.7
Benzoyl-CoA: 9.1
CHC-CoA: 4.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -3.0

PKS_AT (3319..3615): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.3
inactive: 101.1
Methoxymalonyl-CoA: 64.7
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 34.5
Isobutyryl-CoA: 25.5
Acetyl-CoA: 22.0
2-Methylbutyryl-CoA: 14.9
Benzoyl-CoA: 13.2
CHC-CoA: 11.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.7

PKS_AT (5092..5384): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.4
inactive: 106.5
Methoxymalonyl-CoA: 66.0
Methylmalonyl-CoA: 60.7
Propionyl-CoA: 34.5
Isobutyryl-CoA: 27.4
Acetyl-CoA: 19.8
2-Methylbutyryl-CoA: 14.3
Benzoyl-CoA: 9.0
CHC-CoA: 6.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.7

mlaA2

PKS_AT (562..852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.0
inactive: 95.9
Methoxymalonyl-CoA: 84.7
Methylmalonyl-CoA: 65.5
Isobutyryl-CoA: 45.2
Propionyl-CoA: 40.7
Ethylmalonyl-CoA: 23.3
fatty_acid: 22.8
Acetyl-CoA: 21.8
2-Methylbutyryl-CoA: 20.6
CHC-CoA: 17.3
Benzoyl-CoA: 14.0
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 0.0

mlaB

PKS_AT (645..938): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.9
inactive: 109.4
Methoxymalonyl-CoA: 65.8
Methylmalonyl-CoA: 49.0
Isobutyryl-CoA: 41.9
Propionyl-CoA: 35.1
Ethylmalonyl-CoA: 33.0
Benzoyl-CoA: 21.1
2-Methylbutyryl-CoA: 16.1
Acetyl-CoA: 14.3
trans-1,2-CPDA: 9.0
CHC-CoA: 8.5
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2335..2633): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.9
Ethylmalonyl-CoA: 208.7
Methoxymalonyl-CoA: 173.1
2-Methylbutyryl-CoA: 149.0
trans-1,2-CPDA: 146.1
Propionyl-CoA: 110.6
Malonyl-CoA: 97.2
CHC-CoA: 86.7
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 77.1
3-Methylbutyryl-CoA: 46.5
Acetyl-CoA: 45.3
inactive: 36.4
fatty_acid: 31.0

mlaJ

Search NORINE for peptide: strict or relaxed

AMP-binding (23..436): X

NRPSPredictor2: gly, ala, val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DffDAsgaaK
Nearest Stachelhaus code:: lys
Stachelhaus code match:: 40% (weak)

mlaK

PKS_AT (4..267): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 11.5
Methylmalonyl-CoA: 9.4
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

mlaL

Search NORINE for peptide: strict or relaxed

AMP-binding (19..405): X

NRPSPredictor2: val, leu, ile, abu, iva

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: val, leu, ile, abu, iva
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DcFFFalVtK
Nearest Stachelhaus code:: ile
Stachelhaus code match:: 60% (weak)

mlaD

PKS_AT (553..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.8
inactive: 97.4
Methylmalonyl-CoA: 54.6
Methoxymalonyl-CoA: 53.5
Propionyl-CoA: 35.9
Isobutyryl-CoA: 24.4
Ethylmalonyl-CoA: 22.2
CHC-CoA: 22.1
Acetyl-CoA: 22.0
Benzoyl-CoA: 19.4
2-Methylbutyryl-CoA: 17.2
fatty_acid: 16.7
trans-1,2-CPDA: 4.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2165..2436): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.2
inactive: 95.1
Methoxymalonyl-CoA: 58.4
Methylmalonyl-CoA: 44.0
Propionyl-CoA: 29.0
Isobutyryl-CoA: 25.7
Benzoyl-CoA: 24.4
Acetyl-CoA: 24.1
Ethylmalonyl-CoA: 16.8
CHC-CoA: 16.1
2-Methylbutyryl-CoA: 13.9
fatty_acid: 11.4
trans-1,2-CPDA: 9.2
3-Methylbutyryl-CoA: 0.0

mlaG

PKS_AT (550..822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.3
inactive: 95.8
Methoxymalonyl-CoA: 61.1
Methylmalonyl-CoA: 53.4
Propionyl-CoA: 29.6
Ethylmalonyl-CoA: 25.0
Acetyl-CoA: 24.8
Isobutyryl-CoA: 22.9
Benzoyl-CoA: 18.6
2-Methylbutyryl-CoA: 18.2
fatty_acid: 16.0
CHC-CoA: 16.0
trans-1,2-CPDA: 15.3
3-Methylbutyryl-CoA: 0.0

mlaF

PKS_AT (558..836): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 224.9
Methylmalonyl-CoA: 211.4
Methoxymalonyl-CoA: 166.2
2-Methylbutyryl-CoA: 156.6
Propionyl-CoA: 122.3
trans-1,2-CPDA: 120.1
CHC-CoA: 106.1
Isobutyryl-CoA: 83.3
Malonyl-CoA: 82.7
Acetyl-CoA: 62.2
Benzoyl-CoA: 60.8
3-Methylbutyryl-CoA: 37.9
fatty_acid: 31.8
inactive: 21.3

PKS_AT (2199..2464): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.9
inactive: 105.8
Methoxymalonyl-CoA: 71.7
Methylmalonyl-CoA: 62.5
Propionyl-CoA: 45.5
Isobutyryl-CoA: 36.5
Acetyl-CoA: 27.0
2-Methylbutyryl-CoA: 23.6
Ethylmalonyl-CoA: 21.5
CHC-CoA: 19.0
Benzoyl-CoA: 15.7
fatty_acid: 14.8
trans-1,2-CPDA: 14.2
3-Methylbutyryl-CoA: 0.0

mlaE

PKS_AT (527..798): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.1
inactive: 97.4
Methoxymalonyl-CoA: 62.0
Methylmalonyl-CoA: 46.0
Propionyl-CoA: 31.2
Benzoyl-CoA: 26.2
Isobutyryl-CoA: 25.1
Acetyl-CoA: 23.7
CHC-CoA: 16.4
Ethylmalonyl-CoA: 14.4
fatty_acid: 11.8
2-Methylbutyryl-CoA: 11.3
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 0.0