BGC0000135 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000135 (Streptomyces sp. SN-593)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	104,853	caniferolide A / caniferolide B / caniferolide C / caniferolide D	Polyketide:Modular type I	11%

BGC0000135 - Region 1 - T1PKS

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Location: 1 - 104,853 nt. (total: 104,853 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 104,854
Label		BGC0000135

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000135.1	0.74	Polyketide	reveromycin A	Streptomyces sp. SN-593
BGC0001164.1	0.31	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067.1	0.31	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000353.1	0.29	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0000261.1	0.29	Polyketide	R1128	Streptomyces sp. R1128
BGC0000122.1	0.29	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001895.1	0.29	Polyketide	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0001162.1	0.29	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001068.1	0.28	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000286.1	0.28	Polyketide	viguiepinol	Streptomyces sp. KO-3988

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000135.1	0.91	Polyketide	reveromycin A	Streptomyces sp. SN-593
BGC0001164.1	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067.1	0.64	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000353.1	0.63	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0000261.1	0.63	Polyketide	R1128	Streptomyces sp. R1128
BGC0000122.1	0.63	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001895.1	0.63	Polyketide	huanglongmycin A, huanglongmycin B, huanglongmycin C	Streptomyces sp. CB09001
BGC0001162.1	0.62	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001068.1	0.62	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0000286.1	0.61	Polyketide	viguiepinol	Streptomyces sp. KO-3988

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 104853:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ccmal - Me-ccmal - ccmal) + (Me-ohmal - redmal - ohmal) + (Me-redmal - ohmal) + (Me-ccmal - ccmal - Me-ohmal - ccmal)
Non-colinear ordering:: revA + revB + revC + revD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

revS

CAL_domain (43..477): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 14.9
NH2: 11.2
Acetyl-CoA: 1.0
shikimic_acid: 0.0
AHBA: -0.5

revB

PKS_AT (579..879): mmal

ATSignature: Methylmalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.6
Ethylmalonyl-CoA: 163.3
Methoxymalonyl-CoA: 147.8
2-Methylbutyryl-CoA: 100.9
CHC-CoA: 96.8
trans-1,2-CPDA: 95.4
Isobutyryl-CoA: 78.4
Propionyl-CoA: 75.3
Malonyl-CoA: 71.4
Benzoyl-CoA: 71.1
3-Methylbutyryl-CoA: 36.9
fatty_acid: 36.2
Acetyl-CoA: 22.5
inactive: 17.2

PKS_AT (2121..2395): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.6
inactive: 96.4
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 50.3
Propionyl-CoA: 28.3
Isobutyryl-CoA: 25.3
Acetyl-CoA: 24.9
Benzoyl-CoA: 20.9
Ethylmalonyl-CoA: 19.9
fatty_acid: 14.9
2-Methylbutyryl-CoA: 14.1
CHC-CoA: 12.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4181..4460): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.4
inactive: 90.5
Methoxymalonyl-CoA: 59.1
Methylmalonyl-CoA: 44.6
Isobutyryl-CoA: 27.0
Acetyl-CoA: 26.1
Propionyl-CoA: 24.7
Ethylmalonyl-CoA: 20.2
Benzoyl-CoA: 19.5
fatty_acid: 13.4
2-Methylbutyryl-CoA: 13.1
CHC-CoA: 9.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

revC

PKS_AT (602..894): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 165.5
2-Methylbutyryl-CoA: 133.3
Propionyl-CoA: 123.4
trans-1,2-CPDA: 122.6
Malonyl-CoA: 118.6
CHC-CoA: 106.1
Isobutyryl-CoA: 81.2
Acetyl-CoA: 71.5
Benzoyl-CoA: 65.6
inactive: 45.6
3-Methylbutyryl-CoA: 36.1
fatty_acid: 25.6

PKS_AT (2704..2982): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.1
inactive: 97.9
Methoxymalonyl-CoA: 69.7
Methylmalonyl-CoA: 53.8
Propionyl-CoA: 35.0
Isobutyryl-CoA: 28.2
Acetyl-CoA: 23.7
Benzoyl-CoA: 22.7
fatty_acid: 22.0
CHC-CoA: 20.6
Ethylmalonyl-CoA: 19.8
trans-1,2-CPDA: 17.7
2-Methylbutyryl-CoA: 9.5
3-Methylbutyryl-CoA: 0.0

revK

PKS_AT (11..275): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

revL

Search NORINE for peptide: strict or relaxed

AMP-binding (91..307): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ---NrSgsyK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 30% (weak)

revA

PKS_AT (546..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.6
Ethylmalonyl-CoA: 206.6
Methoxymalonyl-CoA: 163.5
2-Methylbutyryl-CoA: 150.5
Malonyl-CoA: 116.7
CHC-CoA: 115.3
trans-1,2-CPDA: 107.9
Propionyl-CoA: 107.2
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 63.3
3-Methylbutyryl-CoA: 46.5
Acetyl-CoA: 45.6
fatty_acid: 40.1
inactive: 30.0

PKS_AT (1587..1866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.7
inactive: 86.5
Methoxymalonyl-CoA: 60.0
Methylmalonyl-CoA: 44.6
Isobutyryl-CoA: 27.1
Acetyl-CoA: 26.0
Propionyl-CoA: 24.8
Benzoyl-CoA: 20.2
Ethylmalonyl-CoA: 18.6
fatty_acid: 13.7
2-Methylbutyryl-CoA: 13.6
CHC-CoA: 11.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3338..3628): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 206.9
Methoxymalonyl-CoA: 171.9
2-Methylbutyryl-CoA: 148.3
trans-1,2-CPDA: 127.7
Propionyl-CoA: 113.3
CHC-CoA: 111.3
Malonyl-CoA: 98.5
Isobutyryl-CoA: 76.6
Benzoyl-CoA: 70.9
Acetyl-CoA: 54.3
fatty_acid: 52.7
3-Methylbutyryl-CoA: 33.3
inactive: 29.7

PKS_AT (5034..5313): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.5
inactive: 86.8
Methoxymalonyl-CoA: 61.4
Methylmalonyl-CoA: 46.9
Isobutyryl-CoA: 29.5
Acetyl-CoA: 26.5
Propionyl-CoA: 26.2
Ethylmalonyl-CoA: 23.2
Benzoyl-CoA: 19.7
2-Methylbutyryl-CoA: 15.4
fatty_acid: 13.5
CHC-CoA: 12.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

revD

PKS_AT (577..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.1
Ethylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 171.7
2-Methylbutyryl-CoA: 148.2
trans-1,2-CPDA: 131.0
Propionyl-CoA: 114.2
CHC-CoA: 110.1
Malonyl-CoA: 100.2
Isobutyryl-CoA: 77.9
Benzoyl-CoA: 70.5
Acetyl-CoA: 54.0
fatty_acid: 52.7
3-Methylbutyryl-CoA: 32.7
inactive: 29.5

PKS_AT (2328..2605): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.8
inactive: 91.4
Methoxymalonyl-CoA: 58.9
Methylmalonyl-CoA: 48.5
Propionyl-CoA: 27.5
Acetyl-CoA: 25.9
Isobutyryl-CoA: 25.4
Benzoyl-CoA: 20.9
Ethylmalonyl-CoA: 19.8
2-Methylbutyryl-CoA: 14.9
fatty_acid: 14.0
CHC-CoA: 12.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4055..4345): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.0
Ethylmalonyl-CoA: 205.5
Methoxymalonyl-CoA: 172.1
2-Methylbutyryl-CoA: 144.1
trans-1,2-CPDA: 130.6
Propionyl-CoA: 115.5
CHC-CoA: 108.2
Malonyl-CoA: 99.9
Isobutyryl-CoA: 72.9
Benzoyl-CoA: 68.4
Acetyl-CoA: 56.8
fatty_acid: 50.4
3-Methylbutyryl-CoA: 36.6
inactive: 32.5

PKS_AT (5564..5843): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.9
inactive: 88.6
Methoxymalonyl-CoA: 59.1
Methylmalonyl-CoA: 44.6
Isobutyryl-CoA: 29.0
Acetyl-CoA: 26.0
Propionyl-CoA: 23.6
Benzoyl-CoA: 19.3
Ethylmalonyl-CoA: 18.6
2-Methylbutyryl-CoA: 14.9
fatty_acid: 13.4
CHC-CoA: 12.3
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0