BGC0001348 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001348 (Streptomyces varsoviensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	70,801	bafilomycin B1	Polyketide:Modular type I	83%

BGC0001348 - Region 1 - T1PKS

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Location: 1 - 70,801 nt. (total: 70,801 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 70,802
Label		BGC0001348

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001348.1	0.71	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000041.1	0.40	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000018.1	0.37	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000028.1	0.34	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0001273.1	0.31	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.31	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.31	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001164.1	0.31	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001275.1	0.30	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001504.1	0.30	Polyketide	amycomicin	Amycolatopsis sp. AA4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001348.1	0.89	Polyketide	JBIR-100	Streptomyces varsoviensis
BGC0000041.1	0.72	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000018.1	0.69	Polyketide	angolamycin	Streptomyces eurythermus
BGC0000028.1	0.67	Polyketide	bafilomycin B1	Streptomyces lohii
BGC0001273.1	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001164.1	0.64	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001275.1	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001504.1	0.63	Polyketide	amycomicin	Amycolatopsis sp. AA4

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 70801:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (emal) + (Me-ohmal) + (Me-ohmal - ohmal - Me-mal) + (Me-ohmal - ohemal - ccmal) + (pk - pk) + (Me-ohmal - pk) + (pk)
Non-colinear ordering:: IF55_RS40995 + IF55_RS36525 + IF55_RS32365 + IF55_RS32370 + IF55_RS32375 + IF55_RS41000 + IF55_RS32385

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

IF55_RS40995

PKS_AT (156..453): emal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 83.3%; Methoxymalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 155.3
2-Methylbutyryl-CoA: 151.9
Methylmalonyl-CoA: 149.9
Methoxymalonyl-CoA: 126.1
Propionyl-CoA: 124.0
CHC-CoA: 89.7
trans-1,2-CPDA: 86.4
Malonyl-CoA: 79.7
Isobutyryl-CoA: 73.0
Benzoyl-CoA: 45.0
3-Methylbutyryl-CoA: 29.9
Acetyl-CoA: 27.9
inactive: 19.3
fatty_acid: 11.5

IF55_RS32365

PKS_AT (1..187): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 108.9
Ethylmalonyl-CoA: 107.1
Methoxymalonyl-CoA: 79.8
2-Methylbutyryl-CoA: 55.8
Malonyl-CoA: 52.1
trans-1,2-CPDA: 51.1
Propionyl-CoA: 42.2
Isobutyryl-CoA: 42.2
Benzoyl-CoA: 39.7
CHC-CoA: 28.4
3-Methylbutyryl-CoA: 22.6
Acetyl-CoA: 19.6
fatty_acid: 15.6
inactive: 12.1

PKS_AT (1503..1806): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.5
inactive: 114.3
Methoxymalonyl-CoA: 66.9
Methylmalonyl-CoA: 56.5
Propionyl-CoA: 44.2
Isobutyryl-CoA: 32.8
Ethylmalonyl-CoA: 18.4
2-Methylbutyryl-CoA: 16.9
Acetyl-CoA: 16.4
CHC-CoA: 14.4
Benzoyl-CoA: 14.3
fatty_acid: 9.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3221..3517): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 198.1
Ethylmalonyl-CoA: 196.6
Methoxymalonyl-CoA: 157.6
2-Methylbutyryl-CoA: 139.9
trans-1,2-CPDA: 129.5
CHC-CoA: 111.0
Propionyl-CoA: 103.0
Malonyl-CoA: 77.8
Benzoyl-CoA: 73.0
Isobutyryl-CoA: 67.6
3-Methylbutyryl-CoA: 42.3
Acetyl-CoA: 37.7
fatty_acid: 26.4
inactive: 25.6

IF55_RS32370

PKS_AT (611..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 167.4
Methoxymalonyl-CoA: 142.8
Propionyl-CoA: 96.9
CHC-CoA: 82.7
Ethylmalonyl-CoA: 79.5
2-Methylbutyryl-CoA: 78.1
Isobutyryl-CoA: 77.3
trans-1,2-CPDA: 54.4
Benzoyl-CoA: 44.5
Malonyl-CoA: 41.9
Acetyl-CoA: 22.1
3-Methylbutyryl-CoA: 17.0
fatty_acid: 14.1
inactive: 0.0

PKS_AT (2166..2463): emal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 95.8%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 221.1
Methylmalonyl-CoA: 207.0
Methoxymalonyl-CoA: 183.2
2-Methylbutyryl-CoA: 136.3
trans-1,2-CPDA: 128.1
CHC-CoA: 111.5
Propionyl-CoA: 99.5
Malonyl-CoA: 92.8
Benzoyl-CoA: 74.6
Isobutyryl-CoA: 70.3
3-Methylbutyryl-CoA: 38.1
Acetyl-CoA: 30.9
fatty_acid: 25.5
inactive: 25.0

PKS_AT (3754..4054): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.8
inactive: 93.4
Methoxymalonyl-CoA: 60.4
Methylmalonyl-CoA: 49.2
Propionyl-CoA: 29.7
Ethylmalonyl-CoA: 25.6
Acetyl-CoA: 20.8
2-Methylbutyryl-CoA: 19.5
CHC-CoA: 17.6
Isobutyryl-CoA: 14.5
Benzoyl-CoA: 13.3
fatty_acid: 8.5
trans-1,2-CPDA: 8.3
3-Methylbutyryl-CoA: 0.0

IF55_RS32375

PKS_AT (566..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 230.1
Methylmalonyl-CoA: 229.7
Methoxymalonyl-CoA: 188.4
2-Methylbutyryl-CoA: 172.0
trans-1,2-CPDA: 144.2
CHC-CoA: 132.3
Propionyl-CoA: 126.0
Malonyl-CoA: 115.6
Isobutyryl-CoA: 103.2
Benzoyl-CoA: 101.1
Acetyl-CoA: 56.9
3-Methylbutyryl-CoA: 43.9
inactive: 40.2
fatty_acid: 35.9

PKS_AT (2328..2588): pk

ATSignature: (unknown)

No matches above 50%

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 28.5
fatty_acid: 18.9
Ethylmalonyl-CoA: 13.0
2-Methylbutyryl-CoA: 12.5
Methylmalonyl-CoA: 12.2
Methoxymalonyl-CoA: 9.5
Propionyl-CoA: 8.3
trans-1,2-CPDA: 4.8
CHC-CoA: 3.8
Isobutyryl-CoA: 2.8
Malonyl-CoA: 2.4
Benzoyl-CoA: 2.0
inactive: 0.0
3-Methylbutyryl-CoA: -3.0

IF55_RS41000

PKS_AT (566..869): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.2
Ethylmalonyl-CoA: 222.6
Methoxymalonyl-CoA: 182.4
2-Methylbutyryl-CoA: 153.6
Malonyl-CoA: 114.4
trans-1,2-CPDA: 112.5
Propionyl-CoA: 111.7
Isobutyryl-CoA: 107.2
CHC-CoA: 95.9
Benzoyl-CoA: 81.8
Acetyl-CoA: 66.8
inactive: 46.9
3-Methylbutyryl-CoA: 42.8
fatty_acid: 30.9

PKS_AT (2171..2468): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 224.9
Methylmalonyl-CoA: 218.5
Methoxymalonyl-CoA: 177.6
2-Methylbutyryl-CoA: 153.4
trans-1,2-CPDA: 115.3
CHC-CoA: 107.7
Propionyl-CoA: 107.6
Malonyl-CoA: 86.1
Isobutyryl-CoA: 81.8
Benzoyl-CoA: 66.0
Acetyl-CoA: 56.6
3-Methylbutyryl-CoA: 31.0
fatty_acid: 26.2
inactive: 21.2

IF55_RS36525

PKS_AT (561..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 223.2
Ethylmalonyl-CoA: 222.6
Methoxymalonyl-CoA: 182.4
2-Methylbutyryl-CoA: 153.6
Malonyl-CoA: 114.4
trans-1,2-CPDA: 112.5
Propionyl-CoA: 111.7
Isobutyryl-CoA: 107.2
CHC-CoA: 95.9
Benzoyl-CoA: 81.8
Acetyl-CoA: 66.8
inactive: 46.9
3-Methylbutyryl-CoA: 42.8
fatty_acid: 30.9

IF55_RS32385

PKS_AT (566..862): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%; Methoxymalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 134.8
Methoxymalonyl-CoA: 134.3
Ethylmalonyl-CoA: 117.3
2-Methylbutyryl-CoA: 94.3
Malonyl-CoA: 93.3
Propionyl-CoA: 89.4
trans-1,2-CPDA: 87.5
CHC-CoA: 68.9
Isobutyryl-CoA: 49.7
fatty_acid: 44.8
inactive: 26.4
Acetyl-CoA: 25.3
Benzoyl-CoA: 25.1
3-Methylbutyryl-CoA: 14.3

IF55_RS32430

PKS_AT (15..276): mxmal

ATSignature: (unknown)

No matches above 50%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 13.0
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

IF55_RS32435

Search NORINE for peptide: strict or relaxed

AMP-binding (103..316): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: tnlNVAMlnK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 50% (weak)