BGC0000021 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000021 (Amycolatopsis sp. FU40)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	115,746	JBIR-100	Polyketide:Modular type I	72%

BGC0000021 - Region 1 - T1PKS

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Location: 1 - 115,746 nt. (total: 115,746 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 115,747
Label		BGC0000021

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000021.1	0.77	Polyketide	apoptolidin	Nocardiopsis sp. FU 40
BGC0000041.1	0.37	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273.1	0.36	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.35	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.35	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.35	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001909.1	0.32	Polyketide	strobilurin	Strobilurus tenacellus
BGC0000170.1	0.32	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0000353.1	0.32	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0000003.1	0.32	Polyketide	AF-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000021.1	0.93	Polyketide	apoptolidin	Nocardiopsis sp. FU 40
BGC0000041.1	0.70	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001273.1	0.68	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276.1	0.68	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.1	0.68	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275.1	0.68	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001909.1	0.66	Polyketide	strobilurin	Strobilurus tenacellus
BGC0000170.1	0.65	Polyketide	yanuthone D	Aspergillus niger ATCC 1015
BGC0000353.1	0.65	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0000003.1	0.65	Polyketide	AF-toxin	Alternaria alternata

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 3921 - 115746:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal) + (ohmal - redmal - pk) + (ccmal - pk) + (Me-ccmal - Me-ccmal) + (ccmal) + (pk - ccmal - Me-ohmal - Me-ohmal) + (Me-ccmal)
Non-colinear ordering:: apoS4 + apoS5 + apoS6 + apoS7 + apoS2 + apoS1 + apoS8

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

apoS10

PKS_AT (488..785): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.2
Ethylmalonyl-CoA: 194.1
Methoxymalonyl-CoA: 161.2
2-Methylbutyryl-CoA: 127.7
trans-1,2-CPDA: 119.4
CHC-CoA: 106.5
Malonyl-CoA: 89.2
Propionyl-CoA: 89.0
Isobutyryl-CoA: 72.1
Benzoyl-CoA: 52.8
Acetyl-CoA: 45.9
3-Methylbutyryl-CoA: 32.2
inactive: 31.6
fatty_acid: 13.4

apoS8

PKS_AT (371..662): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.9
Ethylmalonyl-CoA: 215.5
Methoxymalonyl-CoA: 174.2
2-Methylbutyryl-CoA: 157.8
trans-1,2-CPDA: 146.6
Propionyl-CoA: 118.3
Malonyl-CoA: 109.3
CHC-CoA: 102.4
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 73.2
Acetyl-CoA: 61.3
fatty_acid: 44.9
inactive: 29.8
3-Methylbutyryl-CoA: 29.4

apoS7

PKS_AT (559..848): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.6
Ethylmalonyl-CoA: 214.8
Methoxymalonyl-CoA: 173.2
2-Methylbutyryl-CoA: 160.4
trans-1,2-CPDA: 136.6
Propionyl-CoA: 119.1
CHC-CoA: 108.1
Malonyl-CoA: 103.4
Isobutyryl-CoA: 80.1
Benzoyl-CoA: 68.5
Acetyl-CoA: 54.1
3-Methylbutyryl-CoA: 44.5
fatty_acid: 37.4
inactive: 26.9

PKS_AT (2259..2556): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 218.6
Ethylmalonyl-CoA: 217.0
Methoxymalonyl-CoA: 170.2
2-Methylbutyryl-CoA: 150.0
trans-1,2-CPDA: 148.3
Propionyl-CoA: 127.2
Malonyl-CoA: 112.1
CHC-CoA: 107.0
Isobutyryl-CoA: 83.4
Benzoyl-CoA: 56.8
Acetyl-CoA: 51.7
fatty_acid: 41.9
inactive: 38.9
3-Methylbutyryl-CoA: 35.2

apoS6

PKS_AT (541..820): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.9
inactive: 100.9
Methoxymalonyl-CoA: 65.4
Methylmalonyl-CoA: 59.6
Propionyl-CoA: 42.7
Benzoyl-CoA: 28.1
Acetyl-CoA: 26.7
Ethylmalonyl-CoA: 25.9
2-Methylbutyryl-CoA: 22.8
Isobutyryl-CoA: 21.0
trans-1,2-CPDA: 14.6
CHC-CoA: 12.7
fatty_acid: 12.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2274..2564): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 217.2
Methylmalonyl-CoA: 214.6
Methoxymalonyl-CoA: 172.9
2-Methylbutyryl-CoA: 159.8
trans-1,2-CPDA: 137.4
Propionyl-CoA: 116.6
Malonyl-CoA: 102.4
CHC-CoA: 93.7
Isobutyryl-CoA: 84.7
Benzoyl-CoA: 71.5
Acetyl-CoA: 59.2
3-Methylbutyryl-CoA: 33.7
fatty_acid: 29.9
inactive: 25.3

apoS5

PKS_AT (543..822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.4
inactive: 96.9
Methoxymalonyl-CoA: 59.1
Methylmalonyl-CoA: 50.6
Propionyl-CoA: 44.4
Benzoyl-CoA: 22.6
Isobutyryl-CoA: 17.3
Acetyl-CoA: 13.7
2-Methylbutyryl-CoA: 11.7
trans-1,2-CPDA: 11.3
fatty_acid: 10.2
CHC-CoA: 8.9
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -0.7

PKS_AT (1973..2264): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.7
inactive: 121.6
Methoxymalonyl-CoA: 73.4
Methylmalonyl-CoA: 66.9
Isobutyryl-CoA: 49.9
Propionyl-CoA: 47.8
Ethylmalonyl-CoA: 33.5
2-Methylbutyryl-CoA: 32.9
Benzoyl-CoA: 31.8
Acetyl-CoA: 30.4
CHC-CoA: 23.4
fatty_acid: 18.8
trans-1,2-CPDA: 14.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (4011..4301): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 227.0
Methylmalonyl-CoA: 221.8
Methoxymalonyl-CoA: 176.7
2-Methylbutyryl-CoA: 160.9
trans-1,2-CPDA: 142.5
Propionyl-CoA: 118.3
Malonyl-CoA: 111.2
CHC-CoA: 97.4
Isobutyryl-CoA: 87.9
Benzoyl-CoA: 74.8
Acetyl-CoA: 66.2
fatty_acid: 35.7
3-Methylbutyryl-CoA: 35.7
inactive: 26.8

apoS4

PKS_AT (77..354): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.3
inactive: 97.7
Methoxymalonyl-CoA: 61.0
Methylmalonyl-CoA: 55.7
Propionyl-CoA: 41.6
Ethylmalonyl-CoA: 25.7
Benzoyl-CoA: 24.5
Isobutyryl-CoA: 19.1
Acetyl-CoA: 17.7
2-Methylbutyryl-CoA: 15.5
fatty_acid: 14.7
trans-1,2-CPDA: 13.1
CHC-CoA: 12.7
3-Methylbutyryl-CoA: 0.0

apoS2

PKS_AT (554..831): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.0
inactive: 100.3
Methoxymalonyl-CoA: 59.0
Methylmalonyl-CoA: 55.7
Propionyl-CoA: 39.7
Ethylmalonyl-CoA: 28.7
Benzoyl-CoA: 24.5
Isobutyryl-CoA: 20.4
2-Methylbutyryl-CoA: 18.8
Acetyl-CoA: 17.9
fatty_acid: 14.6
trans-1,2-CPDA: 13.2
CHC-CoA: 12.7
3-Methylbutyryl-CoA: 0.0

apoS1

PKS_AT (549..845): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 95.8%; Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 192.8
Methylmalonyl-CoA: 191.6
Methoxymalonyl-CoA: 176.8
Malonyl-CoA: 125.6
2-Methylbutyryl-CoA: 124.5
trans-1,2-CPDA: 115.9
Propionyl-CoA: 112.4
Isobutyryl-CoA: 94.7
CHC-CoA: 91.1
Acetyl-CoA: 65.4
Benzoyl-CoA: 59.2
3-Methylbutyryl-CoA: 53.2
fatty_acid: 36.6
inactive: 36.3

PKS_AT (2036..2330): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.3
inactive: 117.5
Methoxymalonyl-CoA: 59.8
Methylmalonyl-CoA: 56.9
Isobutyryl-CoA: 27.7
Propionyl-CoA: 26.2
fatty_acid: 22.4
Benzoyl-CoA: 20.0
Ethylmalonyl-CoA: 15.6
Acetyl-CoA: 12.4
CHC-CoA: 5.2
2-Methylbutyryl-CoA: 4.2
3-Methylbutyryl-CoA: 2.9
trans-1,2-CPDA: 0.0

PKS_AT (3724..4013): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.5
Ethylmalonyl-CoA: 213.4
Methoxymalonyl-CoA: 170.8
2-Methylbutyryl-CoA: 159.8
trans-1,2-CPDA: 137.5
Propionyl-CoA: 122.3
CHC-CoA: 104.3
Malonyl-CoA: 104.0
Isobutyryl-CoA: 85.7
Benzoyl-CoA: 72.3
Acetyl-CoA: 63.1
fatty_acid: 40.9
3-Methylbutyryl-CoA: 32.2
inactive: 30.8

PKS_AT (5214..5511): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 217.1
Ethylmalonyl-CoA: 208.4
Methoxymalonyl-CoA: 179.0
2-Methylbutyryl-CoA: 168.3
trans-1,2-CPDA: 126.8
Propionyl-CoA: 117.9
Malonyl-CoA: 116.4
CHC-CoA: 97.9
Isobutyryl-CoA: 91.7
Benzoyl-CoA: 67.6
Acetyl-CoA: 57.0
inactive: 41.5
3-Methylbutyryl-CoA: 39.4
fatty_acid: 21.5