BGC0000034 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000034 (Streptomyces sp. FR-008)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	138,203	levorin A3 / C06690 / FR-008-III / candicidin A / UNII-AP5PEF5W7U	Polyketide	100%

BGC0000034 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 138,203 nt. (total: 138,203 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 138,204
Label		BGC0000034

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000061.1	1.20	Polyketide	levorin A3, C06690, FR-008-III, candicidin A, UNII-AP5PEF5W7U	Streptomyces sp. FR-008
BGC0000034.1	1.20	Polyketide	candicidin	Streptomyces sp. FR-008
BGC0002031.1	0.68	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0001068.1	0.56	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001075.1	0.54	Terpene, Polyketide	aurachin RE	Rhodococcus erythropolis
BGC0001858.1	0.54	Polyketide	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000122.1	0.49	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0000056.1	0.48	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0000120.1	0.48	Polyketide	patulin	Penicillium expansum
BGC0001280.1	0.48	Polyketide	betaenone C, betaenone A	Phoma betae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000061.1	0.95	Polyketide	levorin A3, C06690, FR-008-III, candicidin A, UNII-AP5PEF5W7U	Streptomyces sp. FR-008
BGC0000034.1	0.95	Polyketide	candicidin	Streptomyces sp. FR-008
BGC0002031.1	0.67	Alkaloid	kitasetaline	Kitasatospora setae KM-6054
BGC0000067.1	0.66	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000120.1	0.64	Polyketide	patulin	Penicillium expansum
BGC0001276.1	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001340.1	0.64	Polyketide	byssochlamic acid	Byssochlamys fulva
BGC0001913.1	0.64	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0001164.1	0.64	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 926 - 138203:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal - pk - ohmal - ohmal - ohmal) + (redmal - redmal - ohmal - redmal) + (ccmal) + (Me-ohmal - Me-redmal - Me-ccmal) + (ccmal - ccmal - ccmal - ccmal - ccmal - ccmal) + (X - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

fscA

Search NORINE for peptide: strict or relaxed

AMP-binding (30..440): X

NRPSPredictor2: (unknown)

SVM prediction details:

NOTE: outside applicability domain

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: cFQvLaVngK
Nearest Stachelhaus code:: pip
Stachelhaus code match:: 50% (weak)

PKS_AT (1204..1498): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.7
inactive: 87.0
Methoxymalonyl-CoA: 62.5
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 46.8
Acetyl-CoA: 28.7
Ethylmalonyl-CoA: 27.8
Propionyl-CoA: 26.4
2-Methylbutyryl-CoA: 21.2
Benzoyl-CoA: 18.8
fatty_acid: 18.4
CHC-CoA: 9.7
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 6.6

fscC

PKS_AT (578..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.6
inactive: 106.2
Methoxymalonyl-CoA: 54.7
Methylmalonyl-CoA: 50.1
Propionyl-CoA: 29.8
Ethylmalonyl-CoA: 20.9
Benzoyl-CoA: 20.3
Isobutyryl-CoA: 20.0
Acetyl-CoA: 17.7
fatty_acid: 14.3
trans-1,2-CPDA: 12.3
2-Methylbutyryl-CoA: 11.4
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2320..2612): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.6
inactive: 112.3
Methoxymalonyl-CoA: 57.8
Methylmalonyl-CoA: 48.6
Propionyl-CoA: 28.5
Isobutyryl-CoA: 24.0
Benzoyl-CoA: 22.3
Acetyl-CoA: 21.5
Ethylmalonyl-CoA: 14.4
2-Methylbutyryl-CoA: 13.5
fatty_acid: 10.2
trans-1,2-CPDA: 6.0
CHC-CoA: 2.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (4061..4351): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.3
inactive: 99.0
Methoxymalonyl-CoA: 54.0
Methylmalonyl-CoA: 51.1
Acetyl-CoA: 26.2
Propionyl-CoA: 25.5
Benzoyl-CoA: 13.9
Isobutyryl-CoA: 12.4
Ethylmalonyl-CoA: 11.4
CHC-CoA: 11.3
2-Methylbutyryl-CoA: 10.4
fatty_acid: 8.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (5777..6069): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.9
inactive: 112.2
Methoxymalonyl-CoA: 56.4
Methylmalonyl-CoA: 53.7
Propionyl-CoA: 31.4
Acetyl-CoA: 24.0
Benzoyl-CoA: 19.8
trans-1,2-CPDA: 18.6
Isobutyryl-CoA: 18.2
Ethylmalonyl-CoA: 17.1
2-Methylbutyryl-CoA: 13.3
fatty_acid: 11.6
CHC-CoA: 3.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (7492..7783): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.4
inactive: 98.5
Methoxymalonyl-CoA: 52.6
Methylmalonyl-CoA: 44.6
Propionyl-CoA: 26.3
Acetyl-CoA: 20.9
Isobutyryl-CoA: 16.2
Benzoyl-CoA: 11.5
Ethylmalonyl-CoA: 9.6
trans-1,2-CPDA: 8.8
fatty_acid: 8.8
2-Methylbutyryl-CoA: 8.6
CHC-CoA: 2.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (9332..9621): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.5
inactive: 89.0
Methoxymalonyl-CoA: 40.7
Methylmalonyl-CoA: 40.0
Propionyl-CoA: 29.0
Acetyl-CoA: 25.1
Ethylmalonyl-CoA: 16.8
Isobutyryl-CoA: 9.8
Benzoyl-CoA: 9.8
2-Methylbutyryl-CoA: 6.6
CHC-CoA: 2.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

fscB

PKS_AT (573..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 212.0
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 155.9
trans-1,2-CPDA: 126.5
Propionyl-CoA: 120.3
Malonyl-CoA: 98.3
CHC-CoA: 95.6
Isobutyryl-CoA: 68.3
Benzoyl-CoA: 65.0
Acetyl-CoA: 51.2
fatty_acid: 44.1
inactive: 33.8
3-Methylbutyryl-CoA: 33.6

PKS_AT (2141..2438): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 212.0
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 155.9
trans-1,2-CPDA: 126.5
Propionyl-CoA: 120.3
Malonyl-CoA: 98.3
CHC-CoA: 95.6
Isobutyryl-CoA: 68.3
Benzoyl-CoA: 65.0
Acetyl-CoA: 51.2
fatty_acid: 44.1
inactive: 33.8
3-Methylbutyryl-CoA: 33.6

PKS_AT (4249..4546): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.1
Ethylmalonyl-CoA: 202.9
Methoxymalonyl-CoA: 169.3
2-Methylbutyryl-CoA: 131.7
trans-1,2-CPDA: 129.8
Propionyl-CoA: 108.2
Malonyl-CoA: 103.0
CHC-CoA: 98.2
Isobutyryl-CoA: 71.5
Benzoyl-CoA: 57.5
Acetyl-CoA: 41.0
inactive: 38.2
fatty_acid: 30.1
3-Methylbutyryl-CoA: 28.7

fscF

PKS_AT (547..843): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 226.9
inactive: 118.3
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 60.7
Isobutyryl-CoA: 26.9
Propionyl-CoA: 26.3
Benzoyl-CoA: 25.2
fatty_acid: 23.1
Ethylmalonyl-CoA: 20.6
CHC-CoA: 20.5
Acetyl-CoA: 20.2
trans-1,2-CPDA: 13.1
2-Methylbutyryl-CoA: 10.9
3-Methylbutyryl-CoA: 0.0

fscE

PKS_AT (550..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.9
inactive: 116.1
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 56.5
Isobutyryl-CoA: 27.3
Propionyl-CoA: 24.2
Benzoyl-CoA: 22.9
Acetyl-CoA: 21.2
Ethylmalonyl-CoA: 14.7
fatty_acid: 11.4
2-Methylbutyryl-CoA: 8.7
CHC-CoA: 8.4
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2592..2891): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.5
inactive: 93.3
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 46.0
Benzoyl-CoA: 20.3
Isobutyryl-CoA: 19.8
Ethylmalonyl-CoA: 17.0
Acetyl-CoA: 16.3
fatty_acid: 13.3
Propionyl-CoA: 11.2
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (4635..4931): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 231.4
inactive: 113.9
Methoxymalonyl-CoA: 69.2
Methylmalonyl-CoA: 61.2
Propionyl-CoA: 37.6
Isobutyryl-CoA: 28.3
fatty_acid: 22.5
Ethylmalonyl-CoA: 22.5
Benzoyl-CoA: 22.4
Acetyl-CoA: 21.2
2-Methylbutyryl-CoA: 16.1
CHC-CoA: 15.9
trans-1,2-CPDA: 12.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (6160..6458): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 221.5
inactive: 109.3
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 55.4
Propionyl-CoA: 25.5
CHC-CoA: 22.1
Acetyl-CoA: 21.0
2-Methylbutyryl-CoA: 20.3
Benzoyl-CoA: 20.2
Ethylmalonyl-CoA: 19.7
Isobutyryl-CoA: 16.8
fatty_acid: 16.0
trans-1,2-CPDA: 9.6
3-Methylbutyryl-CoA: 0.0

fscD

PKS_AT (562..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.6
inactive: 91.1
Methoxymalonyl-CoA: 52.8
Methylmalonyl-CoA: 42.8
Isobutyryl-CoA: 27.8
Propionyl-CoA: 26.7
Ethylmalonyl-CoA: 20.5
Benzoyl-CoA: 20.3
Acetyl-CoA: 17.4
2-Methylbutyryl-CoA: 16.2
CHC-CoA: 13.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2315..2622): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 215.9
inactive: 103.3
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 45.5
Propionyl-CoA: 34.7
Acetyl-CoA: 21.2
Isobutyryl-CoA: 20.3
Benzoyl-CoA: 16.9
2-Methylbutyryl-CoA: 16.4
fatty_acid: 7.4
CHC-CoA: 7.3
Ethylmalonyl-CoA: 6.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3874..4171): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 224.9
Methylmalonyl-CoA: 223.8
Methoxymalonyl-CoA: 181.4
2-Methylbutyryl-CoA: 163.1
trans-1,2-CPDA: 134.0
Propionyl-CoA: 118.2
CHC-CoA: 117.5
Malonyl-CoA: 109.9
Isobutyryl-CoA: 84.8
Benzoyl-CoA: 67.1
Acetyl-CoA: 52.7
3-Methylbutyryl-CoA: 37.3
inactive: 32.4
fatty_acid: 31.4

PKS_AT (5410..5717): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.1
inactive: 112.2
Methoxymalonyl-CoA: 61.9
Methylmalonyl-CoA: 54.9
Propionyl-CoA: 21.6
Isobutyryl-CoA: 16.8
Ethylmalonyl-CoA: 13.8
Acetyl-CoA: 13.6
Benzoyl-CoA: 10.6
fatty_acid: 9.6
CHC-CoA: 9.0
3-Methylbutyryl-CoA: 6.6
trans-1,2-CPDA: 3.5
2-Methylbutyryl-CoA: -0.2

PKS_AT (6937..7236): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.0
inactive: 117.7
Methoxymalonyl-CoA: 64.5
Methylmalonyl-CoA: 58.6
Propionyl-CoA: 27.4
Benzoyl-CoA: 22.0
Isobutyryl-CoA: 20.3
Acetyl-CoA: 19.7
Ethylmalonyl-CoA: 15.5
fatty_acid: 15.2
CHC-CoA: 12.8
2-Methylbutyryl-CoA: 12.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (8466..8767): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.8
inactive: 103.6
Methoxymalonyl-CoA: 65.5
Methylmalonyl-CoA: 59.3
Isobutyryl-CoA: 29.0
Ethylmalonyl-CoA: 26.8
Propionyl-CoA: 26.2
Benzoyl-CoA: 19.7
Acetyl-CoA: 18.3
fatty_acid: 17.7
CHC-CoA: 16.8
2-Methylbutyryl-CoA: 11.6
trans-1,2-CPDA: 11.4
3-Methylbutyryl-CoA: 0.0