BGC0000148 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000148 (Saccharopolyspora spinosa)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	80,161	High	A83543A/Spinosyn D	PKS:Type I

BGC0000148 - Region 1 - T1PKS

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Location: 1 - 80,161 nt. (total: 80,161 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 80,162
Label		BGC0000148

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

AAG23281.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

spnJ: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

spnN: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: aminotransferase glycosyltransferase methyltransferase

spnF: methyltransferase

smcogs: SMCOG1089: methyltransferase

spnG: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

spnL: methyltransferase

smcogs: SMCOG1089: methyltransferase

spnP: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

spnQ: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

spnR: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

spnS: methyltransferase

smcogs: SMCOG1248: methyltransferase

Ligases: synthase

spnA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

spnB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

spnC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

spnD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

spnE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000148.3	1.00	PKS	A83543A, Spinosyn D	Saccharopolyspora spinosa
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000148.3	1.00	PKS	A83543A, Spinosyn D	Saccharopolyspora spinosa
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 1110 - 80161:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - ohmal) + (redmal) + (Me-ohmal - ohmal) + (ccmal - ohmal - ohmal) + (Me-ccmal - ccmal - ccmal)
Non-colinear ordering:: spnA + spnB + spnC + spnD + spnE

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

spnA: mmal - mal

PKS_AT (528..824): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.7
Ethylmalonyl-CoA: 203.2
Methoxymalonyl-CoA: 178.8
trans-1,2-CPDA: 129.1
2-Methylbutyryl-CoA: 128.9
Malonyl-CoA: 123.9
CHC-CoA: 113.7
Propionyl-CoA: 104.4
Isobutyryl-CoA: 100.5
Benzoyl-CoA: 80.4
Acetyl-CoA: 70.6
fatty_acid: 30.5
inactive: 30.2
3-Methylbutyryl-CoA: 25.4

PKS_AT (1525..1826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.4
inactive: 86.7
Methoxymalonyl-CoA: 71.7
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 47.1
fatty_acid: 21.9
Acetyl-CoA: 20.4
Isobutyryl-CoA: 15.1
CHC-CoA: 14.7
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 10.8
2-Methylbutyryl-CoA: 9.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.3

spnB: mal

PKS_AT (572..873): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.4
inactive: 86.7
Methoxymalonyl-CoA: 71.7
Methylmalonyl-CoA: 57.2
Propionyl-CoA: 47.1
fatty_acid: 21.9
Acetyl-CoA: 20.4
Isobutyryl-CoA: 15.1
CHC-CoA: 14.7
Benzoyl-CoA: 13.0
trans-1,2-CPDA: 10.8
2-Methylbutyryl-CoA: 9.4
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.3

spnC: mmal - mal

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.7
Ethylmalonyl-CoA: 201.2
Methoxymalonyl-CoA: 174.0
2-Methylbutyryl-CoA: 128.5
trans-1,2-CPDA: 124.7
CHC-CoA: 100.2
Malonyl-CoA: 98.0
Propionyl-CoA: 92.7
Isobutyryl-CoA: 81.2
Benzoyl-CoA: 77.3
Acetyl-CoA: 43.9
3-Methylbutyryl-CoA: 40.2
inactive: 30.1
fatty_acid: 21.8

PKS_AT (2099..2398): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.0
inactive: 104.0
Methoxymalonyl-CoA: 73.8
Methylmalonyl-CoA: 56.4
Propionyl-CoA: 38.3
Isobutyryl-CoA: 33.1
fatty_acid: 21.7
trans-1,2-CPDA: 20.9
Acetyl-CoA: 19.4
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 12.7
CHC-CoA: 12.3
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.6

spnD: mal - mal - mal

PKS_AT (576..872): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 193.2
inactive: 87.8
Methoxymalonyl-CoA: 77.5
Methylmalonyl-CoA: 61.6
Propionyl-CoA: 39.2
Isobutyryl-CoA: 23.9
fatty_acid: 23.4
Acetyl-CoA: 23.2
trans-1,2-CPDA: 15.7
CHC-CoA: 15.0
2-Methylbutyryl-CoA: 12.4
Benzoyl-CoA: 11.9
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.9

PKS_AT (2320..2619): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 66.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.4
inactive: 86.4
Methoxymalonyl-CoA: 72.5
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 44.8
Isobutyryl-CoA: 31.6
Ethylmalonyl-CoA: 25.3
fatty_acid: 23.0
trans-1,2-CPDA: 20.8
Acetyl-CoA: 18.3
CHC-CoA: 18.2
2-Methylbutyryl-CoA: 12.8
Benzoyl-CoA: 12.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (3862..4160): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.5
Methoxymalonyl-CoA: 75.7
inactive: 65.4
Methylmalonyl-CoA: 55.7
Propionyl-CoA: 36.1
fatty_acid: 29.6
Isobutyryl-CoA: 29.6
Acetyl-CoA: 26.6
CHC-CoA: 21.9
2-Methylbutyryl-CoA: 18.3
Ethylmalonyl-CoA: 16.6
trans-1,2-CPDA: 15.0
Benzoyl-CoA: 14.1
3-Methylbutyryl-CoA: 8.3

spnE: mmal - mal - mal

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.9
Ethylmalonyl-CoA: 194.9
Methoxymalonyl-CoA: 163.1
2-Methylbutyryl-CoA: 130.5
trans-1,2-CPDA: 123.5
Malonyl-CoA: 106.8
CHC-CoA: 103.1
Isobutyryl-CoA: 100.5
Propionyl-CoA: 98.0
Benzoyl-CoA: 65.4
Acetyl-CoA: 42.7
3-Methylbutyryl-CoA: 41.7
fatty_acid: 31.2
inactive: 29.0

PKS_AT (2320..2617): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.6
inactive: 88.8
Methoxymalonyl-CoA: 69.7
Methylmalonyl-CoA: 54.6
Propionyl-CoA: 36.4
fatty_acid: 25.4
Acetyl-CoA: 24.7
Isobutyryl-CoA: 23.9
trans-1,2-CPDA: 16.6
CHC-CoA: 13.9
2-Methylbutyryl-CoA: 8.5
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -1.5
Benzoyl-CoA: -2.3

PKS_AT (4093..4390): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.7
inactive: 84.6
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 54.1
Propionyl-CoA: 36.7
trans-1,2-CPDA: 24.1
fatty_acid: 22.0
Acetyl-CoA: 18.9
Benzoyl-CoA: 16.9
Ethylmalonyl-CoA: 16.3
CHC-CoA: 11.5
2-Methylbutyryl-CoA: 10.8
Isobutyryl-CoA: 10.6
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor