BGC0000520

BGC0000520: lacticin 3147 A1 biosynthetic gene cluster from Lactococcus lactis

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Location: 27,744 - 37,224 nt. (total: 9,481 nt).
This entry is originally from NCBI GenBank AE001272.1.

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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General

Compounds

Genes

RiPP

History

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000520
Short description	lacticin 3147 A1 biosynthetic gene cluster from Lactococcus lactis
Status	Minimal annotation: yes A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	RiPP (Lanthipeptide)
Loci	NCBI GenBank: AE001272.1
Compounds	lacticin 3147 A1 lacticin 3147 A2
Species	Lactococcus lactis [taxonomy]
References	Lacticin 3147, a broad-spectrum bacteriocin which selectively dissipates the membrane potential. McAuliffe O et al., Appl Environ Microbiol (1998) PMID:9464377 Extensive post-translational modification, including serine to D-alanine conversion, in the two-component lantibiotic, lacticin 3147. Ryan MP et al., J Biol Chem (1999) PMID:10608807 Identification and overexpression of ltnl, a novel gene which confers immunity to the two-component lantibiotic lacticin 3147. McAuliffe O et al., Microbiology (Reading) (2000) PMID:10658659 Each peptide of the two-component lantibiotic lacticin 3147 requires a separate modification enzyme for activity. McAuliffe O et al., Microbiology (Reading) (2000) PMID:10974102 Regulation of immunity to the two-component lantibiotic, lacticin 3147, by the transcriptional repressor LtnR. McAuliffe O et al., Mol Microbiol (2001) PMID:11251818 Overproduction of wild-type and bioengineered derivatives of the lantibiotic lacticin 3147. Cotter PD et al., Appl Environ Microbiol (2006) PMID:16751576 Structural characterization of lacticin 3147, a two-peptide lantibiotic with synergistic activity. Martin NI et al., Biochemistry (2004) PMID:15023056 Mechanistic dissection of the enzyme complexes involved in biosynthesis of lacticin 3147 and nisin. Kuipers A et al., Appl Environ Microbiol (2008) PMID:18791001 Saturation mutagenesis of selected residues of the α-peptide of the lantibiotic lacticin 3147 yields a derivative with enhanced antimicrobial activity. Field D et al., Microb Biotechnol (2013) PMID:23433070 Lacticin 3147--biosynthesis, molecular analysis, immunity, bioengineering and applications. Suda S et al., Curr Protein Pept Sci (2012) PMID:21827422 Effect of broad- and narrow-spectrum antimicrobials on Clostridium difficile and microbial diversity in a model of the distal colon. Rea MC et al., Proc Natl Acad Sci U S A (2011) PMID:20616009

Chemical products information

lacticin 3147 A1 [synonyms: lacticin 3147]

(no structure information available)

lacticin 3147 A2 [synonyms: lacticin 3147]

(no structure information available)

List of genes involved in compound(s) production

Identifiers	Position	Product	Extra
AAC56053.1 ORF00035	27745 - 27924 (+)	L. lactis predicted coding region ORF00035	copy AA seq copy Nt seq
AAC56054.1 ORF00036	27963 - 28160 (+)	L. lactis predicted coding region ORF00036	copy AA seq copy Nt seq
AAC56011.1 ORF00037	28174 - 31116 (+)	lacticin 481/lactococcin biosynthesis protein LCNDR2	copy AA seq copy Nt seq
AAC56012.1 ORF00038	31143 - 33269 (+)	cytolysin B transport protein	copy AA seq copy Nt seq
AAC56013.1 ORF00039	33357 - 36140 (+)	lacticin 481/lactococcin biosynthesis protein LCNDR2	copy AA seq copy Nt seq
AAC56039.1 ORF00040	36169 - 37224 (+)	L. lactis predicted coding region ORF00040	copy AA seq copy Nt seq

RiPP-specific information

Subclass	Lanthipeptide
Cyclic?	no

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: 2QGQPUII4VGQV6OUMHRFLPYN, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4
3.0	Hidden contributor (ID: 3UOU7PODQJXIM6BEPUHHRRA5, no GDPR consent given).	Remove negative molecular mass Switched to AE001272.1 as an assembly of the plasmid containing the complete cluster
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

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