BGC0000991 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000991 (Sorangium cellulosum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	58,733	epothilone	NRP + Polyketide	33%

BGC0000991 - Region 1 - NRPS,T1PKS

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Location: 1 - 58,733 nt. (total: 58,733 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 58,734
Label		BGC0000991

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000991.1	1.12	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000989.1	0.87	NRP, Polyketide	epothilone	Sorangium cellulosum So0157-2
BGC0000990.1	0.81	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000988.1	0.69	NRP, Polyketide	epothilone B	Sorangium cellulosum
BGC0001164.1	0.56	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002057.1	0.56	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0000041.1	0.51	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000067.1	0.49	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001162.1	0.46	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001068.1	0.45	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000991.1	0.92	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000989.1	0.86	NRP, Polyketide	epothilone	Sorangium cellulosum So0157-2
BGC0000990.1	0.82	NRP, Polyketide	epothilone	Sorangium cellulosum
BGC0000988.1	0.77	NRP, Polyketide	epothilone B	Sorangium cellulosum
BGC0001164.1	0.68	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067.1	0.63	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001068.1	0.62	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002057.1	0.62	Polyketide	pateamine	Kiritimatiellaeota bacterium
BGC0000041.1	0.60	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001162.1	0.60	Polyketide	1-heptadecene	Moorea producens JHB

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 58733:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (cys) + (Me-ccmal) + (ohmal - ohmal - redmal - Me-redmal) + (Me-ohmal - Me-Me-ccmal) + (redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

epoA

PKS_AT (543..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.4
inactive: 132.5
Methoxymalonyl-CoA: 59.2
Propionyl-CoA: 47.2
Methylmalonyl-CoA: 44.3
Isobutyryl-CoA: 30.9
2-Methylbutyryl-CoA: 28.1
Acetyl-CoA: 25.3
Benzoyl-CoA: 24.7
Ethylmalonyl-CoA: 18.9
CHC-CoA: 13.6
fatty_acid: 11.2
3-Methylbutyryl-CoA: 9.1
trans-1,2-CPDA: 8.4

epoB

Search NORINE for peptide: strict or relaxed

AMP-binding (528..930): cys

NRPSPredictor2: cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: cys
Small clusters prediction:: cys
Single AA prediction:: cys

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code:: cys
Stachelhaus code match:: 100% (strong)

epoC

PKS_AT (539..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.1
Ethylmalonyl-CoA: 149.5
Methoxymalonyl-CoA: 137.8
Isobutyryl-CoA: 112.9
Benzoyl-CoA: 104.0
Malonyl-CoA: 93.5
trans-1,2-CPDA: 82.6
2-Methylbutyryl-CoA: 82.1
Propionyl-CoA: 77.1
CHC-CoA: 68.9
3-Methylbutyryl-CoA: 57.9
Acetyl-CoA: 55.5
inactive: 39.4
fatty_acid: 23.4

epoD

PKS_AT (563..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.2
inactive: 145.4
Methoxymalonyl-CoA: 74.0
Methylmalonyl-CoA: 59.1
Propionyl-CoA: 54.6
Isobutyryl-CoA: 45.2
2-Methylbutyryl-CoA: 34.1
Benzoyl-CoA: 32.6
Acetyl-CoA: 31.4
Ethylmalonyl-CoA: 29.3
CHC-CoA: 25.8
fatty_acid: 17.1
trans-1,2-CPDA: 9.7
3-Methylbutyryl-CoA: 2.7

PKS_AT (2056..2353): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.6
inactive: 137.6
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 59.7
Propionyl-CoA: 46.7
Isobutyryl-CoA: 38.7
Acetyl-CoA: 30.8
Benzoyl-CoA: 30.4
2-Methylbutyryl-CoA: 28.3
Ethylmalonyl-CoA: 28.2
CHC-CoA: 20.7
fatty_acid: 15.1
trans-1,2-CPDA: 4.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (3555..3852): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.2
inactive: 150.5
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 59.0
Propionyl-CoA: 56.1
Acetyl-CoA: 35.5
Isobutyryl-CoA: 35.4
Ethylmalonyl-CoA: 31.0
Benzoyl-CoA: 27.9
2-Methylbutyryl-CoA: 23.5
CHC-CoA: 20.2
fatty_acid: 18.2
trans-1,2-CPDA: 11.9
3-Methylbutyryl-CoA: 7.8

PKS_AT (5631..5928): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.9
Ethylmalonyl-CoA: 150.6
Methoxymalonyl-CoA: 141.5
Isobutyryl-CoA: 125.0
Benzoyl-CoA: 110.6
Malonyl-CoA: 101.7
2-Methylbutyryl-CoA: 93.4
trans-1,2-CPDA: 86.5
Propionyl-CoA: 83.6
Acetyl-CoA: 67.2
3-Methylbutyryl-CoA: 63.7
CHC-CoA: 62.2
inactive: 34.8
fatty_acid: 32.2

epoE

PKS_AT (561..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 179.3
Ethylmalonyl-CoA: 150.5
Methoxymalonyl-CoA: 137.4
Isobutyryl-CoA: 117.8
Malonyl-CoA: 100.6
Benzoyl-CoA: 99.3
2-Methylbutyryl-CoA: 91.2
Propionyl-CoA: 79.2
trans-1,2-CPDA: 69.4
CHC-CoA: 65.6
3-Methylbutyryl-CoA: 64.9
Acetyl-CoA: 60.7
inactive: 42.0
fatty_acid: 26.3

PKS_AT (2053..2350): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.8
Ethylmalonyl-CoA: 150.5
Methoxymalonyl-CoA: 135.4
Isobutyryl-CoA: 114.4
Benzoyl-CoA: 99.5
Malonyl-CoA: 98.8
2-Methylbutyryl-CoA: 95.5
Propionyl-CoA: 80.9
CHC-CoA: 68.6
trans-1,2-CPDA: 68.3
3-Methylbutyryl-CoA: 63.9
Acetyl-CoA: 62.0
inactive: 41.7
fatty_acid: 26.9

epoF

PKS_AT (556..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 199.7
inactive: 130.9
Methoxymalonyl-CoA: 63.1
Methylmalonyl-CoA: 48.9
Propionyl-CoA: 44.0
Isobutyryl-CoA: 37.0
2-Methylbutyryl-CoA: 30.1
Acetyl-CoA: 28.6
Benzoyl-CoA: 25.0
fatty_acid: 16.9
Ethylmalonyl-CoA: 16.2
CHC-CoA: 12.4
trans-1,2-CPDA: 5.3
3-Methylbutyryl-CoA: 0.0